AUTHOR=Vasan Aditya , Magaram Uri , Patel Janki , Friend James , Chalasani Sreekanth H. 

TITLE=Integrating ultrasonic neuromodulation with fiber photometry

JOURNAL=Frontiers in Acoustics

VOLUME=1

YEAR=2023

URL=https://www.frontiersin.org/journals/acoustics/articles/10.3389/facou.2023.1326759

DOI=10.3389/facou.2023.1326759

ISSN=2813-8082

ABSTRACT=<p>Ultrasound has been used to modulate neural activity in rodents and primates; however, combining ultrasound stimulation with <italic>in vivo</italic> imaging in freely moving animals has been challenging. Here, we design and validate a transducer to overcome these challenges in the rodent. We develop a head-mounted ultrasound transducer that can be combined with a fiber photometry system. This combination allows us to monitor ultrasound-evoked responses in striatal neurons in awake and freely moving animals. Together, this system allows for a high-resolution analysis of ultrasound-evoked biology at the level of both neural circuits and behavior in freely moving animals, critical to providing a mechanistic understanding of ultrasound neuromodulation.</p>