AUTHOR=Liang Zhigang , Liu Zhuli , Sun Xuwen , Tao Manli , Xiao Xiao , Yu Guoping , Wang Xiaomin 

TITLE=The Effect of Fucoidan on Cellular Oxidative Stress and the CatD-Bax Signaling Axis in MN9D Cells Damaged by 1-Methyl-4-Phenypyridinium

JOURNAL=Frontiers in Aging Neuroscience

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2018.00429

DOI=10.3389/fnagi.2018.00429

ISSN=1663-4365

ABSTRACT=<p><bold>Background:</bold> The purpose of this study was to investigate the impact of fucoidan (FUC) on the oxidative stress response and lysosomal apoptotic pathways in the Parkinson disease (PD) cell model.</p><p><bold>Methods:</bold> The Dopaminergic nerve precursor cell line(MN9D) cells that served as a PD model in this study underwent damage induced by 100 μM 1-methyl-4-phenyl pyridine (MPP<sup>+</sup>). Cell viability was assessed after FUC treatment and intracellular SOD GSH was measured via immunofluorescence assay. Cellular changes in cathepsin D, Autophagy marker Light Chain 3-II (LC3-II), and apoptotic protein Bax were assessed by Western blot. The expression of Cat D, LC3-II, and B cell lymphoma-2-associated x protein (Bax) was also measured after addition of the cathepsin inhibitor, pepstatin A.</p><p><bold>Results:</bold> The results indicated that MN9D cell viability decreased by 50% within 24 h after 100 μM MPP<sup>+</sup> induced toxicity. Pretreatment with 100 μM Fucoidan reduced cellular expression of LC3-II and CatD in 3 h and suppressed the induction of Bax protein. After pepstatin A treatment, Bax expression was significantly downregulated.FUC reversed the reduction of superoxide dismutase (SOD) L-Glutathione(GSH), decreased cell viability, and apoptosis induced by MPP<sup>+</sup> in 6 h, suggesting that Fucoidan can attenuate damage to MN9D cells induced by MPP<sup>+</sup>.</p><p><bold>Conclusions:</bold> Fucoidan protected lysosomes, reduced the expression of LC3-II, inhibited the expression of CatD-Bax and the oxidative stress response, suppressed apoptosis, and thus conferred protective effects for dopaminergic neural cells. FUC may have neuroprotective effects on PD and further research is needed.</p>