AUTHOR=CastaƱo-Cerezo Sara , FourniƩ Mathieu , Urban Philippe , Faulon Jean-Loup , Truan Gilles TITLE=Development of a Biosensor for Detection of Benzoic Acid Derivatives in Saccharomyces cerevisiae JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00372 DOI=10.3389/fbioe.2019.00372 ISSN=2296-4185 ABSTRACT=
4-hydroxybenzoic acid (pHBA) is an important industrial precursor of muconic acid and liquid crystal polymers whose production is based on the petrochemical industry. In order to decrease our dependency on fossil fuels and improve sustainability, microbial engineering is a particularly appealing approach for replacing traditional chemical techniques. The optimization of microbial strains, however, is still highly constrained by the screening stage. Biosensors have helped to alleviate this problem by decreasing the screening time as well as enabling higher throughput. In this paper, we constructed a synthetic biosensor, named sBAD, consisting of a fusion of the pHBA-binding domain of HbaR from