AUTHOR=Juillerat Alexandre , Tkach Diane , Yang Ming , Boyne Alex , Valton Julien , Poirot Laurent , Duchateau Philippe TITLE=Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00678 DOI=10.3389/fbioe.2020.00678 ISSN=2296-4185 ABSTRACT=

Here, we developed a straightforward methodology to generate TCRαβ negative (allogeneic) cells for CAR-T cell therapies. With an early and transient expression of an anti-CD3 CAR in the engineered donor T cells, we programmed these cells to self-eliminate the TCR+ cell population and obtained an ultrapure TCRαβ– population (99–99.9%) at the end of the CAR-T production. This novel and easy-to-implement procedure preserves the production yield and cell fitness and has the potential to streamline the manufacturing of “off-the-shelf” CAR T-cell therapies.