AUTHOR=Sugiura Ayumu , Shimizu Tatsuhiro , Kameyama Takeshi , Maruo Tomohiko , Kedashiro Shin , Miyata Muneaki , Mizutani Kiyohito , Takai Yoshimi TITLE=Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging JOURNAL=Frontiers in Aging Neuroscience VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2020.609911 DOI=10.3389/fnagi.2020.609911 ISSN=1663-4365 ABSTRACT=

The hypothalamus plays a central role in homeostasis and aging. The hypothalamic arcuate nucleus (ARC) controls homeostasis of food intake and energy expenditure and retains adult neural stem cells (NSCs)/progenitor cells. Aging induces the loss of NSCs and the enhancement of inflammation, including the activation of glial cells in the ARC, but aging-associated alterations of the hypothalamic cells remain obscure. Here, we identified Sox2 and NeuN double-positive cells in a subpopulation of cells in the mouse ARC. These cells were reduced in number with aging, although NeuN-positive neuronal cells were unaltered in the total number. Diet-induced obesity mice fed with high-fat diet presented a similar hypothalamic alteration to aged mice. This study provides a new insight into aging-induced changes in the hypothalamus.