AUTHOR=Mohammed Danahe , Versaevel Marie , Bruyère Céline , Alaimo Laura , Luciano Marine , Vercruysse Eléonore , Procès Anthony , Gabriele Sylvain TITLE=Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=7 YEAR=2019 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00162 DOI=10.3389/fbioe.2019.00162 ISSN=2296-4185 ABSTRACT=

Cells and tissues can sense and react to the modifications of the physico-chemical properties of the extracellular environment (ECM) through integrin-based adhesion sites and adapt their physiological response in a process called mechanotransduction. Due to their critical localization at the cell-ECM interface, transmembrane integrins are mediators of bidirectional signaling, playing a key role in “outside-in” and “inside-out” signal transduction. After presenting the basic conceptual fundamentals related to cell mechanobiology, we review the current state-of-the-art technologies that facilitate the understanding of mechanotransduction signaling pathways. Finally, we highlight innovative technological developments that can help to advance our understanding of the mechanisms underlying nuclear mechanotransduction.