AUTHOR=Wang Qin , Zhong Chao , Xiao Han 

TITLE=Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00293

DOI=10.3389/fbioe.2020.00293

ISSN=2296-4185

ABSTRACT=<p>Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.</p>