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ORIGINAL RESEARCH article

Front. Agron.
Sec. Weed Management
Volume 6 - 2024 | doi: 10.3389/fagro.2024.1456168
This article is part of the Research Topic Innovative Technology and Techniques for Effective Weed Control View all 4 articles

Oxidative damage in Echinochloa crus-galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination

Provisionally accepted
Naphat Somala Naphat Somala 1*Nutcha Manichart Nutcha Manichart 1*CHAMROON LAOSINWATTANA CHAMROON LAOSINWATTANA 1*Pattharin Wichittrakarn Pattharin Wichittrakarn 2*Kaori Yoneyama Kaori Yoneyama 3*Montinee Teerarak Montinee Teerarak 1*Nawasit Chotsaeng Nawasit Chotsaeng 4*
  • 1 School of Agricultural Technology King Mongut's Institute of Technology Ladkrabang, Bangkok, Thailand, Thailand
  • 2 King Mongkut's Institute of Technology Ladkrabang, Lat Krabang District, Thailand
  • 3 Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama City, Japan
  • 4 Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Lat Krabang District, Thailand

The final, formatted version of the article will be published soon.

    This study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl acetate (EtOAc) fraction most greatly reduced seed germination (81.01%), root length (89.18%), and shoot length (84.74%) compared to the control. Chemical characterization using gas chromatographymass spectrometry revealed major constituents of linoleic acid, butyl ester (9.69%), hexadecanoic acid (7.99%), and 14-pentadecenoic acid (7.86%). With regard to physiological and biochemical indexes, treated seeds exhibited lower imbibition, significantly decreased α-amylase (EC 3.2.1.1) activity (p<0.05), and increased accumulation of malondialdehyde (85.52%) and hydrogen peroxide (141.10%). Moreover, activity of the antioxidant enzymes superoxide dismutase (EC 1.15.1.1) and guaiacol peroxidase (EC 1.11.1.7) was upregulated (67.24 and 61.62%, respectively), while catalase (EC 1.11.1.6) activity was downregulated (-33.75%). The inference is that an imbalance in ROS levels combined with reduced antioxidant potential drives the gradual accumulation of oxidative damage in seed cells and consequent loss of seed viability. All told, these results confirm the Diaporthe extract to induce oxidative stress and inhibit antioxidant enzymes. This study clearly demonstrates the oxidative damage associated with Diaporthe allelochemicals.

    Keywords: Allelochemicals, Barnyardgrass, Diaporthe sp., Oxidative damage, phytotoxicity

    Received: 28 Jun 2024; Accepted: 06 Nov 2024.

    Copyright: © 2024 Somala, Manichart, LAOSINWATTANA, Wichittrakarn, Yoneyama, Teerarak and Chotsaeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Naphat Somala, School of Agricultural Technology King Mongut's Institute of Technology Ladkrabang, Bangkok, Thailand, Thailand
    Nutcha Manichart, School of Agricultural Technology King Mongut's Institute of Technology Ladkrabang, Bangkok, Thailand, Thailand
    CHAMROON LAOSINWATTANA, School of Agricultural Technology King Mongut's Institute of Technology Ladkrabang, Bangkok, Thailand, Thailand
    Pattharin Wichittrakarn, King Mongkut's Institute of Technology Ladkrabang, Lat Krabang District, 10520, Thailand
    Kaori Yoneyama, Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama City, 338-8570, Japan
    Montinee Teerarak, School of Agricultural Technology King Mongut's Institute of Technology Ladkrabang, Bangkok, Thailand, Thailand
    Nawasit Chotsaeng, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Lat Krabang District, 10520, Thailand

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