AUTHOR=Fernández-Eulate Gorka , Alberro Ainhoa , Muñoz-Culla Maider , Zulaica Miren , Zufiría Mónica , Barandiarán Myriam , Etxeberria Igone , Yanguas José Javier , Gallardo Maria Mercedes , Soberón Nora , Lacosta Ana María , Pérez-Grijalba Virginia , Canudas Jesús , Fandos Noelia , Pesini Pedro , Sarasa Manuel , Indakoetxea Begoña , Moreno Fermin , Vergara Itziar , Otaegui David , Blasco Maria , López de Munain Adolfo 

TITLE=Blood Markers in Healthy-Aged Nonagenarians: A Combination of High Telomere Length and Low Amyloidβ Are Strongly Associated With Healthy Aging in the Oldest Old

JOURNAL=Frontiers in Aging Neuroscience

VOLUME=10

YEAR=2018

URL=https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2018.00380

DOI=10.3389/fnagi.2018.00380

ISSN=1663-4365

ABSTRACT=<p>Many factors may converge in healthy aging in the oldest old, but their association and predictive power on healthy or functionally impaired aging has yet to be demonstrated. By detecting healthy aging and in turn, poor aging, we could take action to prevent chronic diseases associated with age. We conducted a pilot study comparing results of a set of markers (peripheral blood mononuclear cell or PBMC telomere length, circulating Aβ peptides, anti-Aβ antibodies, and ApoE status) previously associated with poor aging or cognitive deterioration, and their combinations, in a cohort of “neurologically healthy” (both motor and cognitive) nonagenarians (<italic>n</italic> = 20) and functionally impaired, institutionalized nonagenarians (<italic>n</italic> = 38) recruited between 2014 and 2015. We recruited 58 nonagenarians (41 women, 70.7%; mean age: 92.37 years in the neurologically healthy group vs. 94.13 years in the functionally impaired group). Healthy nonagenarians had significantly higher mean PBMC telomere lengths (mean = 7, <italic>p</italic> = 0.001), this being inversely correlated with functional impairment, and lower circulating Aβ40 (total in plasma fraction or TP and free in plasma fraction or FP), Aβ42 (TP and FP) and Aβ17 (FP) levels (FP40 131.35, <italic>p</italic> = 0.004; TP40 299.10, <italic>p</italic> = 0.007; FP42 6.29, <italic>p</italic> = 0.009; TP42 22.53, <italic>p</italic> = 0.019; FP17 1.32 <italic>p</italic> = 0.001; TP17 4.47, <italic>p</italic> = 0.3), after adjusting by age. Although healthy nonagenarians had higher anti-Aβ40 antibody levels (net adsorbed signal or NAS ± SD: 0.211 ± 0.107), the number of participants that pass the threshold (NAS &gt; 3) to be considered as positive did not show such a strong association. There was no association with ApoE status. Additionally, we propose a “<italic>Composite Neurologically Healthy Aging Score”</italic> combining TP40 and mean PBMC telomere length, the strongest correlation of measured biomarkers with neurologically healthy status in nonagenarians (AUC = 0.904).</p>