AUTHOR=Tharmalingam Nagendran , Xu Shi , Felix Lewis Oscar , Roy Biswajit , Xian Ming , Mylonakis Eleftherios , Fuchs Beth Burgwyn TITLE=Gold complex compounds that inhibit drug-resistant Staphylococcus aureus by targeting thioredoxin reductase JOURNAL=Frontiers in Antibiotics VOLUME=2 YEAR=2023 URL=https://www.frontiersin.org/journals/antibiotics/articles/10.3389/frabi.2023.1179354 DOI=10.3389/frabi.2023.1179354 ISSN=2813-2467 ABSTRACT=Introduction

There is a significant need for new antimicrobial compounds that are effective against drug-resistant microbes. Thioredoxin reductase (TrxR) is critical in redox homeostasis and was identified as a potential drug target and confirmed through inhibition by compounds auranofin and Bay11-7085.

Methods

Additional TrxR inhibitors were designed and found to exhibit antimicrobial activity against Gram-positive (Enterococcus faecium and Staphylococcus aureus) and glutathione-deficient bacteria (Helicobacter pylori). Investigational compounds were tested for antimicrobial activity, anti-biofilm efficacy, target impact, and cytotoxicity.

Results

The first-generation molecules AU1 and AU5 inhibited TrxR activity and inhibited methicillin-resistant S. aureus strain MW2 with minimal inhibitory concentrations (MIC) of 0.125 and 0.5 μg/mL, respectively. In an S. aureus enzymatic assay, AU1 inhibited TrxR enzymatic activity in a dose-dependent manner causing a decrease in intracellular free thiols. In addition, biofilm studies demonstrated that AU1 and AU5 reduced biofilm formation at 1X MIC and disrupted mature biofilms at 4X MIC. Cytotoxicity profiles were created using human cell lines and primary cells with LD50 exceeding MICs by at least 12X.

Discussion

Thus, AU1 and AU5 were TrxR inhibitors that yielded low-concentration antimicrobial activity impacting S. aureus in planktonic and biofilm forms with limited toxic liability.