AUTHOR=Wang Yangxin , Zhang Ningning , Tan Deming , Qi Zhenhui , Wu Changzhu 

TITLE=Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00714

DOI=10.3389/fbioe.2020.00714

ISSN=2296-4185

ABSTRACT=<p><italic>In situ</italic> immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. <italic>Candida antarctica</italic> lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO<sub>3</sub>)<sub>2</sub>⋅6H<sub>2</sub>O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.</p>