AUTHOR=Liu Zhen , Zhang Yinan , Sun Jianan , Huang Wen-Can , Xue Changhu , Mao Xiangzhao TITLE=A Novel Soluble Squalene-Hopene Cyclase and Its Application in Efficient Synthesis of Hopene JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00426 DOI=10.3389/fbioe.2020.00426 ISSN=2296-4185 ABSTRACT=
Hopene is an important precursor for synthesizing bioactive hopanoids with great commercial value. However, the chemical methods for synthesizing hopene are not efficient to date. Hopene is commonly obtained by extracting from plants or bacteria like other terpenoids, but the complicated extraction process is inefficient and unfriendly to the environment. Hopene can be biological synthesized by squalene-hopene cyclase (SHC) from squalene. However, hopene production by SHC remained at a low level until now. In this work, we found a novel SHC named OUC-SaSHC from