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ORIGINAL RESEARCH article

Front. Anim. Sci.
Sec. Animal Nutrition
Volume 5 - 2024 | doi: 10.3389/fanim.2024.1465305

Effect of energy levels on liver oxidative state and gut microbiota of laying hens fed a low-protein diet

Provisionally accepted
Qianhui Mi Qianhui Mi 1Xu Deng Xu Deng 1*Peiying Wang Peiying Wang 2*Hongbin Pan Hongbin Pan 1Ying Huang Ying Huang 1Hong Hu Hong Hu 1Anjian Li Anjian Li 1Qiang Zhang Qiang Zhang 3*Shaoqing Wang Shaoqing Wang 1*
  • 1 Yunnan Agricultural University, Kunming, China
  • 2 Changchun Nanguan District Changtong Community Health Service Center, changchun, China
  • 3 WOD Poultry Research Institute, Beijing, beijing, China

The final, formatted version of the article will be published soon.

    The aim of this study was to investigate effect of energy levels on liver oxidative state and gut microbiota of laying hens fed a low-protein diet. A total of 216 laying hens (57-week-old with similar body weight) were randomly divided into 3 groups: low-energy and low-protein diet (LL: 10.73 MJ/kg), middle-energy and low-protein diet (ML: 11.15 MJ/kg), and high-energy and low-protein diet (HL: 11.57 MJ/kg) groups. The experiment lasted for 10 weeks. Results showed that the liver MDA level was higher, while the liver T-AOC level was lower in the HL group compared with LL and ML groups. The dominant phyla in LL group, ML group, and HL group were Bacteroidetes (38.08%, 39.69%, and 40.93%) and Firmicutes (16.78%, 18.37%, and 17.44%). At the genus level, Alistipes (9.45%) was abundant in the LL group. Bacteroides (14.46%), Phocaeicola (4.48%) and Precotella (2.95%) were highest in the ML group. Parabacteroides (1.78%) and Desulfovibrio (1.02%) were highest in the HL group. At the species level, Bacteroides fragilis (0.42%) is lowest in LL group, Desulfovibrio piger increased with the increase of energy. MDA was significantly and positively correlated with Methanobrevibacter woesei between ML group and HL groups (p < 0.05). T-AOC was highly significantly and positively correlated with Phocaeicola sp. Sa1YUN3 (p < 0.01). This study found that high-energy and low-protein diets might cause liver oxidative stress by gut microbes in the laying hens.

    Keywords: energy1, low protein diet2, laying hen3, liver4, Oxidative stress5, cecum microbiota6, metagenomics7

    Received: 16 Jul 2024; Accepted: 11 Oct 2024.

    Copyright: © 2024 Mi, Deng, Wang, Pan, Huang, Hu, Li, Zhang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xu Deng, Yunnan Agricultural University, Kunming, China
    Peiying Wang, Changchun Nanguan District Changtong Community Health Service Center, changchun, China
    Qiang Zhang, WOD Poultry Research Institute, Beijing, beijing, China
    Shaoqing Wang, Yunnan Agricultural University, Kunming, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.