AUTHOR=Boyne Alex , Yang Ming , Pulicani Sylvain , Feola Maria , Tkach Diane , Hong Robert , Duclert Aymeric , Duchateau Philippe , Juillerat Alexandre TITLE=Efficient multitool/multiplex gene engineering with TALE-BE JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.1033669 DOI=10.3389/fbioe.2022.1033669 ISSN=2296-4185 ABSTRACT=
TALE base editors are a recent addition to the genome editing toolbox. These molecular tools are fusions of a transcription activator-like effector domain (TALE), split-DddA deaminase halves, and an uracil glycosylase inhibitor (UGI) that have the distinct ability to directly edit double strand DNA, converting a cytosine (C) to a thymine (T). To dissect the editing rules of TALE-BE, we combined the screening of dozens of TALE-BE targeting nuclear genomic loci with a medium/high throughput strategy based on precise knock-in of TALE-BE target site collections into the cell genome. This latter approach allowed us to gain in depth insight of the editing rules