AUTHOR=Cheng Jie , Tu Wenying , Luo Zhou , Gou Xinghua , Li Qiang , Wang Dan , Zhou Jingwen 

TITLE=A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.633028

DOI=10.3389/fbioe.2021.633028

ISSN=2296-4185

ABSTRACT=<p>Bioproduction of 5-aminovalerate (5AVA) from renewable feedstock can support a sustainable biorefinery process to produce bioplastics, such as nylon 5 and nylon 56. In order to achieve the biobased production of 5AVA, a 2-keto-6-aminocaproate-mediated synthetic pathway was established. Combination of L-Lysine α-oxidase from <italic>Scomber japonicus</italic>, α-ketoacid decarboxylase from <italic>Lactococcus lactis</italic> and aldehyde dehydrogenase from <italic>Escherichia coli</italic> could achieve the biosynthesis of 5AVA from biobased L-Lysine in <italic>E. coli</italic>. The H<sub>2</sub>O<sub>2</sub> produced by L-Lysine α-oxidase was decomposed by the expression of catalase KatE. Finally, 52.24 g/L of 5AVA were obtained through fed-batch biotransformation. Moreover, homology modeling, molecular docking and molecular dynamic simulation analyses were used to identify mutation sites and propose a possible trait-improvement strategy: the expanded catalytic channel of mutant and more hydrogen bonds formed might be beneficial for the substrates stretch. In summary, we have developed a promising artificial pathway for efficient 5AVA synthesis.</p>