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ORIGINAL RESEARCH article

Front. Aquac.
Sec. Production Biology
Volume 3 - 2024 | doi: 10.3389/faquc.2024.1450537

Exploring spore-forming lactic acid bacterium Heyndrickxia coagulans SANK70258 as a promising probiotic for red sea bream (Pagrus major)

Provisionally accepted
Shuji Shirakashi Shuji Shirakashi 1Aoki Ryuichiro Aoki Ryuichiro 2Yasuhisa Kobayashi Yasuhisa Kobayashi 1Masanori Aida Masanori Aida 3Ryouichi Yamada Ryouichi Yamada 3Akito Taniguchi Akito Taniguchi 1*
  • 1 Faculty of Agriculture, Kindai University, Nara, Nara, Japan
  • 2 Aquaculture Research Institute, Kindai University, Kagoshima, Japan
  • 3 Science & Innovation Center, Mitsubishi Chemical Corporation, Kanagawa, Japan

The final, formatted version of the article will be published soon.

    Enhancing fish growth and bolstering immune responses to diseases are of paramount concern in fish aquaculture. The use of probiotics has emerged as a promising strategy for addressing these challenges. The aim of this study was to investigate the characteristics (salinity tolerance, starchassimilating ability, organic acid production, β-galactosidase activity) of Heyndrickxia coagulans SANK70258 and its probiotic effects on red sea bream (Pagrus major). Our results indicate that H. coagulans SANK70258 exhibits a high salinity tolerance and thrives as a probiotic in marine environments. Moreover, its starch assimilation capability, organic acid production, and βgalactosidase activities suggest that it might play a role in enhancing the host's immune system. Over the 63-day experimental period, supplementation with H. coagulans SANK70258 significantly increased the survival rate of red sea bream compared with that of the experimental control group (vehicle starch for the probiotic) after the final experimental day, as determined by Kaplan-Meier analysis (log-rank test, p = 0.0228). Furthermore, supplementation affected the body length and weight of fish, but it did not influence digestive enzyme activities. The goblet cell abundance in the intestinal tract of fish supplemented with H. coagulans SANK70258 was significantly higher than that in the experimental control group (Welch's t-test, p = 0.03742). These results suggest that H. coagulans SANK70258 is a promising probiotic for enhancing marine fish aquaculture production.

    Keywords: Goblet cell, Heyndrickxia coagulans, Probiotics, Red sea bream, Spore-forming bacteria

    Received: 17 Jun 2024; Accepted: 29 Oct 2024.

    Copyright: © 2024 Shirakashi, Ryuichiro, Kobayashi, Aida, Yamada and Taniguchi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Akito Taniguchi, Faculty of Agriculture, Kindai University, Nara, 631-8505, Nara, Japan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.