AUTHOR=Jiang Chengcheng , Liu Zhen , Sun Jianan , Mao Xiangzhao 

TITLE=Characterization of a Novel α-Neoagarobiose Hydrolase Capable of Preparation of Medium- and Long-Chain Agarooligosaccharides

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00470

DOI=10.3389/fbioe.2019.00470

ISSN=2296-4185

ABSTRACT=<p>α-Neoagarobiose hydrolase plays an important role in saccharification processes of marine biomass. In this study, an α-neoagarobiose hydrolase from <italic>Streptomyces coelicolor</italic> A3(2), designated as ScJC117, was identified, purified, and characterized. It has a sequence of 370 amino acids and belongs to the GH117 family. ScJC117 exhibited good activity under optimal hydrolysis conditions of pH 6.0 and 30°C, where it showed the <italic>K</italic><sub>m</sub> and <italic>k</italic><sub>cat</sub> for neoagarobiose of 11.57 mM and 0.48 s<sup>–1</sup>, respectively. ScJC117 showed the ability to hydrolyze neoagarooligosaccharides with the polymerization degrees of 2–14. A basis of catalytic activity toward the first α-1,3-glycosidic bond of the neoagarooligosaccharides from the non-reducing end, ScJC117 can be classified as an exo-type α-neoagarobiose hydrolase. These results suggested that ScJC117 could be used in the preparation of odd agarooligosaccharides (especially agaroheptaose-agaroundecaose) and 3,6-anhydro-L-galactose, which has a functional food additive potential. Moreover, ScJC117 can be used for comprehensive utilization of red algae.</p>