AUTHOR=Zhang Longbo , Getz Stephanie A. , Bordey Angelique 

TITLE=Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.814638

DOI=10.3389/fbioe.2021.814638

ISSN=2296-4185

ABSTRACT=<p>Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple neuronal populations within a circuit in a spatially and temporally regulated manner. In addition, this technique allows for morphophysiological investigations into neuronal development and connectivity following cell-specific gene manipulations. Here, we detail the dual <italic>in utero</italic> electroporation protocol.</p>