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REVIEW article

Front. Anal. Sci.
Sec. Pharmaceutical analysis
Volume 4 - 2024 | doi: 10.3389/frans.2024.1490093

The Clinical Applications of Drugs and their Metabolites Analysis in Biological Fluids and Commonly Used Analytical Techniques for Bioanalysis: Review

Provisionally accepted
Biset Asrade Mekonnen Biset Asrade Mekonnen 1*Muluabay Getie Yizengaw Muluabay Getie Yizengaw 2Kebede Feyisa Aduna Kebede Feyisa Aduna 1
  • 1 Department of Pharmacy, School of Health Science, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia
  • 2 College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Amhara Region, Ethiopia

The final, formatted version of the article will be published soon.

    This study provides an overview of the clinical applications of drugs and their metabolites analysis in biological fluids and identifies commonly used analytical techniques for bioanalysis.Original open-access articles published between October 31, 2005, and October 31, 2020, in Google Scholar, MEDLINE, PubMed, and Embase were reviewed, and pertinent findings of the individual studies were pooled and presented using tables. This review was reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). Results: Fifteen studies met the eligibility criteria and were included in the review. These studies show that qualitative identification and quantitative determination of drugs and their metabolites in biological fluids are important for therapeutic drug monitoring (TDM), pharmacokinetic and pharmacodynamic studies, drug abuse control, and forensic and toxicological analyses. Spectroscopic, electrochemical, and hyphenated and nonhyphenated chromatographic techniques are used to analyse drugs and their metabolites in biological fluids. However, hyphenated techniques are the preferred analytical methods because of their sensitivity, selectivity, accuracy, reproducibility, efficiency, and rapid analysis.Bioanalysis is important for pharmacokinetic and pharmacodynamic studies, therapeutic drug monitoring, forensic and toxicological analyses, and drug abuse control using different bioanalytical techniques. However, hyphenated techniques are the most commonly used bioanalytical techniques. Bioanalysis of drugs and their metabolites needs to be improved to provide good medical and pharmaceutical care to patients, to confirm forensic and toxicological cases, and to control drug abuse.

    Keywords: drug, Medicine, bioanalysis, biological fluids, analytical techniques

    Received: 02 Sep 2024; Accepted: 11 Nov 2024.

    Copyright: © 2024 Mekonnen, Yizengaw and Aduna. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Biset Asrade Mekonnen, Department of Pharmacy, School of Health Science, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.