Dietary micronized hemp fiber enhances in vitro nutrient digestibility and cecal fermentation, antioxidant enzyme, lysosomal activity, and productivity in finisher broilers reared under thermal stress

Tossaporn Incharoen ^1* Manatsanun Nopparatmaitree ² Adisak Kongkeaw ¹ Keatisak Soisuwan ³ Wirot Likittrakulwong ⁴ Atichat Thongnum ⁵ Nima Norbu ⁶ Jigme Tenzin ⁷ Natphapat Supatsaraphokin ⁸ Juan J. Loor ⁹

• ¹ Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand
• ² Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Pechaburi, Thailand
• ³ Department of Animal Science, Faculty of Agriculture, Rajamagala University of Technology Srivijaya, Nakhon Si Thammarat, Thailand
• ⁴ Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand
• ⁵ Department of Animal Science and Fishery, Faculty of Sciences and Agricultural Technology, Rajamangala University of Technology Lanna (Phitsanulok Campus), Phitsanulok, Thailand
• ⁶ District Livestock Sector, District Administration Gasa, Khatoed, Gasa 14001, Bhutan., Gasa, Bhutan
• ⁷ Department of Animal Science, College of Natural Resources, Lobesa, Punakha 14001, Bhutan, Punakha, Bhutan
• ⁸ Firstly Tech Co. Ltd., Pathum Thani 12150, Thailand., Pathum Thani, Thailand
• ⁹ Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA., Urbana, United States

This study aimed to investigate the effects of dietary micronized hemp fiber (MHF) supplementation on in vitro nutrient digestibility, cecal fermentation, antioxidant enzyme activity, lysosomal function, and productivity in finisher broilers reared under a thermal environment. At 21 days of age, 210 broilers with uniform body weight were randomly allocated to three dietary treatments: a basal diet (CON), and diets supplemented with MHF at 0.75% (L-MHF) or 1.50% (H-MHF). Birds had ad libitum access to diets until 42 days of age. Compared with the CON, there was no effect (p > 0.05) of MHF supplementation on in vitro true digestibility of dry matter, organic matter, crude protein, ether extract, or gross energy. Similarly, gas production at various incubation times and the rate and extent of gas production from cecal fermentation did not differ (p > 0.05). However, cecal fermentation analysis revealed that total volatile fatty acid (VFA) concentrations, including acetic, propionic, and butyric acids, were significantly higher (p < 0.01) in the L-MHF and H-MHF groups. Microbiological analysis revealed increased (p < 0.01) total bacterial counts, lactic acid bacteria, and Enterococcus sp. populations, coupled with reduced E. coli counts in the L-MHF and H-MHF groups. There was a significant (p < 0.001) improvement in final body weight and average daily gain in the H-MHF group compared with the CON and L-MHF groups. Feed conversion ratio was lowest (p < 0.001) in the H-MHF group across all measured periods, while average daily feed intake remained unaffected (p > 0.05). Except for increased (p < 0.05) wing weight in the H-MHF group, dressing percentage or weight of major cuts did not differ (p > 0.05). Antioxidant enzyme activity was enhanced in the MHF-groups, with higher (p < 0.001) catalase, superoxide dismutase, and glutathione peroxidase activities observed in the H-MHF and L-MHF groups. However, lysozyme activity was reduced (p < 0.05) in the H-MHF group. In conclusion, dietary supplementation with MHF improved in vitro cecal fermentation profiles, antioxidant capacity, and productivity metrics in finisher broilers under thermal stress. These findings underscore the potential of MHF as a functional feed additive in broiler production.