AUTHOR=Yan Pu , Zeng Yanjing , Shen Wentao , Tuo Decai , Li Xiaoying , Zhou Peng TITLE=Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00460 DOI=10.3389/fbioe.2019.00460 ISSN=2296-4185 ABSTRACT=
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as “Nimble Cloning” uses the restriction enzyme,