AUTHOR=Messerschmidt Sonja J. , Schindler Daniel , Zumkeller Celine M. , Kemter Franziska S. , Schallopp Nadine , Waldminghaus Torsten 

TITLE=Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=4

YEAR=2016

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2016.00096

DOI=10.3389/fbioe.2016.00096

ISSN=2296-4185

ABSTRACT=<p>Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII in <italic>Escherichia coli</italic>. It is based on the replication mechanism of the secondary chromosome in <italic>Vibrio cholerae</italic>. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We probe the origin diversity of secondary chromosomes from <italic>Vibrionaceae</italic> by construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system.</p>