AUTHOR=Hwang Soonkyu , Lee Yongjae , Kim Ji Hun , Kim Gahyeon , Kim Hyeseong , Kim Woori , Cho Suhyung , Palsson Bernhard O. , Cho Byung-Kwan TITLE=Streptomyces as Microbial Chassis for Heterologous Protein Expression JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=Volume 9 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.804295 DOI=10.3389/fbioe.2021.804295 ISSN=2296-4185 ABSTRACT=

Heterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of expressing various secondary metabolite biosynthetic gene clusters and secretory enzymes. However, there are several issues that limit their use, including low yield, difficulty in genetic manipulation, and their complex cellular features. In this review, we summarize rational engineering approaches to optimizing the heterologous production of secondary metabolites and recombinant proteins in Streptomyces species in terms of genetic tool development and chassis construction. Further perspectives on the development of optimal Streptomyces chassis by the design-build-test-learn cycle in systems are suggested, which may increase the availability of secondary metabolites and recombinant proteins.