- 1Plant Biology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United States
- 2Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, United States
- 3Department of Biology, The Pennsylvania State University, University Park, PA, United States
- 4Institute for Sustainable Plant Protection, National Research Council (CNR), Turin, Italy
Plants have a highly sophisticated endomembrane system targeted by plant viruses for cell-to-cell movement. The movement protein (MP) of ourmia melon virus (OuMV) is delivered to plasmodesmata (PD) and forms tubules to facilitate cell-to-cell movement. In our previous work, we identified the sites of OuMV for correct subcellular localization; however, the pathways involved in PD-targeting of OuMV MP are largely unclear, and their identification was the aim of this work. In this study, we demonstrate that OuMV MP localizes to the trans-Golgi network (TGN) but not to the multivesicular body/prevacuolar compartment or Golgi, and carries two putative sorting motifs, a tyrosine (Y) and a dileucine (LL) motif, near its N-terminus. Substitutions in these motifs result in loss of OuMV infectivity in Nicotiana benthamiana and Arabidopsis. Live cell imaging of GFP-labeled sorting motif mutants reveals that both motifs are required for targeting OuMV MP to PD and for efficient systemic infection but show differences in functionality. Moreover, co-immunoprecipitation assays coupled with mass spectrometry identified a series of host factors that could interact with the OuMV MP and link the MP with various pathways, in particular vesicle trafficking and membrane lipids. Our findings delineate the trafficking route of OuMV MP for PD-targeting and elucidate host factors potentially recruited by OuMV MP that could be modified to impair OuMV infection.
Introduction
Viral movement proteins (MPs) are critical for mediating virus movement through plasmodesmata (PD) as the size of virus nucleic acids and virions does not allow for passive intercellular movement. Indeed, localization of viral MPs to PD has been demonstrated, and some MPs can increase the PD size exclusion limit to enable the movement of viruses into adjacent cells (1, 2). Viral MPs use various transport strategies to reach PD and interact with several host factors to regulate movement processes (3–5). A well-studied viral MP, tobacco mosaic virus (TMV) MP, recruits cytoskeleton components (6–9), host factors involved in the intra- and intercellular transport (10–12), and cellular membranes (13). Unlike TMV MP, substantially less host components have been reported for tubule-forming MPs [e.g., grapevine fanleaf virus (GFLV), cauliflower mosaic virus (CaMV), and cowpea mosaic virus (CPMV)] (14–20). Many functionally and structurally diverse proviral host factors, particularly in the plant endomembrane system, remain to be elucidated.
The plant endomembrane system has unique players controlling trafficking pathways to modulate plant-specific cellular functions and stress responses (21, 22). The trans-Golgi network (TGN) acts as an early endosome (EE) where the major sorting processes (i.e., secretion, endocytosis, recycling, and vacuolar targeting) are orchestrated (23). Endocytosed cargoes are received by the TGN, and these cargoes are either returned to the plasma membrane or sent to multivesicular bodies (MVBs)/prevacuolar compartments (PVCs), which in plant cells serve as late endosomes (LEs). Heterotetrameric adaptor protein (AP) complexes, which consist of two large subunits (γ/α/δ/ϵ/ζ and β), a medium subunit (μ) and a small subunit (σ), are key components of protein sorting in endocytic and post-Golgi secretory pathways (24). AP-dependent protein sorting is mediated most commonly by the recognition of sorting signals, tyrosine (Y), YXXØ and dileucine (LL), [D/E]XXXL[L/I] motifs (where Ø is a bulky hydrophobic residue and X any amino acid) on cargo proteins. In plants, one of the most studied sorting signals is the Y motif. Several cell surface receptors, receptor-like kinases, and receptor-like proteins (25), transporters (26), and vacuolar sorting receptors (27–29) carry Y motifs. There are, however, only a few reports showing the recognition of these signals by AP complexes (27–32). LL motifs in plants are far less studied.
MPs of several viruses contain putative Y and LL motifs. However, the role of these sorting motifs in the targeting of viral MPs to PD is still largely unknown. The GFLV MP contains putative Y and LL motifs, which are also found in MPs of several other nepoviruses (14). The conserved YQDLN motif, which conforms to the Y motif, of the triple gene block protein TGB3 of poa semilatent virus (PSLV) is involved in targeting of the protein to the cell periphery (33). Potato mop-top virus (PMTV) TGB3 also carries the same motif, and the disruption of this motif by introducing mutations impairs the localization of PMTV TGB3 to the ER and motile granules, and the targeting of PMTV TGB3 to PD (34, 35). Moreover, three functional Y motifs (i.e., YLPL, YGKF, and YPKF) of CaMV MP govern the endosomal localization of CaMV MP and thus its peripheral presence (16).
Ourmia melon virus (OuMV; Ourmiavirus) was first discovered in Iran, where it is widely spread in cultivations of melon exhibiting mosaic and chlorotic spots and producing smaller fruits (36, 37). The mechanism of natural plant-to-plant transmission has yet to be elucidated as no biological vector has been identified so far. However, an extensive number of dicotyledonous plants, can be infected by OuMV through mechanical transmission (36, 38). OuMV harbors one of the simplest multipartite viral genomes organized as three positive sense, single-stranded, mono-cistronic RNA segments, each encoding for the viral RNA-dependent RNA polymerase (RdRp), MP, and coat protein (CP), respectively (36, 38, 39). Phylogenetic analyses suggest that OuMV progenitor could have originated from a reassortment event between a virus of a plant-pathogenic fungus and a plant-infecting virus (e.g., tombusvirus) (39, 40).
The MP of OuMV forms tubules across the cell wall of epidermal cells of OuMV-infected Nicotiana benthamiana; these tubules contain virions (39). Our previous studies showed OuMV-MP labeled protruding tubular/punctate structures in N. benthamiana and A. thaliana and puncta between neighboring cells co-localizing with callose deposits at PD (41, 42). None of the studies conducted so far points to a role of the OuMV MP in silencing (43). Exploring the role of conserved amino acids of MPs belonging to the 30KDa family led to the identification of three residues at positions 98, 150, and 169 required for targeting of OuMV MP, tubule formation in protoplasts, and possibly for the correct conformation of OuMV MP (42). Despite the available information, the specific molecular features of the movement of species in the genus Ourmiavirus are poorly understood today. Even though OuMV MP is currently categorized in the tubule-forming class, it is still unknown if it uses a novel mixed-movement model. Because of the unique features of OuMV MP and its commonalities with other 30KDa MPs, OuMV MP could interact with host factors and compartments used by other viral MP as well as with novel ones in infected cells. It is, however, still unknown which intracellular pathways OuMV MP uses to facilitate the cell-to-cell movement of virions. OuMV MP contains two potential internalization and trafficking motifs, one putative Y motif, 88YDKV, and one putative LL motif, 59DPIALI. Thus, we hypothesize that the OuMV MP would hijack endosomal pathways as the tubule forming MP of CaMV since the two movement proteins share sorting motifs. In this study, we report the involvement of OuMV MP in post-Golgi trafficking pathways. We characterized the individual function of each motif in relation to these pathways by introducing glycine substitutions into critical residues of these motifs and examining the localization of mutants by confocal microscopy. Moreover, we identified host factors potentially recruited by OuMV MP to reach the cell periphery.
Results
OuMV MP is associated with vesicle trafficking and lipid binding proteins
OuMV MP-interacting proteins were identified by using purified recombinant MBP-MP as bait to isolate interacting proteins from Arabidopsis leaf extracts and subjecting co-immunoprecipitated proteins to LC-MS/MS analysis. After removing non-specific interacting proteins (MBP-binding proteins), a total of 55 unique ‘detergent-soluble’ and 146 ‘soluble’ OuMV MP-interacting were discovered. Although ribosomal proteins, elongation factors, and chloroplast-related proteins could be functionally relevant, especially in the context of viral replication, these proteins were further removed from the analysis to focus on the transport of OuMV MP and the pathways it regulates. Among these candidates, 12 ‘detergent-soluble’ proteins (Table S1) and 35 ‘soluble’ proteins (Table S2) were selected as candidate interacting partners of OuMV MP, representing diverse pathways that OuMV MP could potentially target, including membrane trafficking, calcium signaling, transport across the plasma membrane, protein folding and homeostasis, proteolysis, and metabolism. Of these, nine host proteins are classified in the vesicle trafficking, including components of AP complexes, and three in the lipid binding, suggesting that the MP could associate with the plant endomembrane system. To further elucidate the membrane components OuMV MP could target in the plant, we screened various phospholipid candidates using a protein lipid overlay assay. OuMV MP exhibited affinity specifically for phosphatidylinositol 3-phosphate (Figure S1).
OuMV MP is trafficked through post-Golgi transport pathways
We previously investigated the subcellular localization of a OuMV GFP : MPwt fusion in N. benthamiana leaf epidermal cells, and demonstrated the presence of fluorescent foci at the cell surface, co-localized with PD (41, 42). Further investigation of the localization of GFP : MPwt, expressed together with the RdRp and CP, revealed that GFP : MPwt was associated with peripheral mobile structures (Movie S1). To examine whether OuMV MP localizes to endosomal compartments, we used an endocytic marker, FM4-64, which has been shown to follow endocytic pathways in plants (44–46). GFP : MPwt-marked puncta appeared to colocalize with FM4-64 in N. benthamiana protoplasts (Figure 1A). To identify the nature of the endosomal compartments in which the MP resides, we transiently co-expressed mCherry-fused organelle marker proteins (47), VTI12 (a TGN marker) (48), and RHA1 (a MVB/PVC marker) (49), with GFP : MPwt along with RdRp and CP in N. benthamiana leaf epidermal cells. We also applied two well-known inhibitors of vesicle trafficking, brefeldin A (BFA; an ARF GEF inhibitor) and Wortmannin (Wm; a phosphatidylinositol-3 and -4 kinase inhibitor), to these cells to dissect the pathways through which MP is trafficked.
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Figure 1 OuMV MP localizes to the TGN/EE, but not to the MVB/PVC or Golgi. (A) N. benthamiana protoplasts expressing OuMV GFP : MPwt (green) with RdRp and CP. Protoplasts were stained with FM4-64, an endocytic tracer (magenta). A selected region (white rectangle) was analyzed to show the colocalization of MP-labeled punctate structures (arrowheads) with FM4-64. The extent of colocalization was quantified using the PSC plugin in ImageJ. rp, linear Pearson correlation coefficient; rs, nonlinear Spearman’s rank correlation coefficient. Images represent single optical planes. Scale bars, 15 μm, 10 μm (magnified region). (B–D) N. benthamiana epidermal cells expressing GFP : MPwt (green) with RdRp and CP, and organelle markers (magenta), (B) mCherry : VTI12 (TGN/EE), (C) mCherry : RHA1 (MVB/PVC) and (D) Man49:mCherry (Golgi). Cells were treated with 10 μM BFA (B, D, bottom panel) or 33 μM wortmannin (C, bottom panel) for 1h. White arrowheads indicate colocalized spots, green and magenta arrowheads denote individual GFP : MPwt and mCherry : VTI12 spots, respectively. Images were taken at 66 hpi. Panels represent maximum intensity Z projections. Selected spots denoted by letters (B–D) and the signal along the line (D, bottom panel) were analyzed to show the intensity profile generated by ImageJ. Scale bars, 5 μm.
mCherry : VTI12 showed the expected punctate pattern of TGN that overlapped with GFP : MPwt (Figure 1B), suggesting that MP is recruited to TGN. It has been previously reported that 5-10 μM concentrations of BFA promote dissociation of TGN into small vesicular compartments in tobacco BY-2 cells (50). Hence, we treated N. benthamiana leaves expressing both mCherry : VTI12 and GFP : MPwt with 10 µM BFA. Upon BFA treatment, both mCherry : VTI12 and GFP : MPwt dispersed into the cytoplasm. While GFP : MPwt dissociated into smaller punctate structures, some of these structures did not completely co-localize with the TGN marker (Figure 1B, lower panel). When we increased the concentration of BFA to 50 μM, we observed BFA-induced GFP : MPwt aggregates in N. benthamiana epidermal cells. We hypothesize that the formation of these aggregates was associated with the toxicity of BFA rather than direct effect of BFA on the trafficking pathways, and we used the lower concentration in all the further experiments.
To assess whether OuMV MP enters a vacuolar trafficking pathway, we investigated the co-localization of GFP : MPwt with mCherry : RHA1. GFP : MP-labeled intracellular compartments did not co-localize with RHA1-positive MVBs/PVCs (Figure 1C, upper panel). Treatment with 33 μM Wm, which inhibits protein sorting to the vacuole by inducing homotypic fusion and enlargement of MVBs (51, 52), resulted in RHA1-positive MVBs/PVCs that appeared as ring-like structures (Figure 1C, lower panel). These structures were not, however, labeled by GFP : MP (Figure 1C, lower panel), indicating that MP does not shuttle through the MVB/PVC to the vacuole transport pathway. Unexpectedly, we also observed that the punctate signal of GFP : MP was shifted more toward a diffuse cytoplasmic pattern upon Wm treatment.
Since GFP : MPwt associated with the TGN, we further explored whether the MP puncta were also associated with Golgi bodies by transiently co-expressing GFP : MPwt with Man49:mCherry, a cis-Golgi marker (53). As expected, Man49:mCherry displayed a spot-like signal typical of Golgi bodies with a weak signal from the ER; however, the spot-like Golgi signal did not overlap with the punctate signal of GFP : MPwt, indicating that GFP : MPwt structures are distinct from Golgi bodies (Figure 1D, upper panel) and exclusive to the TGN. We also applied 10 μM BFA to N. benthamiana leaves expressing the Golgi marker and GFP : MPwt to further test whether MP sorts in the conventional secretory pathway. In tobacco, BFA inhibits the secretory pathway and causes redistribution of Golgi bodies into ER (54, 55). Indeed, treatment with 10 μM BFA caused the Golgi marker to relocate to ER (Figure 1D, lower panel). While GFP : MP showed a dispersed pattern similar to that observed with the TGN marker upon BFA treatment (Figure 1B, lower panel), this pattern did not co-localize with the Golgi marker, suggesting that GFP : MPwt does not enter the ER-to-Golgi secretory pathway.
OuMV MP carries Y and LL motifs, and substitutions into these motifs result in the loss of OuMV infectivity and reduced viral replication
OuMV MP carries one putative Y motif, YDKV (88–91), and one putative LL motif, DPIALI (56–61). To determine whether the Y and LL motifs in OuMV MP are functional, we substituted critical residues of these motifs with glycine and generated five mutants of wild-type MP (MPwt) by site-directed mutagenesis: Y (GDKG), Y/G (GDKV), D (GPIAGG), D/G (GPIALI) and LI/GG (DPIAGG), as shown in Figure 2A. To address possible roles of Y and LL motifs in infectivity of OuMV, the ability of mutants to infect N. benthamiana and Arabidopsis (Arabidopsis thaliana) was examined using OuMV agroclones carrying the desired mutations. Four out of five OuMV mutants carrying MP_Y, MP_Y/G, MP_D, or MP_LI/GG showed no visible symptoms in local (3 dpi) and systemic (14 dpi) leaves of N. benthamiana and Arabidopsis, whereas the OuMV carrying MPwt displayed typical symptoms of leaf chlorosis, curling, and stunted growth (Figures 2B, C). In Arabidopsis, MP_D/G carrying plants exhibited stunted growth as MPwt, but chlorosis and leaf curling were not observed (Figure 2B, right panel). Unlike in Arabidopsis, mutant MP_D/G occasionally caused mosaic symptoms in systemic leaves of N. benthamiana, but the overall symptoms were milder than upon infection with wild-type OuMV (Figure 2C; Figure S2). Symptoms of OuMV carrying MPwt or MP_D/G in N. benthamiana appeared as early as 5 dpi (Figure S2) and remained visible during the experimental time frame (14 dpi) (Figure 2C).
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Figure 2 Loss of infectivity and reduced viral replication of OuMV carrying Y and LL motif MP mutants. (A) Schematic representation of constructs used in this study. Boxes indicate Y and LL motifs, and mutated sites are shown in pink. The illustration was created with BioRender.com. (B, C) Viral symptoms on Arabidopsis (B) and N. benthamiana (C) agroinfiltrated with pGC-RNA1 (RdRp), pGC-RNA3 (CP), and pGC-RNA2 (MP) with indicated mutations, or sterile water (mock) at 14 dpi. White asterisks denote leaves shown in close-up images. Black arrowheads indicate mosaic symptoms on N. benthamiana infected with OuMV carrying MP_D/G. The background was removed from the images. (D) Western blot analysis of agroinfiltrated leaves at 3 dpi (bottom panel) and upper uninoculated leaves at 14 dpi (top panel). CBB: Coomassie Brilliant Blue, loading control. (E) Replication assay of OuMV carrying Y and LL motif MP mutants at 48 hpi. Two leaf spots from three leaves were pooled for each sample at 24 hpi and 48 hpi. The qPCR was performed in duplicates. The 2-ΔΔCt method was used to assess fold changes in replication of wild-type and mutants. The data was normalized first using 18S ribosomal RNA, and then a wild-type control at 24 hpi showing the lowest expression. Box plot represents the relative expression of RNA1 (n=4). The statistical analysis was performed on ΔΔCt values using Student’s t-test (unpaired, two-tailed).
The presence of MP mutants in upper uninoculated leaves of N. benthamiana at 14 dpi was tested by western blot analysis; only MPwt and MP_D/G could be detected (Figure 2D, top panel). To test whether the impaired systemic movement of OuMV mutants was due to a lack of expression of MP mutants at the agroinfiltration sites, we performed western blot analysis of N. benthamiana leaf extracts for all constructs at 3 dpi, and all MP variants could be detected (Figure 2D, bottom panel). Virion accumulation was also determined in upper uninoculated leaves at 14 dpi by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using α-CP antiserum. All mutants, except the one carrying MP_D/G, failed to move systemically in both N. benthamiana and Arabidopsis (Table 1). We also analyzed the coding sequence of MP in OuMV mutants by RT-PCR and sequencing of the PCR product to determine whether mutations in Y and LL motifs were reversed, or whether additional mutations were introduced during infection assays. The reversion of MP_D to wild-type was observed only in one Arabidopsis plant (Table 1), where symptoms became systemic.
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Table 1 Infectivity assays of OuMV carrying MPwt or MP mutants in N. benthamiana and Arabidopsisa.
Next, we assessed if OuMV carrying MP mutants could be inhibited in virus replication. Briefly, we co-infiltrated each MP mutant or MPwt along with RdRp, CP, and pBin61-GFP and collected samples from the infiltrated leaves at 24 hpi and 48 hpi. GFP signal was monitored at 36 hpi to confirm that all plant cells at the infiltrated areas were infected (Figure S3). Quantification of RNA1 (RdRp) as a proxy for replication was performed by qRT-PCR in all samples. At 24 hpi, RNA1 levels of wild-type OuMV were comparable to that of MP mutants (Figure S4). While all mutants were able to replicate at high levels at 48 hpi, we observed a substantial decrease in the replication of the mutants compared to corresponding wild-type controls (Figure 2E). This result suggests that mutations in the OuMV MP Y and LL motifs have also an effect in virus replication.
Given that the μ subunit of Arabidopsis AP2 (AP2M) plays a direct role in the intracellular trafficking of CaMV MP (16), we also investigated the possible role of AP2M in the transport of OuMV MP by infecting Arabidopsis Col-0 (wt), AP2M knockout mutant ap2m-1 and AP2M-YFP complementation plants with OuMV by mechanical inoculation. There were no obvious differences in symptoms or changes in the number of infected plants among the ap2m-1 mutant, the complementation line and wt at 14 dpi, indicating that the absence of AP2M does not impair systemic infection of OuMV (Table S3).
Y and LL motif MP mutants display changes in subcellular localization over time
We next examined whether Y and LL motif MP mutants display different intracellular localization patterns over time (Figure S5). To characterize the subcellular distribution of MP mutants, we transiently expressed GFP : MPwt (Figure S5A), as a control, and GFP : MP mutants (Figures S5B-F) with RdRp and CP in N. benthamiana epidermal cells. GFP-tagged Y motif MP mutants, GFP : MP_Y and GFP : MP_Y/G, appeared to be distributed in the cytoplasm at 36 hpi and 48 hpi (Figures S5B, C, left panels) without any noticeable change at 72 hpi (Figures S5B, C, right panel). Y motif mutants accumulated in transvacuolar strands and in patches at the cell cortex. Puncta that were consistently observed in GFP : MPwt (Movie S2) were no longer apparent in GFP : MP_Y and GFP : MP_Y/G (Movie S3). Occasionally, a few punctate structures were observed in GFP : MP_Y/G; however, these structures were either stationary or had rather restricted movement (Movie S3). GFP-tagged LL motif MP mutants had a cytoplasmic localization at earlier time points (Figures S5D-F), with peripheral and cytoplasmic puncta readily observable at 60 hpi, similar to GFP : MPwt (Figures S5D-F and Movie S4).
Mutations in the Y but not in the LL motif impair plasma membrane targeting of MP
To investigate whether Y and LL motif MP mutants are targeted to the plasma membrane, we stained N. benthamiana protoplasts expressing GFP : MPwt or GFP : MP mutants with FM4-64, a plasma membrane marker. GFP : MPwt showed a continuous signal at the cell periphery and this signal strongly overlapped with FM4-64 (Figure 3A). The peripheral signal of GFP : MP_Y and GFP : MP_Y/G often did not co-localize with FM4-64 (Figures 3B, C). A similar pattern was observed in N. benthamiana epidermal cells expressing GFP : MP_Y or GFP : MP_Y/G, where the peak of FM4-64 often positioned in between two peaks of the MP mutants, suggesting that plasma membrane localization of GFP : MP_Y and GFP : MP_Y/G followed a different pattern than GFP : MPwt (Figure S6). Moreover, both mutants exhibited an intense and diffuse cytoplasmic signal. The peripheral signal of GFP : MP_D/G mostly overlapped with FM4-64 (Figure 3D). The movement deficient mutant GFP : MP_D also displayed a peripheral signal that co-localized with FM4-64; however, the GFP signal was not evenly distributed along the plasma membrane and accumulated at specific sites (Figure 3E). GFP : MP_LI/GG, on the other hand, had regions where the signal is either continuous or puncta (Figure 3F).
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Figure 3 Plasma membrane localization of MP mutants. N. benthamiana protoplasts expressing (A) GFP : MPwt or GFP : MP mutants, (B) GFP : MP_Y, (C) GFP : MP_Y/G, (D) GFP : MP_D/G, (E) GFP : MP_D and (F) GFP : MP_LI/GG along with RdRp and CP. Protoplasts were stained with FM4-64, a plasma membrane marker (magenta). Selected regions (white rectangles) showing the intensity profile of the diagonal line were generated by ImageJ. Images represent single optical planes. Scale bar, 5 μm (magnified region).
To investigate these observations, N. benthamiana epidermal cells expressing GFP : MPwt or mutants were subjected to plasmolysis (Figure 4). While GFP : MPwt was associated with Hechtian strands that attach the plasma membrane to the cell wall (Figure 4A), GFP : MP_Y and GFP : MP_Y/G did not exhibit the same pattern as GFP-marked strands were absent from the space between the protoplast and the cell wall (Figures 4B, C). Hechtian strands were, however, observable in GFP : MP_D, GFP : MP_D/G, and GFP : MP_LI/GG, similar to GFP : MPwt (Figures 4D-F). Plasma membrane localization of GFP : MP_D and GFP : MP_LI/GG suggests that mutations of the LL motif do not impair plasma membrane targeting of MP but alter uniform distribution of MP along the plasma membrane.
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Figure 4 LL motif MP mutants are targeted to the plasma membrane. N. benthamiana epidermal cells expressing GFP : MPwt (A) or GFP : MP mutants, GFP : MP_Y (B), GFP : MP_Y/G (C), GFP : MP_D/G (D), GFP : MP_D (E), and GFP : MP_LI/GG (F) along with RdRp and CP. were plasmolyzed with 1M mannitol. Arrows show the retracted plasma membrane and asterisks denote Hechtian strands. Images were taken at 62-66 hpi and represent single optical planes. Scale bar, 10 μm.
Movement deficient LL motif MP mutants fail to localize to PD
The punctate localization of the two movement-deficient LL motif MP mutants, MP_D and MP_LI/GG, along the cell periphery raises the question of whether these punctate structures are associated with PD. We examined the targeting of MP mutants to PD by staining N. benthamiana epidermal cells expressing GFP : MPwt or MP mutants with aniline blue, a callose-specific dye. Consistent with our previous results (42), GFP : MPwt co-localized with callose deposits at PD (Figure 5A). GFP : MP_D/G, which is able to support movement, exhibited a similar localization pattern compared to GFP : MPwt, and the intensity profile across the PD strongly coaligned with that of aniline blue (Figure 5B). Although peripheral punctate structures marked by GFP : MP_D and GFP : MP_LI/GG did not predominantly localize to callose deposits (Figure 5C), GFP : MP_LI/GG labeled structures were in close proximity to PD (Figure 5D). The quantification of points labeled by GFP : MPwt or GFP : MP mutants and aniline blue revealed that some of GFP : MP_D and GFP : MP_LI/GG co-localize with callose deposits, but the number of co-localized signal was much lower than that of GFP : MPwt and GFP : MP_D/G (Figure 5E). The statistical analysis of co-localization revealed significant differences between GFP : MPwt or GFP : MP_D/G and movement deficient MP mutants, GFP : MP_D and GFP : MP_LI/GG (Figure 5F). In contrast, no significant difference between GFP : MPwt and GFP : MP_D/G was observed (Figure 5F). These results suggest that OuMV movement correlates with the ability of its movement protein to be targeted to PD. Results for all mutants are summarized in Table S4.
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Figure 5 Movement deficient LL motif MP mutants do not localize to callose deposits. N. benthamiana epidermal cells expressing (A) GFP : MPwt or GFP : MP mutants, (B) GFP : MP_D/G, (C) GFP : MP_D, and (D) GFP : MP_LI/GG (magenta) with RdRp and CP. Aniline blue was used to mark callose deposits (cyan). Selected regions (white rectangles) show the intensity profile of the diagonal line generated from images that were adjusted to a lower intensity by ImageJ. Images represent single optical planes. Scale bar, 20μm, 10 μm (magnified region). (E) The quantification of callose deposits (blue), punctate structures labeled by OuMV GFP : MPwt or GFP : MP mutants (magenta), and co-localized spots (gray) per area of view. The spots were counted using the ComDet v.0.4.1 plugin in ImageJ. At least six areas of view were examined (n≥6) and the total number of counted spots for each sample is more than 700. Stacked bar graph represents mean ± standard deviation (F) The statistical analysis of co-localized spots in (E). Bar graph represents PD-localized OuMV GFP : MPwt or GFP : MP mutants per area of view (mean± standard deviation, n≥6). ***P < 0.001 by ANOVA, followed by Tukey’s HSD.
To understand the specific role of LL motif in the plasmodesmata targeting of OuMV MP, N-terminal domain truncated OuMV MP mutants retaining the LL motif, MP_84-first 84 amino acids of MP, and retaining both LL and Y motifs, MP_102-first 102 amino acids of MP, were generated based on predicted secondary structure of MP. We monitored the targeting of truncated MP as described above. Both mutants were, however, unable to reach PD (Figure 6), indicating that the presence of the LL motif in a truncated MP variant is not sufficient to support plasmodesmata targeting of MP, and the C-terminal region of MP is also involved in this process. Moreover, MP_102 had noticeably lower expression levels, close to the background signal, compared to MP_84.
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Figure 6 Truncated OuMV MP mutants retaining sorting motifs fail to localize to callose deposits. N. benthamiana epidermal cells expressing (A) GFP : MP_84, (B) GFP : MP_102 (magenta) with RdRP and CP. Aniline blue was used to mark callose deposits (cyan). Images represent single optical planes. Scale bar, 15μm, 10 μm (magnified region). (C) The quantification of callose deposits (blue), spots labeled by OuMV GFP : MP truncated mutants and co-localized spots (gray) per area of view. The spots were counted using the ComDet plugin in ImageJ. Four areas of view were examined (n=4) and the total number of counted spots for each sample is more than 600. Stacked bar graph represents mean ± standard deviation.
Discussion
In this study, we investigated the intracellular trafficking of OuMV MP in the context of post-Golgi transport routes and the function of Y and LL sorting motifs. Despite the complexity of plant protein sorting arising from the fact that TGN acts as the main sorting hub for Golgi-derived cargoes and internalized cargoes from the plasma membrane, we were able to partially unravel the trafficking routes that OuMV MP hijacks by using organelle markers in combination with inhibitors of vesicle trafficking. TGN localization of OuMV MP, together with its co-localization with FM4-64 stained compartments, demonstrates that OuMV MP traffics in endosomal pathways (Figures 1A, B). Co-localization of endosomal compartments and MPs of other viruses has been reported before (16, 34); however, this is the first study showing that a viral MP resides in the TGN, extending knowledge of the involvement of post-Golgi trafficking pathways in virus movement. We also showed that the canonical secretory pathway is not involved in the transport of OuMV MP since the MP did not co-localize with the Golgi in the absence or presence of BFA (Figure 1D). Moreover, the redistribution of OuMV MP into the same smaller vesicles as the TGN marker upon low dose BFA treatment suggests that MP exhibits BFA sensitivity only at the TGN, but not at the Golgi. Dependence on the canonical secretory pathway varies between plant viruses (4). For example, whereas the secretory pathway is not involved in targeting of the CPMV MP to the cell periphery (62), a functional secretory pathway is necessary for the correct localization of the GFLV MP (14). In addition, OuMV MP was not associated with MVBs/PVCs in the absence or presence of Wm (Figure 1C), confirming that MP traffics in the first stages of vesicular trafficking, but not in MVB/PVC-to-vacuole pathway, in contrast to CaMV MP (16) and PTMV TGB2 (34), which co-localize with ARA7-positive MVBs/PVCs. Although OuMV MP showed sensitivity to Wm, this response was different from that of MVBs/PVCs, probably due to the effect of Wm on endocytosis. Based on the finding that OuMV MP localizes to the TGN and does not enter either the canonical secretory or MVB/PVC-to-vacuole pathway, we hypothesize that MP constitutively cycles between the plasma membrane and TGN. Although a similar recycling mechanism has been proposed for CaMV MP (16), the TGN localization of CaMV MP was not explored.
Glycine substitutions in the Y motif altered the intracellular distribution of MP. A key question emerging from this observation is whether the Y motif in OuMV MP is a functional internalization motif. Y motifs are recognized by μ-subunits of APs 1-4 (24, 63, 64). In plants, clathrin-mediated endocytosis (CME) of plasma membrane proteins is achieved by the recognition of Y motifs in their cytoplasmic domains by AP2M (56–59), and it has been previously shown that CaMV MP is able to bind Arabidopsis AP2M through three Y motifs (16). While mutations in these motifs do not affect targeting of the protein to the plasma membrane, its internalization relies on at least one functional Y motif. Unlike CaMV, mutations in the Y motif retained OuMV MP in the cytoplasm. Additionally, we did not observe any changes in the infectivity of OuMV in the absence of AP2M in Arabidopsis (Table S3). It is, however, known that AP2M is not the sole regulator of CME in plants; a CME adaptor complex, TPLATE complex (TPC), is involved in the initiation of CME and is thus crucial for recruitment of clathrin and AP2 (60). Moreover, AP2 subunits are considered to form partially active complexes in the absence of single subunits (61). Hence, other AP2 subunits and/or TPC may be important for internalization of OuMV MP, and in turn for infectivity of OuMV. Indeed, the identification of AP2α as a candidate interactor (Table S2) suggests that the internalization of OuMV MP may still occur via an AP2-dependent mechanism. Alternatively, Y motif may have other functions such as maintaining correct protein conformation. Glycine substitutions into the Y motif of OuMV MP may induce conformational changes, leading to masking of the plasma membrane targeting signal. In PSLV and PMTV, the Y motif is required for the delivery of viral proteins to the cell periphery and PD, but this motif is important for conformation of the protein, rather than its internalization (35). We previously identified three amino acid residues (98, 150 and 169) within the core region of OuMV MP (63–206), which are responsible for localization of MP at the cell periphery, and these residues are possibly involved in the correct folding/function of OuMV MP (42). The Y motif of OuMV MP is located in the core region of the MP, suggesting that it may have a structural role, similar to the one seen for PSLV TGB3 and PMTV TGB3. Moreover, The Y motif of OuMV MP may be important for recruiting viral and cellular factors that are necessary for intracellular targeting as the YQDLN motif in the TGB3 of PSLV plays a crucial role in its intracellular transport, facilitating the formation of higher-order complexes (65).
OuMV MP has the classical [D/E]XXXL[L/I] sorting motif in which an aspartic acid is located at the position 1. Replacing the aspartic acid with glycine in DPAILI does not affect the localization of the MP and the resulting mutant behaves similar to the wild-type virus (Figure S5E; Figures 3, 4D, 5B). Conversely, substitutions of leucine and isoleucine with a pair of glycine residues impair the transport of the OuMV MP to PD. In plants, the LL motif is recognized by AP complexes (66, 67). The LL motif of the tonoplast-localized ion transporter VTI1, EKQTLL, exhibits affinity for AP1γ1/2 and σ1/2 subunits (66). Notably, AP1γ co-purified with OuMV MP (Table S2), thus strengthening the possibility of AP1γ as an interacting partner of OuMV MP. Intriguingly, AP2β has been recently shown to interact with turnip mosaic virus (TuMV) proteins (i.e., 6K2, VPg, CI, and CP), which harbor putative LL motifs, implicating a potential role of the LL motif in the endocytosis during viral infection (68).
Previous studies have shown that viral proteins traffic in endocytic and vacuolar pathways (11, 16, 34, 68, 69). Our results support the hypothesis that plant viral proteins take advantage of post-Golgi trafficking pathways to facilitate cell-to-cell movement and provide new insights into these pathways. Indeed, OuMV MP takes advantage of endosomal pathways as CaMV MP but appears to use a different mechanism. Based on our findings, we propose the following model (Figure 7): OuMV MP is targeted to the plasma membrane through a mechanism that is independent of the conventional secretory pathway. While MPs of some viruses reach the plasma membrane and PD through the ER (4, 70), we do not have evidence at this point that OuMV MP follows this model. Once OuMV MP reaches the plasma membrane, it is endocytosed, sorted into the TGN, and then recycled back to the plasma membrane, bypassing the MVB/PVC-to-vacuole pathway, to reach PD. The retrieval of MP from the plasma membrane does not appear to depend on the Y motif. On the other hand, the PD targeting of MP relies on the LL motif, potentially through its function in endocytic pathways. The precise molecular mechanism of MP targeting is, however, still unknown. In fact, the N-terminal region of MP retaining the LL motif does not act as a PD-targeting signal (Figure 6), as opposed to the TMV MP, in which the first 50 amino acid residues were characterized as a PD localization signal (71). Moreover, the C-terminus of OuMV has a predicted disordered region with many potential phosphorylation sites that may be important for its regulation and interaction with host proteins. The model presented in this study requires further examination to understand the mechanistic details of the targeting of OuMV MP to PD. Movement proteins are often associated with the sites of viral replication. Thus, it is extremely hard to untangle if a disturbance in viral replication, as seen with our mutants, is due to mislocalization of the viral MP from the replication site or to modifications of the sites of replication. While we do not have experimental evidence of the co-localization of the OuMV MP and RdRp, many studies suggest that the two proteins target the same replication complexes (72). Although a role of OuMV MP in evasion of plant defenses cannot be ruled out and was not the object of this study, prior work does not support its function as silencing suppressor (43). Notably, our characterization of OuMV MP-targeted pathways partially reveals missing molecular and cellular components of OuMV movement and virus-host interactions that could be used as target for antiviral strategies.
![www.frontiersin.org](https://www.frontiersin.org/files/Articles/934011/fviro-02-934011-HTML/image_m/fviro-02-934011-g007.jpg)
Figure 7 Working model for intracellular trafficking of OuMV MP. The MP (green) is targeted to the plasma membrane via an unknown mechanism. It is retrieved from the plasma membrane (dark gray) and transported to the TGN (purple). From the TGN, the MP is recycled back to the plasma membrane (pink). The Y motif possibly acts as a structural motif rather than a functional internalization motif, whereas the LL motif is required for correct plasmodesmata targeting of MP. TGN/EE, trans-Golgi network/early endosome; PM, plasma membrane; PD, plasmodesmata; CW, cell wall. The model was created with BioRender.com.
Materials and methods
Plant material and growth conditions
N. benthamiana plants were grown in a growth chamber set at 25°C with a 16 h photoperiod and used at 4-5 weeks old. Arabidopsis thaliana seeds were surface sterilized using 50% bleach solution for 5 minutes, rinsed five times with sterile distilled water and plated on ½ MS plates containing 1% agar. Ten-day old seedlings were transferred to soil, grown in a growth chamber at 22°C, 60% relative humidity, and at a light intensity of 120 µmol/m2s1 at a 10 h photoperiod, and used when 4-5 weeks old.
Arabidopsis thaliana ecotype Col-0 was used for infectivity assays. The adaptor protein complex 2 medium subunit (AP2M) knockout mutant ap2m-1 (SALK_083693) and AP2M-YFP complementation line (73) were provided by Dr. Ying Gu (The Pennsylvania State University, University Park, PA, USA) and genotyped for homozygosity using the primers in Table S5.
Plasmid constructions and Agrobacterium-mediated transient expression in plant leaves
The OuMV infectious clone, consisting of the three plasmids pGC-RNA1 (RdRp), pGC-RNA2 (MP) and pGC-RNA3 (CP), and GFP : MP plasmid has been previously described (41, 42). Mutations were inserted into MP and GFP : MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S5.
The Golgi marker, Man49:mCherry (CD3-967) (53), the TGN marker, mCherry : VTI12 (CD3-781541) (47), and the MVB/PVC marker, mCherry : RHA1 (CD3-781597) (47) were obtained from ABRC (http://www.arabidopsis.org/). The fragments of Ubq10pro::mCherry::VTI12 and Ubq10pro:mCherry::RHA1 were amplified from the original plasmids using the primers in Table S5, and inserted into XhoI/SpeI-digested pORE O2 (CD3-921) (74). All plasmids were transformed into A. tumefaciens strain C58C1.
A. tumefaciens cultures carrying the desired plasmids were grown in a shaking incubator at 28°C in 2xYT medium supplemented with 50 µg/ml kanamycin and 5 µg/mL tetracycline. Cultures were pelleted at an optical density at 600 nm (OD600) of 1.0 and resuspended in agroinfiltration buffer (10 mM MES, 10 mM MgCl2 and 100 µM acetosyringone). Bacterial suspensions were generated by mixing the desired combinations at a final optical density at 600 nm (OD600) of 0.2 and 0.05 for GFP : MP/RdRp/CP and the organelle markers, respectively. The mixtures were incubated at room temperature for 2 h in the dark and infiltrated on the abaxial side of fully expanded leaves using a needleless syringe.
Infectivity assays, ELISA, and RT-PCR
Viral infections were started either from agroinfiltration of a mixture of Agrobacterium cultures carrying the three plasmids mentioned above or by mechanical inoculation using sap obtained by grinding virus-infected fresh N. benthamiana tissue in 0.05 M phosphate buffer supplemented with carborundum and silica powders (42). Six N. benthamiana and 12 Arabidopsis plants were used for infectivity assays. Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) was performed as previously described (42). Leaves were sampled for ELISA testing at 14 dpi. Experiments were repeated three times.
RNA extraction and RT-PCR were performed as described in (42). Total RNA (100 ng) was used for RT-PCR, and full-length coding sequence of MP was amplified using primers listed in Table S5.
Replication assay
The effect of specific amino acid substitutions in MP on virus replication was measured by agroinfiltration of local leaves with pGC-RNA2 (MP) or mutants, together with pGC-RNA1 (RdRp), pGC-RNA3 (CP), and pBin61-GFP at an OD600 of 1.0. The GFP signal at the infiltrated sites was monitored at 36 hpi to confirm that agroinfiltrated areas were saturated. Two leaf spots from three leaves were pooled for each sample at 24 hpi and 48 hpi. The virus accumulation was measured by quantifying RNA1 by qPCR using primers listed in Table S5. The qPCR was performed in duplicates using iTaq™ Universal SYBR® Green Supermix (BioRad) and a CFX96 (BioRad), following the manufacturer’s instructions. The 2-ΔΔCT method was used to assess fold changes in replication of wild-type and mutants. The data was normalized by first using 18S ribosomal RNA and then a wild-type control at 24 hpi showing the lowest expression. Four plants were used for qPCR analysis. Statistical analysis was performed using Student’s t-test.
Protoplast isolation from infected leaves
Protoplasts were prepared from leaves of N. benthamiana expressing GFP : MPwt or GFP : MP mutants (together with RdRp and CP) at 66 hpi. Leaf sections were cut into small fragments (~1 cm2) using a razor blade and incubated in the enzyme solution on a rotor shaker for 6 h at room temperature, as previously described (42).
Plasmolysis, inhibitor, and staining experiments
Plasmolysis experiments were carried out by treating N. benthamiana leaf sections expressing GFP : MP or mutants, together with RdRp and CP, with 1 M mannitol for 45 min. The leaf sections were imaged at 62 – 66 hpi.
N. benthamiana leaves were infiltrated with 10 μM BFA (stock: 10mg/ml in DMSO; MilliporeSigma) or 33 μM Wortmannin (stock: 10 mM in DMSO; MilliporeSigma). Equivalent amounts of DMSO were used as controls. Leaf samples were imaged after 1 h of the indicated treatment, as described below. N. benthamiana protoplasts and epidermal cells were stained with 10 μM (stock: 1 mM in water) FM4-64 (Thermo Fisher Scientific), an endocytic marker that labels plasma membrane and then endosomal compartments in a time-dependent manner, for 5-15 min. For staining callose deposits, N. benthamiana leaves were infiltrated with aniline blue (MilliporeSigma) at a final concentration of 0.1% (stock: 1% (v/w) in 50 mM potassium phosphate buffer, pH 9.0) and incubated for 30 min. All experiments were repeated at least twice.
Confocal microscopy and quantification
Leaf sections or protoplasts were mounted on a microscope slide (Thermo Fisher Scientific) covered with a #1.5 coverslip (Corning). For confocal microscopy, images were acquired with a Zeiss Cell Observer SD microscope equipped with a Yokogawa CSU-X1 spinning disk head and Plan-Apochromat 63X/NA 1.4 and 40X/NA 1.3, and an Olympus Fluoview (FV) 1000 microscope with a Plan-Apochromat 40X/NA 1.15 water immersion and 60X/NA 1.4 oil immersion objectives. A 543-nm excitation laser line and an emission bandpass filter 585 – 660 nm (Olympus FV1000) were used for imaging of FM-4-64. Aniline blue was imaged using 405-nm excitation laser with an emission bandpass filter 425 – 475 nm (Olympus FV1000 and Zeiss Cell Observer SD). Z-stacks of optical sections were collected at 0.2 μm intervals for time-dependent subcellular localization experiments and at 0.5 μm intervals for the rest of the experiments. Images were analyzed and processed using ImageJ (http://imagej.nih.gov/ij). The background was subtracted, and the signal intensity was adjusted. Corresponding images were later processed by Adobe Photoshop CC 2017. All images were assembled in Adobe Illustrator CC 2022.
Callose deposits were quantified using the ComDet v.0.4.1 plugin in ImageJ. The detection was performed by setting spot size to 2 pixels. For co-localization experiments, maximum distance between spots was adjusted to 2 pixels. Statistical analysis was performed using ANOVA.
Protein purification
The coding sequence of OuMV MP was cloned into in pMAL-c4x (New England Biolabs; kindly provided by Dr. Gitta Coaker (University of California-Davis, CA, USA), in frame with MBP. MBP-MP was expressed in BL21 (DE3), and the expression was induced with 0.3 mM IPTG for 2 h at 37° C. The pellets were resuspended with amylose column buffer (20 mM Tris-HCl pH7.5, 0.2 M NaCl, 1 mM EDTA) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) and sonicated five times for 10 sec. The soluble fraction of MBP-MP was applied to pre-equilibrated amylose agarose resin (New England Biolabs). After washing the column three times with amylose column buffer, MBP-MP was eluted with five fractions of amylose column buffer supplemented with 30 mM maltose. The fraction containing the highest amount of MBP-MP was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with Ultracel- 50kDa (MilliporeSigma) and stored in storage buffer (20 mM HEPES pH 7.5, 5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 20% glycerol).
Co-IP assay
One gram of five-weeks old Arabidopsis rosette leaves were homogenized in 2ml of cold IP buffer (20 mM HEPES pH 7.4, 100 mM NaCl, 5 mM MgCl2, 5% glycerol, 0.5% Triton X-100, and 1X Halt protease/phosphatase inhibitor cocktail, Thermo Scientific) using a pre-cooled mortar and pestle. The homogenate was first centrifuged at 1,000g for 10 min at 4°C, and then at 10,000g for 10 min at 4°C. For the second part of the study, 10 grams of five-weeks old Arabidopsis rosette leaves were homogenized in 15 ml of cold homogenization buffer (50 mM HEPES pH 7.4, 330 mM sucrose, 10% glycerol and 1X Halt protease/phosphatase inhibitor cocktail, Thermo Scientific) using a pre-cooled mortar and pestle and filtered through four layers of Miracloth. The homogenate was first centrifuged at 1,000g for 10 min at 4°C, and then at 10,000g for 10min at 4°C. The supernatant was centrifuged to pellet microsomal membranes at 100,000g for 1 h at 4°C. The supernatant was used for the assay. Five μg of MBP or MBP-MP were bound to MBP-Trap-A beads (ChromoTek) for 1 h at 4°C with rotation and washed three times with washing buffer (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA) and two times with IP buffer. MBP or MBP-MP bound beads were incubated with plant lysate for 2 h at 4°C with rotation. Samples were washed three times with an IP buffer with 0.2% Triton X-100 and twice with 50 mM (NH4)2CO3, pH 8.0 to remove the detergent.
On-bead trypsin digestion and LC-MS/MS
The spin columns were blocked with plugs and 100 μl of on-bead trypsin digestion buffer (50 mM (NH4)2CO3, 0.01% ProteaseMax (Promega), and 1 μg Trypsin (Promega) was added to the columns. The samples were digested for 1 h at 37°C with gentle shaking to allow beads to be mixed in the solution. The digested samples were collected in low-bind tubes (Eppendorf). The beads were washed twice with 50 μl of on-bead trypsin digestion wash buffer (50 mM (NH4)2CO3, 0.01% ProteaseMax, and 5 mM DTT). The samples were incubated for 30 min at 37°C. Five μl of 500 mM Iodoacetamide (IAA) were added to digested samples and the samples were incubated at 37°C overnight in the dark. Three μl of 1M DTT were added to the samples to quench IAA and incubated for 30 min at 37°C. Ten ul of 10% trifluoroacetic acid (TFA) were added to a final concentration of 0.5% to acidify the samples and stop the digestion. The samples were incubated for 5 min at room temperature. Samples were snap-frozen in liquid nitrogen and lyophilized. Lyophilized samples were sent to the Penn State College of Medicine’s Mass Spectrometry & Proteomics Core for protein identification. The samples were desalted with C18 stage tips and injected into an ABSciex TripleTOF 5600+ (Applied Biosystems). MS/MS data were analyzed using ProteinPilot software. Unused Score > 1.3 (95% confidence) was used as a cut-off threshold to identify proteins.
Protein lipid overlay assay
The phospholipid-binding analysis of the MBP-MP was performed using PIP Strips (P-6001; Echelon Biosciences). The PIP strips were blocked for 1 h at room temperature in Tris-buffered saline (TBS) with 4% non-fat milk and then incubated 1 h at room temperature in blocking buffer containing 3.5 µg/ml MBP-MP. The membranes were washed with TBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-MBP monoclonal antibody (New England Biolabs; 1:5000).
Protein extraction, SDS–PAGE, immunoblotting
Leaf sections were collected from agroinfiltrated leaves and upper uninoculated leaves and homogenized in a 2x SDS-PAGE buffer. Extracted proteins were separated in SDS polyacrylamide gels (4% stacking gel, 10% separation gel). The proteins were transferred to PVDF membranes (MilliporeSigma) for western blot analysis. OuMV MP was detected using a non-commercial anti-MP (A314) antiserum (1:1000) as described in (39), followed by incubation with an anti-rabbit HRP-conjugated secondary antibody (NXA931; MilliporeSigma) diluted at 1:20000 (v/v). SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) was used for detecting HRP activity.
Data availability statement
The data presented in the study are deposited in the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD035960. The dataset has been made public Project Webpage: http://www.ebi.ac.uk/pride/archive/projects/PXD035960.
Author contributions
NO and CR designed the study and wrote the manuscript. NO conducted experiments. NO, PM, and CR analyzed the data. CTA and MT helped with data interpretation. All authors reviewed the results and manuscript and approved the final version of the manuscript.
Funding
This work was supported by USDA HATCH Accession No: 1009992, Project No. PEN04604 to CR and a grant of Penn State College of Agricultural Sciences to NO.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fviro.2022.934011/full#supplementary-material
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Keywords: intracellular transport, vesicle trafficking pathway, endomembrane, dileucine motif, tyrosine motif, plant virus-host interaction, host factors
Citation: Ozber N, Margaria P, Anderson CT, Turina M and Rosa C (2022) Sorting motifs target the movement protein of ourmia melon virus to the trans-Golgi network and plasmodesmata. Front. Virol. 2:934011. doi: 10.3389/fviro.2022.934011
Received: 02 May 2022; Accepted: 25 August 2022;
Published: 16 September 2022.
Edited by:
Ioly Kotta-Loizou, Imperial College London, United KingdomReviewed by:
Katarzyna Otulak-Kozieł, Warsaw University of Life Sciences - SGGW, PolandLiying Sun, Northwest A&F University, China
Copyright © 2022 Ozber, Margaria, Anderson, Turina and Rosa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Natali Ozber, natali.ozber@ucalgary.ca
†Present addresses: Natali Ozber, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada
Paolo Margaria, Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Plant Virus Department, Braunschweig, Germany
‡Senior author