AUTHOR=Bozi Brenno , Rodrigues Jeane , Lima-Maximino Monica , de Siqueira-Silva Diógenes Henrique , Soares Marta Candeias , Maximino Caio 

TITLE=Social Stress Increases Anxiety-Like Behavior Equally in Male and Female Zebrafish

JOURNAL=Frontiers in Behavioral Neuroscience

VOLUME=15

YEAR=2021

URL=https://www.frontiersin.org/journals/behavioral-neuroscience/articles/10.3389/fnbeh.2021.785656

DOI=10.3389/fnbeh.2021.785656

ISSN=1662-5153

ABSTRACT=<p>Zebrafish anxiety-like behavior was assessed in the novel tank test after the formation of dominant-subordinate hierarchies. Ten pairs of animals were subjected to dyadic interactions for 5 days, and compared with control animals. After this period, a clear dominance hierarchy was established across all dyads, irrespective of sex. Social status affected parameters of anxiety-like behavior in the novel tank test, with subordinate males and females displaying more bottom-dwelling, absolute turn angle, and freezing than dominant animals and controls. The results suggest that subordinate male and female zebrafish show higher anxiety-like behavior, which together with previous literature suggests that subordination stress is conserved across vertebrates.</p>