AUTHOR=Calabrese Valentina , Salvador Arnaud , Clément Yohann , Brunet Thomas Alexandre , Espeyte Anabelle , Chaumot Arnaud , Geffard Olivier , Degli-Esposti Davide , Ayciriex Sophie TITLE=Challenges and perspectives in MS-based omics approaches for ecotoxicology studies: An insight on Gammarids sentinel amphipods JOURNAL=Frontiers in Analytical Science VOLUME=3 YEAR=2023 URL=https://www.frontiersin.org/journals/analytical-science/articles/10.3389/frans.2023.1118494 DOI=10.3389/frans.2023.1118494 ISSN=2673-9283 ABSTRACT=

The aquatic environment is one of the most complex biosystems, as organism at all trophic levels may be exposed to a multitude of pollutants. As major goals, ecotoxicology typically investigates the impact of toxic pollutants on the ecosystems through the study of sentinel organisms. Over the past decades, Mass Spectrometry (MS)-based omics approaches have been extended to sentinel species both in laboratory and field exposure conditions. Single-omics approaches enable the discovery of biomarkers mirroring the health status of an organism. By covering a restricted set of the molecular cascade, they turn out to only partially satisfy the understanding of complex ecotoxicological effects. In contrast, a more complete understanding of the ecotoxicity pathways can be accessed through multi-omics approaches. In this perspective, we provide a state-of-the-art and a critical evaluation on further developments in MS-based single and multi-omics studies in aquatic ecotoxicology. As case example, literature regarding Gammarids freshwater amphipods, non-model sentinel organisms sensitive to pollutants and environmental changes and crucial species for downstream ecosystems, will be reviewed.