AUTHOR=Averianova Liudmila A. , Balabanova Larissa A. , Son Oksana M. , Podvolotskaya Anna B. , Tekutyeva Liudmila A. 

TITLE=Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.570828

DOI=10.3389/fbioe.2020.570828

ISSN=2296-4185

ABSTRACT=<p>Riboflavin is a crucial micronutrient that is a precursor to coenzymes flavin mononucleotide and flavin adenine dinucleotide, and it is required for biochemical reactions in all living cells. For decades, one of the most important applications of riboflavin has been its global use as an animal and human nutritional supplement. Being well-informed of the latest research on riboflavin production via the fermentation process is necessary for the development of new and improved microbial strains using biotechnology and metabolic engineering techniques to increase vitamin B2 yield. In this review, we describe well-known industrial microbial producers, namely, <italic>Ashbya gossypii</italic>, <italic>Bacillus subtilis</italic>, and <italic>Candida</italic> spp. and summarize their biosynthetic pathway optimizations through genetic and metabolic engineering, combined with random chemical mutagenesis and rational medium components to increase riboflavin production.</p>