AUTHOR=Cen Yu-Ke , Lin Jian-Guang , Wang You-Liang , Wang Jun-You , Liu Zhi-Qiang , Zheng Yu-Guo 

TITLE=The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00232

DOI=10.3389/fbioe.2020.00232

ISSN=2296-4185

ABSTRACT=<p>In recent years, there has been a noticeable increase in research interests on the <italic>Fusarium</italic> species, which includes prevalent plant pathogens and human pathogens, common microbial food contaminants and industrial microbes. Taken the advantage of gibberellin synthesis, <italic>Fusarium fujikuroi</italic> succeed in being a prevalent plant pathogen. At the meanwhile, <italic>F. fujikuroi</italic> was utilized for industrial production of gibberellins, a group of extensively applied phytohormone. <italic>F. fujikuroi</italic> has been known for its outstanding performance in gibberellin production for almost 100 years. Research activities relate to this species has lasted for a very long period. The slow development in biological investigation of <italic>F. fujikuroi</italic> is largely due to the lack of efficient research technologies and molecular tools. During the past decade, technologies to analyze the molecular basis of host-pathogen interactions and metabolic regulations have been developed rapidly, especially on the aspects of genetic manipulation. At the meanwhile, the industrial fermentation technologies kept sustained development. In this article, we reviewed the currently available research tools/methods for <italic>F. fujikuroi</italic> research, focusing on the topics about genetic engineering and gibberellin production.</p>