AUTHOR=Wang Zhaoyu , Li Yang , Li Mingyi , Zhang Xiaohui , Ji Qingxia , Zhao Xiaojuan , Bi Yanhong , Luo Si 

TITLE=Immobilized Fe3O4-Polydopamine-Thermomyces lanuginosus Lipase-Catalyzed Acylation of Flavonoid Glycosides and Their Analogs: An Improved Insight Into Enzymic Substrate Recognition

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.798594

DOI=10.3389/fbioe.2021.798594

ISSN=2296-4185

ABSTRACT=<p>The conversion of flavonoid glycosides and their analogs to their lipophilic ester derivatives was developed by nanobiocatalysts from immobilizing <italic>Thermomyces lanuginosus</italic> lipase (TLL) on polydopamine-functionalized magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (Fe<sub>3</sub>O<sub>4</sub>-PDA-TLL). The behavior investigation revealed that Fe<sub>3</sub>O<sub>4</sub>-PDA-TLL exhibits a preference for long chain length fatty acids (i.e., C10 to C14) with higher reaction rates of 12.6–13.9 mM/h. Regarding the substrate specificity, Fe<sub>3</sub>O<sub>4</sub>-PDA-TLL showed good substrate spectrum and favorably functionalized the primary OH groups, suggesting that the steric hindrances impeded the secondary or phenolic hydroxyl groups of substrates into the bonding site of the active region of TLL to afford the product.</p>