AUTHOR=Guo Wei , Huang Qiulan , Liu Hao , Hou Shaoli , Niu Suhao , Jiang Yi , Bao Xiaoming , Shen Yu , Fang Xu 

TITLE=Rational Engineering of Chorismate-Related Pathways in Saccharomyces cerevisiae for Improving Tyrosol Production

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2019.00152

DOI=10.3389/fbioe.2019.00152

ISSN=2296-4185

ABSTRACT=<p>Tyrosol is extensively used in the pharmaceutical industry as an important natural product from plants. In this study, an exogenous pathway involved in catalyzing tyrosine to tyrosol was introduced into <italic>Saccharomyces cerevisiae</italic>. Furthermore, The pyruvate decarboxylase gene <italic>pdc1</italic> was deleted to redirect the flux distribution at the pyruvate node, and a bifunctional NAD<sup>+</sup>-dependent fused chorismate mutase/prephenate dehydrogenase from <italic>E. coli</italic> (<italic>Ec</italic>TyrA) and its' tyrosine inhibition resistant mutant (<italic>Ec</italic>TyrA<sup>M53I/A354V</sup>) were heterologously expression in <italic>S. cerevisiae</italic> to tuning up the chorismate metabolism effectively directed the metabolic flux toward tyrosol production. Finally, the tyrosol yield of the engineered strain GFT-4 was improved to 126.74 ± 6.70 mg/g DCW at 48 h, increased 440 times compared with that of the control strain GFT-0 (0.28 ± 0.01 mg/g DCW). The new synergetic engineering strategy developed in this study can be further applied to increase the production of high value-added aromatic compounds derived from aromatic amino acid or shikimate in <italic>S. cerevisiae</italic>.</p>