AUTHOR=Berais-Rubio Andrés , Morel Revetria María A. , Giménez Matías , Signorelli Santiago , Monza Jorge 

TITLE=Competitiveness and symbiotic efficiency in alfalfa of Rhizobium favelukesii ORY1 strain in which homologous genes of peptidases HrrP and SapA that negatively affect symbiosis were identified

JOURNAL=Frontiers in Agronomy

VOLUME=4

YEAR=2023

URL=https://www.frontiersin.org/journals/agronomy/articles/10.3389/fagro.2022.1092169

DOI=10.3389/fagro.2022.1092169

ISSN=2673-3218

ABSTRACT=<p>In acid soils, <italic>Rhizobium favelukesii</italic> strains, known as Oregon-like strains, are a potential risk for alfalfa production given their parasitic behaviour. In this study, we isolated five parasitic strains (ORY1 to ORY5) from alfalfa nodules grown in Uruguayan acid soils, with a 99.7% and a 100% 16S rRNA gene sequence identity to <italic>R. favelukesii</italic> type strain of LUP83. The BOX profiles of the five isolates showed two different patterns, suggesting some diversity among these acid-tolerant isolates. The genome sequence analysis of <italic>R. favelukesii</italic> strains ORY1, LPU83, and Or191 showed that they have around 87.5% of common coding genes, including the symbiotic genes. Moreover, the phylogenetic analysis of ORY1 symbiotic genes <italic>nifH</italic>, <italic>nifD</italic>, <italic>nifK</italic>, <italic>nodA</italic>, <italic>nodB</italic>, and <italic>nodD</italic> were related to the symbiotic genes of <italic>E. meliloti</italic>. We teste ORY1 competitiveness by inoculating seeds with 99:1 and 1:99 ratios of ORY1::<italic>gusA</italic>/<italic>E. meliloti</italic> U143. In both treatments, ORY1::<italic>gusA</italic> occupied more than 50% of nodules, evidencing its high competitiveness. However, the aerial biomass in these treatments was remarkably different, suggesting that the nodules induced by the efficient strain are essential to provide enough N for optimal plant growth. These findings support the needing of inoculating in areas where inefficient strains are likely to be present. Finally, we found three genes that encode amino acid sequences for domains of M16 peptidases (with homology to bacterial <italic>hrrP</italic> and <italic>sapA</italic> genes), two of them were contiguous and located in an accessory plasmid, whereas the other one was a chromosomal gene. These genes are likely to be involved in the parasitic behaviour of ORY1 strain.</p>