AUTHOR=Graf Michaela , Haas Thorsten , Teleki Attila , Feith André , Cerff Martin , Wiechert Wolfgang , Nöh Katharina , Busche Tobias , Kalinowski Jörn , Takors Ralf 

TITLE=Revisiting the Growth Modulon of Corynebacterium glutamicum Under Glucose Limited Chemostat Conditions

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.584614

DOI=10.3389/fbioe.2020.584614

ISSN=2296-4185

ABSTRACT=<p>Increasing the growth rate of the industrial host <italic>Corynebacterium glutamicum</italic> is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals. Here, we present comprehensive metabolic and transcriptional analysis of <italic>C. glutamicum</italic> ATCC 13032 growing under glucose limited chemostat conditions with μ = 0.2, 0.3, and 0.4 h<sup>–1</sup>. Intermediates of central metabolism mostly showed rising pool sizes with increasing growth. <sup>13</sup>C-metabolic flux analysis (<sup>13</sup>C-MFA) underlined the fundamental role of central metabolism for the supply of precursors, redox, and energy equivalents. Global, growth-associated, concerted transcriptional patterns were not detected giving rise to the conclusion that glycolysis, pentose-phosphate pathway, and citric acid cycle are predominately metabolically controlled under glucose-limiting chemostat conditions. However, evidence is found that transcriptional regulation takes control over glycolysis once glucose-rich growth conditions are installed.</p>