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ORIGINAL RESEARCH article
Front. Agron.
Sec. Disease Management
Volume 7 - 2025 | doi: 10.3389/fagro.2025.1525588
This article is part of the Research TopicDisease Resistance In Hordeum SpeciesView all articles
Genome Wide Association Mapping of Net Form Net Blotch resistance in Barley at Seedling and Adult Plant Stages
Provisionally accepted- 1International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
- 2Field Crop Development Centre (FCDC), Lacombe, Alberta, Canada
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The net form net blotch (NFNB) caused by Pyrenophora teres f. sp. teres (Ptt) is an emerging barley disease in several countries. It causes severe yield and quality losses due to infection of leaves, kernels, and stems. Owing to inherent genetic diversity of Ptt, the incorporation of qualitative and quantitative resistance is important to obtain barley cultivars with durable resistance to NFNB. For this purpose, an association mapping panel named as HI-AM (High Input Association Mapping Panel) was screened for resistance to NFNB at the seedling stage with two virulent Moroccan Ptt isolates (Ptt40-3, Ptt45-3) under controlled conditions, and at the adult plant stage at four hot spot locations in Morocco during different cropping seasons (2016-17 and 2017-18). Genome wide association mapping (GWAM) was conducted using 13,182 PAV (presence or absence variations) and 6,311 SNP markers (DaRTSeq) for mapping of seedling and adult plant resistance quantitative trait loci (QTL). GWAM analysis revealed 19 QTL for seedling stage and 35 QTL for adult plant stage resistance. Of the 54 QTL detected, 38 QTL from this study overlapped with previously reported QTL while 16 QTL were novel. Furthermore, 2 common SRT and 6 common adult plant stage QTL were detected while only 3 QTL overlapped for both growth stages.Seedling stage QTL explained together 40% of the genetic variance for seedling resistance to isolate Ptt40-3, and 69% for isolate Ptt45-3, whereas the genetic variance of QTL for adult plant stage resistance ranged from 35% to 85%. This panel was previously used for other GWAM studies including resistance to spot blotch and stripe rust of barley. By mapping of significant markers for three different diseases on the Morex genome version 3.0, we have identified 13 common QTL associated with resistance to net blotch and spot blotch, and 3 QTL associated with resistance to all three diseases. The identification and introgression of common QTL conditioning resistance to three pathogens could help in attaining durable disease resistance in barley in North Africa.
Keywords: barley, Net form net blotch, Resistance, GWAM, QTL
Received: 10 Nov 2024; Accepted: 14 Apr 2025.
Copyright: © 2025 Rehman, Al-Jaboobi, Verma, Sanchez-Garcia and Visioni. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Sajid Rehman, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
Andrea Visioni, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
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