AUTHOR=Ozer Eden , Alfonta Lital 

TITLE=Genetic Code Expansion of Vibrio natriegens

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2021.594429

DOI=10.3389/fbioe.2021.594429

ISSN=2296-4185

ABSTRACT=<p><italic>Escherichia coli</italic> has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium <italic>Vibrio natriegens</italic>. Different methodologies, well established in <italic>E. coli</italic>, are currently being adapted for <italic>V. natriegens</italic> in the hope to enable a much faster platform for general molecular biology studies. Amongst the vast technologies available for <italic>E. coli</italic>, genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for <italic>V. natriegens</italic> and demonstrate an unnatural amino acid incorporation into a protein for the first time in this organism.</p>