AUTHOR=Chen Hongrang , Zhang Haitao , Shen Yun , Dai Xingliang , Wang Xuanzhi , Deng Kunxue , Long Xiaoyan , Liu Libiao , Zhang Xinzhi , Li Yongsheng , Xu Tao 

TITLE=Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.684105

DOI=10.3389/fbioe.2021.684105

ISSN=2296-4185

ABSTRACT=<p><bold>Background:</bold> This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds.</p><p><bold>Methods:</bold> The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was used as the raw material to fabricate <italic>in-situ</italic> degradable nanofiber scaffolds. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and water vapor permeability test were used to evaluate the material properties of the scaffolds; cytotoxicity test was performed to evaluate material/residual solvent toxicity, and <italic>in situ</italic> tissue repair experiments in Balb/c mouse were performed.</p><p><bold>Results:</bold> The 3D printed handheld electrospinning device successfully fabricates PLA/gelatin nanofibrous membrane with uniformly layered nanofibers and good biocompatibility. Animal experiments showed that the mice in the experimental group had complete skin repair.</p><p><bold>Conclusions:</bold> The 3D printed handheld device can achieve <italic>in situ</italic> repair of full-thickness defects in mouse skin.</p>