AUTHOR=Xu Lili , Fan Jianhua , Wang Quanxi 

TITLE=Omics Application of Bio-Hydrogen Production Through Green Alga Chlamydomonas reinhardtii

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00201

DOI=10.3389/fbioe.2019.00201

ISSN=2296-4185

ABSTRACT=<p>This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in <italic>Chlamydomonas reinhardtii</italic>. In this paper, critical genes (<italic>HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1</italic>) involved in H<sub>2</sub> metabolism were identified and analyzed for their function in H<sub>2</sub> accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H<sub>2</sub> production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H<sub>2</sub> metabolism. This review provides fundamental theory and an experimental basis for H<sub>2</sub> production, and further research effort is needed in this field.</p>