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ORIGINAL RESEARCH article
Front. Amphib. Reptile Sci.
Sec. Conservation
Volume 3 - 2025 | doi: 10.3389/famrs.2025.1547830
This article is part of the Research Topic Biological Invasions: Reptiles and Amphibians View all 3 articles
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Efficient and effective monitoring is essential for informing conservation efforts and determining where to focus management actions. Newly arriving invasive and declining native taxa are often the focus of conservation programs, but these species are inherently difficult to monitor and detect. A key challenge to effective monitoring is when a species is present at a location but goes undetected, creating uncertainty about whether the species is truly absent or present but unobserved. Estimating detection improves the efficacy of monitoring methods and accurately informs conservation efforts. In this study, we applied occupancy modeling to estimate method-specific detection probabilities for two focal amphibian species: the native northern leopard frog (Lithobates pipiens) and invasive American bullfrog (Lithobates catesbeianus). We evaluated three monitoring techniques: automated acoustic recorders, eDNA, and visual encounter surveys at 38 breeding sites in Colorado's South Platte River Basin, USA, an area where northern leopard frogs are declining, and bullfrogs are expanding. We investigated how local environmental conditions (temperature and pH) and survey-specific choices (survey date, number of observers, search time, and liters of filtered water) influenced detection probabilities for each species and method. Our results showed that northern leopard frog detection probability increased slightly with longer visual search times and eDNA detection probability was highest in neutral pH conditions. Acoustic detection of northern leopard frog breeding calls peaked in early spring. For bullfrogs, eDNA detection improved with liters of filtered water and both eDNA and visual detection increased with water temperature. Bullfrog breeding call detection was highest in mid to late summer. Northern leopard frog and bullfrog distinct life history traits related to breeding habitat preferences, behaviors, and seasonal emergence explained differences in their detection probabilities.
Keywords: invasive species, conservation, Monitoring methods, Detection probability, eDNA, automated acoustic recorders, Lithobates catesbeianus (American bullfrog), Lithobates pipiens
Received: 18 Dec 2024; Accepted: 03 Apr 2025.
Copyright: © 2025 Davis, Bailey and Wright. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Waverly Davis, Colorado State University, Fort Collins, United States
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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