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ORIGINAL RESEARCH article

Front. Aging Neurosci.
Sec. Alzheimer's Disease and Related Dementias
Volume 17 - 2025 | doi: 10.3389/fnagi.2025.1478199

Lingguizhugan Decoction enhances autophagy of Alzheimer's disease via regulating the mTOR/ p70s6K pathway in vivo and in vitro

Provisionally accepted
Chunxiang Zhou Chunxiang Zhou *Xiaojing Chen Xiaojing Chen Qingrong Tian Qingrong Tian Min Gao Min Gao Xibin Zhou Xibin Zhou
  • Nanjing University of Chinese Medicine, Nanjing, China

The final, formatted version of the article will be published soon.

    Lingguizhugan decoction (LGZG) has been reported to treat Alzheimer's disease (AD) by antiinflammatory and transporting amyloid-β (Aβ). Using APP/PS1 transgenic mice as in vivo model and gave LGZG decoction by oral gavage. Using Aβ25-35-induced SH-SY5Y cells as in vitro model and then added LGZG medicated serum (LMS) to observe the regulatory effect of LGZG on AD autophagy-related pathways. Morris water maze (MWM) was used to evaluate the mice's learning and memory ability. Mice's hippocampus tissue sections were stained immunohistochemically to observe hippocampal Aβ deposition. Transmission electron microscopy monitored autophagosomes and autolysosomes. Western blot analysis measured protein expression levels of beclin-1, p62 and light chain 3Ⅱ (LC3 Ⅱ) and mTOR signaling. Results: LGZG could greatly improve learning and memory ability of APP/PS1 mice, and enhance autophagy in vitro and in vivo.LGZG increased the levels of beclin-1 and LC3 Ⅱ and decreased the levels of p62. In conclusion, LGZG enhanced autophagy and showed therapeutic potential in AD by inhibiting mTOR/p70s6K signaling.

    Keywords: Autophagy, Lingguizhugan decoction, mTOR, Chinese medicine pharmacology, Neurodegenerative Diseases, Complementary medicine

    Received: 09 Aug 2024; Accepted: 08 Jan 2025.

    Copyright: © 2025 Zhou, Chen, Tian, Gao and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chunxiang Zhou, Nanjing University of Chinese Medicine, Nanjing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.