Helena T. Hogberg*
Lena Smirnova- Center for Alternatives to Animal Testing (CAAT), Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States
Human brain is undoubtedly the most complex organ in the body. Thus, it is difficult to develop adequate and at the same time human relevant test systems and models to cover the aspects of brain homeostasis and even more challenging to address brain development. Animal tests for Developmental Neurotoxicity (DNT) have been devised, but because of complex underlying mechanisms of neural development, and interspecies differences, there are many limitations of animal-based approaches. The high costs, high number of animals used per test and technical difficulties of these tests are prohibitive for routine DNT chemical screening. Therefore, many potential DNT chemicals remain unidentified. New approach methodologies (NAMs) are needed to change this. Experts in the field have recommended the use of a battery of human in vitro tests to be used for the initial prioritization of high-risk environmental chemicals for DNT testing. Microphysiological systems (MPS) of the brain mimic the in vivo counterpart in terms of cellular composition, recapitulation of regional architecture and functionality. These systems amendable to use in a DNT test battery with promising features such as (i) complexity, (ii) closer recapitulation of in vivo response and (iii) possibility to multiplex many assays in one test system, which can increase throughput and predictivity for human health. The resent progress in 3D brain MPS research, advantages, limitations and future perspectives are discussed in this review.
Introduction
Overview of Brain Microphysiological Systems
Microphysiological systems (MPS) have emerged over the last years and are representing new, more physiologically relevant cell cultures recapitulating organ architecture and functionality (Marx et al., 2020). The MPS can have different levels of complexity going from simpler spheroids to organoids, microfluidics and organs-on-chip (Figure 1). The term MPS in this review refers to any of these models with the focus on 3D brain models or brain organoids (Lancaster and Knoblich, 2014; Pasca et al., 2015; Di Lullo and Kriegstein, 2017; Koo et al., 2019; Shou et al., 2020; Sun et al., 2021). The main features of brain organoid cultures are representation of the in vivo brain in terms of cellular composition, recapitulation of regional architecture (e.g., cortical layers) and functionality (e.g., active synapses, electrical activity and myelination). The brain MPS are now broadly used to study neurological disorders, brain development and aging (Di Lullo and Kriegstein, 2017; Koo et al., 2019; Shou et al., 2020; Sun et al., 2021). Although, as all in vitro models, the MPS also have limitations. Researchers are working on advancing the MPS (second generation MPS) (Marx, 2020; Marx et al., 2020). Recent advances in 3D brain models are (i) combining organoids from different regions of the brain (Kim et al., 2019), recapitulating the connection between different types of neurons or neurons and muscles - assembloids (Andersen et al., 2020; Miura et al., 2020), (ii) modeling of chorion plexus (Pellegrini et al., 2020a) and organoids secreting cerebral spinal fluid (Pellegrini et al., 2020b) (iii) incorporation of immunocompetent cells—microglia (Abreu et al., 2018; Ormel et al., 2018; Bodnar et al., 2021) (iv) vascularization of brain organoids (Cakir et al., 2019; Ham et al., 2020) and models of brain organoids with blood brain barrier (BBB) (Bergmann et al., 2018; Nzou et al., 2020). However, in neurotoxicology, the reproducibility of the system is of highest importance, and therefore the developers are aiming for simplicity as far as possible but complex enough to recapitulate human-relevant cellular processes and functionality. It is clear that simple monolayer cultures have limitations and are far away from representing the human brain in terms of architecture and functionality, but they can still be very useful when combined in a battery of tests. The same applies for brain MPS. The main question to be asked here: how to find the balance between the complexity and simplicity needed to have robust, reproducible systems that can be applied for chemical screening in a higher throughput manner.
FIGURE 1. Schematic representation of 3D brain models as a component of brain microphysiological systems (brain MPS). Different levels of complexity (spheroids, organoids, microfluidics, brain-on-chip) are shown.
Why are New More Advanced Cell Models Needed in (Developmental) Neurotoxicology?
Developmental Neurotoxicity (DNT) is an issue not adequately covered by existing testing strategies. Current DNT testing for risk assessment purposes is entirely animal-based and is not a standard requirement, not because of a lack of interest in the hazard but a lack of adequate testing opportunities (Smirnova et al., 2014). An important restriction for routine DNT assessment of drugs and chemicals are the high costs of the current regulatory test strategy according to the DNT guidelines (OECD TG 426 and US EPA 712-C-98-239) (EPA, 1998; OECD, 2007). But there are also scientific concerns regarding the relevance of these studies for human health effects. As current guidelines often do not provide sufficient information to facilitate regulatory decision-making, new approach methodologies (NAMs) to assess DNT are considered (EPA, 2020; Masjosthusmann et al., 2020). Experts in the field have recommended the use of a battery of in vitro tests covering the cellular key events of neural development to be used for the initial prioritization of high-risk environmental chemicals for DNT testing (Bal-Price et al., 2018a; Bal-Price et al., 2018b). These tests, are mostly based on traditional monolayer cell cultures, have been developed in different laboratories and measure different endpoints of DNT. This means the important step of harmonization and combination of the tests is necessary. Moreover, the important interactions between different cell types and key events during development are often missing. For this reason, fewer more complex unified MPS covering most key neurodevelopmental events would streamline this process of DNT testing. Some of these key events e.g., myelination and synapse formation might be better modeled in 3D. Myelination is a complex process which is hard to achieve in vitro, especially in 2D cultures. Few brain and spinal cord organoids have been developed that show differentiation and maturation of oligodendrocytes with the formation of the myelin sheath (Pamies et al., 2017; Madhavan et al., 2018; Chesnut et al., 2021; James et al., 2021; Shaker et al., 2021). Active synapses can be formed in both 2D and 3D cultures, where synaptogenesis can be assessed with high content imaging (Harrill et al., 2011; Verstraelen et al., 2018) or multi-electrode arrays (Brown et al., 2016). However, synaptogenesis in 3D can be modeled in more physiologically relevant cellular organization (network formation, pattering and layering of different brain regions described in organoids, which better reflects in vivo tissue complexity). One might suggest that there are certain advantages in studying synaptogenesis in 3D, although the analysis becomes more complex (see below in optimization of assays).
Although 3D models have been developed, very few compounds have been tested in these systems and there is currently no well-developed DNT test available using these human models (Bal-Price et al., 2018a). Thus, it is the most pressing to develop harmonized, human-relevant and relatively simple-to-use, transferable MPS for DNT.
Challenges and Opportunities for 3D Brain Models
Even though 3D brain models have great potential to enhance the DNT assessment by more closely mimicking the in vivo situation, there are currently several limitations and challenges to address (Table 1).
TABLE 1. Summary of current challenges and future directions of 3D brain models.
Standardization and Reproducibility
The major challenge to move 3D brain models towards regulatory acceptance for DNT testing is standardization and reproducibility. The more complex the system is the more rigorous quality control steps must be taken to ensure the system’s validity and predictivity of the outcomes. Although the reproducibility of brain organoids has been improving over the last years (Velasco et al., 2019; Yoon et al., 2019), especially through harmonization of the existing protocols, and commercially available kits for differentiation (e.g., StemCell Technology kit for cortical organoids), they are still heterogeneous cultures due to the nature of the system development: diversity of protocols, increased variability because of different donors of iPSC etc. The recent updated guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0) aims to provide guidance in assuring the reproducibility of in vitro systems, including the complex ones such as iPSC-derived models and MPS (Pamies et al., 2022). When it comes to different donors, the reproducibility between the cell lines is the key, as it has been shown that already on iPSC level, there is a high level of donor-to-donor, or even clone-to-clone variability (Volpato and Webber, 2020). The scientists are refining the protocols to improve the physiological relevance and to generate organoids from different parts of the brain (e.g., cerebellar organoids by Qian et al., 2016; Quadrato et al., 2017; Silva et al., 2020). The 3D structure complicates the reproducibility further as most techniques, e.g., gyratory shaking, scaffolding, hanging drop techniques, and spontaneous aggregating, allow cells within the organoids to migrate to its positions and initiate the self-organized cell-cell interactions. Since these models lack the complete chemotaxis present during development in vivo the variability between the organoid cell-architecture can be high. Several groups are using microfluidic chip-platforms to explore how the gradients of different substances such as growth factors and chemokines can control the migration and differentiation of cells (Kilic et al., 2016; Cho et al., 2021). The use of bioprinting methods are other ways to force cells into specific positions and shapes (Han and Hsu, 2017; Khan et al., 2021; Roversi et al., 2021), however, this can lead to artifacts as the cell’s natural potential might be restrained. All revisions of existing protocols for differentiation and cell culturing need to go through new standardization processes as even small changes can introduce new variables.
Cost and Complexity
Another limitation when considering these systems for toxicology are still relatively high costs and complex protocols, especially for organ-on-chip systems. The protocols for human iPSCs-derived brain organoids are naturally long as those systems recapitulate human brain development, and the differentiation and maturation of different lineages in vivo is a long process stretching over the whole embryonic, fetal and first years of postnatal development (Rodier, 1980; Rice and Barone, 2000). Since most protocols currently are using iPSC cells, adequately trained cellular biologists are needed to complete the task. Growth factors and supplements are costly but are required for models which sometimes are kept in culture for up to a year (Lancaster and Knoblich, 2014; Madhavan et al., 2018; Trujillo et al., 2019; Giandomenico et al., 2021). For pharmaceutical and industrial companies, the complex MPS models might be more feasible on contract research organizations (CROs) bases than setting it up in house, as many MPS are still in a developing stage. By outsourcing the research and development activities to a third party, the companies can stay competitive and flexible in terms of novel techniques and profit (Clearwater International, 2021). Most academic labs developing new cell models and assays do not have the required quality management or expertise to provide the expected level of services. Moreover, for an assay to be useful in a regulatory context, the transferability to another laboratory is generally needed as part of the validation process. This can be challenging if the cell model is too complex and/or need specific laboratory equipment. The National Center for Advancing Translational Sciences (NCATS), National Institute of Health (NIH) awarded two Tissue Chip Testing Centers (https://ncats.nih.gov/tissuechip/projects/centers/2018) with the aim to independently reproduce previous published MPS data to assess their robustness, portability of the technology, develop best practices, and provide input for further improvement (Low and Tagle, 2017). Even though these new technologies need performance accreditation, the classical validation process will have to be adjusted to a fit-for-purpose validation (discussed in NAS, 2017; Marx et al., 2020).
Throughput
One of the advantages with the in vitro approach is the increased throughput for testing chemicals. Many of the regulatory programs such as ToxCast (https://www.epa.gov/chemical-research/toxcast-chemicals) and Tox21 (https://tox21.gov/) are using robotic assays that can screen thousands of chemicals in a very short time. However, the cell systems used with these assays are often simple. Due to the complex nature of 3D cultures and MPS the throughput is still a limitation. One way to overcome this, is to develop microfluidic and automatic handling machines adapted for the organoids. Although brain-on-chip and microfluidic systems of neural cultures exist, they are limitedly utilized for 3D brain models (reviewed in Miccoli et al., 2018; Osaki et al., 2018). The use of more automatic culturing techniques will likely increase the reproducibility and standardization as well.
Optimization of Assays
Most of the in vitro assays have been developed for monolayer cultures and therefore need to be adapted for the 3D systems. In general, before applying any assay developed for monolayer cultures, an extensive quality control of the intended application in 3D needs to be established. Two of them will be discussed as examples. Immunohistochemistry is labor intense and technically challenging in the 3D cultures. Some limiting factors include issues with antibody penetration and laser power of a confocal microscope which makes it difficult to image through the entire depth of an organoid. To overcome this, many groups are making cryosections of the organoids (Pamies et al., 2020), however, with the risk to damage the neurites or cell-cell interactions. Another option is tissue clearing processing which is required to obtain high quality images (Lallemant et al., 2020). However, this process is labor intensive, and time consuming. The enhanced solution could be generation of reporter lines, which may allow to follow different cell linages during development and after toxicant treatment. Imaging the reporter lines in combination with tissue clearing can overcome some of the laborious and expensive antibody staining and cryosectioning methods. Such approach is demanding during the development stage but easy to adapt, when ready to use, it can also increase the throughput and enable high content imaging for several processes in parallel such as neuronal differentiation, synaptogenesis, gliosis/gliagenesis, oligodendrogenesis and myelination, neurite formation and outgrowth. However, to improve the reproducibility and standardization, the introduction of reporter genes should be harmonized between 3D models (Fischer et al., 2019).
Multi-electrode array (MEA) is the most common assay used to assess neuronal functionality by measuring the electrical activity in the test system. Traditional MEA plates were designed for monolayer cultures and are broadly used in neuroscience (Halliwell et al., 2021; Taga et al., 2021; Tukker and Westerink, 2021). Furthermore, MEA has been applied to assess neurotoxicity and DNT in high throughput manner (Strickland et al., 2018; Shafer et al., 2019). However, these plates, have major limitations for 3D models. The density of electrodes is low, and the recording occurs only from the area, where the organoid is touching the electrode. It is difficult to get reproducible results, as it is technically hard to plate the organoids exactly the same way from well-to-well and plate-to-plate. One solution is to design an organoid EEG, a multielectrode shell, covering the whole surface of an organoid (Cools et al., 2018). High density multielectrode array might also offer a solution of more robust and reproducible recording in 3D (Sharf et al., 2021). Use of optogenetics to manipulate neural activity within brain organoids is also growing. The more advanced step would be to grow the organoids around the electrodes, so the recording can occur not only from the surface but also from the inside of the organoid. Advances of electrophysiology of brain organoids are further discussed in (Passaro and Stice, 2020).
Translation to in vivo
The translation of in vitro data to in vivo effects has always been a challenge and it is not a MPS specific problem. However, as we foresee that these advanced cell cultures are better predicter of the in vivo processes, the interpretation of the data is crucial. The question is what we should compare the data generated with NAMs to. As the NAMs often are using human cells the species differences to traditional animal models might poses another challenge. In toxicology, the human data is often missing, and we are still extrapolating from the animal despite knowing the animals are poor predictors of the human outcomes (Harrison, 2016; Cavero et al., 2019). For this reason, many pharmaceutical companies have started to request animal based MPS, especially dogs. Animal MPS-derived results can be compared with whole animal responses and would then increase our confidence in the human MPS ability to represent human outcomes. But what if animal MPS perfectly correlates with human MPS response but not with the animal in vivo response or both MPS and in vivo animal models do not correlate with human MPS? This might be the next challenge to address as more data is being generated. Another challenge with iPSC-derived 3D brain models is how the in vitro differentiation process is comparable with human primary cells. Often cells are still immature, when effects are assessed and especially, if neurodegenerative outcomes are investigated, this could be a challenge (reviewed in Doss and Sachinidis, 2019). For example, would aged dopaminergic neurons from human brain behave the same as iPSC-derived ones? The advantage of 3D brain models is that they can be kept in cultures much longer than traditional monolayer cell systems and several groups are reporting on brain organoids differentiated for several months and even a year (Lancaster and Knoblich, 2014; Madhavan et al., 2018; Trujillo et al., 2019). Recently, new methods to senescence cultures further and faster have been explored (Bigagli et al., 2016; Petrini et al., 2017; Burrinha et al., 2019).
Second Generation Microphysiological Systems
Even though the 3D brain models are complex and more in vivo like than the traditional in vitro assays, there is still prospects for enhancement. Optimization of 3D brain organoids toward more complex MPS by combining 3D with microfluidics, chips and introducing missing cellular and barrier components will bring those systems to a true MPS—MPS 2.0 (Table 1 and below).
Immune System
One of the major limitations of most current human in vitro models, not only 3D, is the lack of the immune component. It is crucial for DNT to incorporate immune cells (microglia) into the brain organoids as they play a big role in the developing brain and are key for neuroinflammation a crucial tissue response to environmental stress (Werneburg et al., 2017; Wright-Jin and Gutmann, 2019; Badimon et al., 2020). Protocols for iPSC-derived microglia have been developed (Abud et al., 2017; Haenseler et al., 2017) and publications of microglia incorporation into 3D cultures are emerging (Abreu et al., 2018; Ormel et al., 2018; Bodnar et al., 2021; Xu et al., 2021). However, other cells of the immune system might be important for the developing brain as well (Zhou et al., 2021) and we might see more research going in this direction with incorporating of T and B cells to the MPS.
Barriers
Blood Brain Barrier (BBB) and placental barrier are other essential components to include in neurotoxicity and DNT studies. There are several models of BBB developed: co-cultures of neurons, astrocytes, endothelial cells, including transwell (Lippmann et al., 2014; Faal et al., 2019; Ohshima et al., 2019), spheroid (Cho et al., 2017; Nzou et al., 2018; Nzou et al., 2020) and chip-style (Yeon et al., 2012; Wang YI et al., 2017) systems. There are few in vitro models of the placenta barrier (Haider et al., 2018; Turco et al., 2018; Sheridan et al., 2020) but to our knowledge they were never applied in DNT. The placenta protects the fetus from insult, while fetal BBB is not fully developed yet. Thus, any disturbance or loss of functional placenta integrity could contribute to DNT effects. Future research is needed to investigate how xenobiotics can interfere with the interaction of the placenta and the developing fetus, including the brain.
Cellular Composition
3D brain models have different levels of complexity as compared to the in vivo brain. Spheroids are 3D but lack brain architecture while brain organoids have more defined structures such as layering of the cortex (Figure 1). However, all of them lack the physiological relevant cellular composition, i.e., neuron/glia ratio. Thus, all these models need further adjustments to bring them closer to the in vivo brain. Modification of the medium, addition of growth factors and signaling molecules, might help to modulate the ratio and promote glia differentiation to bring it to more physiological-relevant distribution of different cell types (neurons/glia—1:1) (reviewed in von Bartheld et al., 2016). Through the implementation of microfluidic systems, growth factor gradients and biomaterials such as extracellular matrix, the special-temporal signaling may become possible (reviewed in Roth et al., 2021). This may further stimulate the maturation and bring histoarchitecture to a more in vivo like level (Kilic et al., 2016; Cho et al., 2021).
Patient Derived iPSC Brain Models
Currently, the different protocols to generate and maintain iPSC may induce variability between different cell lines. Therefore, there can be a challenge to understand if a cell line derived from a specific patient is behaving differently due to the disease or the reprograming and maintenance protocols used. Even if the same protocol is used the different clones from the same patient have shown differences due to reasons we still have limited knowledge about (reviewed in Doss and Sachinidis, 2019; Volpato and Webber, 2020). The use of CRISPR/Cas9 modified isogenic cell lines is one possible solution to overcome this issue (Wang P et al., 2017). However for many diseases, the genetic contribution is more complex than a mutation in one gene or even unknown (idiopathic autism as an example). Another option to increase power of the experiments conducted in patient-derived iPSC lines is to increase number of donors and clones used for experimental set-ups. At least six donors per condition should be considered. As the protocols get more standardized and the reproducibility of 3D brain models enhanced, the use of patient-derived iPSC for neurodevelopmental disorders will likely increase.
Combination of 3D Brain Models With Other Advanced Technologies (Single Cell Sequencing, High Throughput High Content Imaging, Bioengineering)
Over the last decade there has been tremendous evolution in cell-based techniques. It is foreseen that these will be further developed and be more commonly applied in different fields including neurotoxicology and DNT. Going forward there will likely be more real time non-invasive measurements in brain models, e.g., tracing of individual cells for an extended period of time using live-imaging techniques. Other high-content techniques such as single cell sequencing and omics methods will get cheaper and simpler to use. Single cell sequencing use in combination with brain organoids is increasing, as it allows to track the cell-specific molecular changes as well as lineage composition etc. (Kanton et al., 2019; Kanton et al., 2020; Sawada et al., 2020). Moreover, the culturing techniques to generate 3D structures in a more physiological relevant architecture will be developed. Novel endpoints more linked to human data and diagnosis are expected to be developed, such as biomarker discovery. Once substantial MPS data has been generated, it has the potential to also enhance the establishment of more in vivo relevant approaches.
Organ-Organ Interactions
It is well recognized that organ-organ interactions can influence the toxicity of chemicals, e.g., the metabolic activity of the liver, filtration by the kidney, and the microbiome in the gut. The combination of different organs, including the brain, as in the human-on-chip approach (Novak et al., 2020), will be more broadly used. For the developing brain, not only the other organs within the fetus are important, the interaction with the mother’s physiology including the hormonal system, inflammatory responses, and stress also play a role. The future MPS will likely incorporate many of these factors known to contribute to DNT.
Conclusion
The 3D brain models recapitulating the human in vivo brain are increasingly applied in the fields of neuroscience, neurotoxicology and neurological diseases. However, there are still several improvements to aim for: increased reproducibility and standardization, reduced costs, increased throughput, and assay optimizations. Certainly, those models will continue to enhance, get more physiological relevant with, e.g., incorporation of immune cells, engineered cells, and broadly used patient-derived iPSC. The translation to the human patients will hopefully support the development of new biomarkers, personalized medicine, mechanistic knowledge about neurological disorders, potential treatments and an understanding how xenobiotic exposure contributes to DNT.
Author Contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Funding
LS was supported by an EPA-STAR grant #R83950501.
Conflict of Interest
HH is named inventor on a patent by Johns Hopkins University on the production of BrainSpheres, which is licensed to AxoSim, New Orleans, LA, United States, and receives royalty shares. HH and LS consult AxoSim.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Reference
Abreu, C. M., Gama, L., Krasemann, S., Chesnut, M., Odwin-Dacosta, S., Hogberg, H. T., et al. (2018). Microglia Increase Inflammatory Responses in iPSC-Derived Human BrainSpheres. Front. Microbiol. 9, 2766. doi:10.3389/fmicb.2018.02766
Abud, E. M., Ramirez, R. N., Martinez, E. S., Healy, L. M., Nguyen, C. H. H., Newman, S. A., et al. (2017). iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases. Neuron 94 (2), 278–293. doi:10.1016/j.neuron.2017.03.042
Andersen, J., Revah, O., Miura, Y., Thom, N., Amin, N. D., Kelley, K. W., et al. (2020). Generation of Functional Human 3D Cortico-Motor Assembloids. Cell 183 (7), 1913–1929. doi:10.1016/j.cell.2020.11.017
Badimon, A., Strasburger, H. J., Ayata, P., Chen, X., Nair, A., Ikegami, A., et al. (2020). Negative Feedback Control of Neuronal Activity by Microglia. Nature 586 (7829), 417–423. doi:10.1038/s41586-020-2777-8
Bal-Price, A., Hogberg, H. T., Crofton, K. M., Daneshian, M., FitzGerald, R. E., Fritsche, E., et al. (2018a). Recommendation on Test Readiness Criteria for New Approach Methods in Toxicology: Exemplified for Developmental Neurotoxicity. ALTEX 35 (3), 306–352. doi:10.14573/altex.1712081
Bal-Price, A., Pistollato, F., Sachana, M., Bopp, S. K., Munn, S., and Worth, A. (2018b). Strategies to Improve the Regulatory Assessment of Developmental Neurotoxicity (DNT) Using In Vitro Methods. Toxicol. Appl. Pharmacol. 354, 7–18. doi:10.1016/j.taap.2018.02.008
Bergmann, S., Lawler, S. E., Qu, Y., Fadzen, C. M., Wolfe, J. M., Regan, M. S., et al. (2018). Blood-brain-barrier Organoids for Investigating the Permeability of CNS Therapeutics. Nat. Protoc. 13 (12), 2827–2843. doi:10.1038/s41596-018-0066-x
Bigagli, E., Luceri, C., Scartabelli, T., Dolara, P., Casamenti, F., Pellegrini-Giampietro, D. E., et al. (2016). Long-term Neuroglial Cocultures as a Brain Aging Model: Hallmarks of Senescence, MicroRNA Expression Profiles, and Comparison with In Vivo Models. Gerona 71 (1), 50–60. doi:10.1093/gerona/glu231
Bodnar, B., Zhang, Y., Liu, J., Lin, Y., Wang, P., Wei, Z., et al. (2021). Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids from Human Induced Pluripotent Stem Cells. Front. Cel. Neurosci. 15, 682272. doi:10.3389/fncel.2021.682272
Brown, J. P., Hall, D., Frank, C. L., Wallace, K., Mundy, W. R., and Shafer, T. J. (2016). Editor's Highlight: Evaluation of a Microelectrode Array-Based Assay for Neural Network Ontogeny Using Training Set Chemicals. Toxicol. Sci. 154 (1), 126–139. doi:10.1093/toxsci/kfw147
Burrinha, T., Gomes, R., Terrasso, A. P., and Almeida, C. G. (2019). Neuronal Aging Potentiates Beta-Amyloid Generation via Amyloid Precursor Protein Endocytosis. bioRxiv, 616540. doi:10.1101/616540
Cakir, B., Xiang, Y., Tanaka, Y., Kural, M. H., Parent, M., Kang, Y.-J., et al. (2019). Engineering of Human Brain Organoids with a Functional Vascular-like System. Nat. Methods 16 (11), 1169–1175. doi:10.1038/s41592-019-0586-5
Cavero, I., Guillon, J.-M., and Holzgrefe, H. H. (2019). Human Organotypic Bioconstructs from Organ-On-Chip Devices for Human-Predictive Biological Insights on Drug Candidates. Expert Opin. Drug Saf. 18 (8), 651–677. doi:10.1080/14740338.2019.1634689
Chesnut, M., Paschoud, H., Repond, C., Smirnova, L., Hartung, T., Zurich, M.-G., et al. (2021). Human IPSC-Derived Model to Study Myelin Disruption. Int. J. Mol. Sci. 22 (17), 9473. doi:10.3390/ijms22179473
Cho, C.-F., Wolfe, J. M., Fadzen, C. M., Calligaris, D., Hornburg, K., Chiocca, E. A., et al. (2017). Blood-brain-barrier Spheroids as an In Vitro Screening Platform for Brain-Penetrating Agents. Nat. Commun. 8, 15623. doi:10.1038/ncomms15623
Cho, A.-N., Jin, Y., An, Y., Kim, J., Choi, Y. S., Lee, J. S., et al. (2021). Microfluidic Device with Brain Extracellular Matrix Promotes Structural and Functional Maturation of Human Brain Organoids. Nat. Commun. 12 (1), 4730. doi:10.1038/s41467-021-24775-5
Clearwater International (2021). A Clearwater International Healthcare Team Report Outsourced Pharma Services. Available at: https://www.clearwaterinternational.com/assets/pdfs/Clearwater-International-Outsourced-Pharma-Services-Report_2021.pdf (Accessed Jan 19, 2022).
Cools, J., Jin, Q., Yoon, E., Alba Burbano, D., Luo, Z., Cuypers, D., et al. (2018). A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells. Adv. Sci. 5 (4), 1700731. doi:10.1002/advs.201700731
Di Lullo, E., and Kriegstein, A. R. (2017). The Use of Brain Organoids to Investigate Neural Development and Disease. Nat. Rev. Neurosci. 18 (10), 573–584. doi:10.1038/nrn.2017.107
Doss, M. X., and Sachinidis, A. (2019). Current Challenges of iPSC-Based Disease Modeling and Therapeutic Implications. Cells 8 (5), 403. doi:10.3390/cells8050403
EPA (1998). Health Effects Guidelines OPPTS 870.6300. Available at: https://www.regulations.gov/document/EPA-HQ-OPPT-2009-0156-0042 (Accessed December 19, 2022).
EPA (2020). Peer Review of the Use of New Approach Methodologies (NAMs) to Derive Extrapolation Factors and Evaluate Developmental Neurotoxicity for Human Health Risk Assessment. Available at: https://www.epa.gov/sap/use-new-approach-methodologies-nams-derive-extrapolation-factors-and-evaluate-developmental (Accessed January 19, 2022).
Faal, T., Phan, D. T. T., Davtyan, H., Scarfone, V. M., Varady, E., Blurton-Jones, M., et al. (2019). Induction of Mesoderm and Neural Crest-Derived Pericytes from Human Pluripotent Stem Cells to Study Blood-Brain Barrier Interactions. Stem Cel Rep. 12 (3), 451–460. doi:10.1016/j.stemcr.2019.01.005
Fischer, J., Heide, M., and Huttner, W. B. (2019). Genetic Modification of Brain Organoids. Front. Cel. Neurosci. 13, 558. doi:10.3389/fncel.2019.00558
Giandomenico, S. L., Sutcliffe, M., and Lancaster, M. A. (2021). Generation and Long-Term Culture of Advanced Cerebral Organoids for Studying Later Stages of Neural Development. Nat. Protoc. 16 (2), 579–602. doi:10.1038/s41596-020-00433-w
Haenseler, W., Sansom, S. N., Buchrieser, J., Newey, S. E., Moore, C. S., Nicholls, F. J., et al. (2017). A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-co-culture-specific Expression Profile and Inflammatory Response. Stem Cel Rep. 8 (6), 1727–1742. doi:10.1016/j.stemcr.2017.05.017
Haider, S., Meinhardt, G., Saleh, L., Kunihs, V., Gamperl, M., Kaindl, U., et al. (2018). Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta. Stem Cel Rep. 11 (2), 537–551. doi:10.1016/j.stemcr.2018.07.004
Halliwell, R. F., Salmanzadeh, H., Coyne, L., and Cao, W. S. (2021). An Electrophysiological and Pharmacological Study of the Properties of Human iPSC-Derived Neurons for Drug Discovery. Cells 10 (8), 1953. doi:10.3390/cells10081953
Ham, O., Jin, Y. B., Kim, J., and Lee, M.-O. (2020). Blood Vessel Formation in Cerebral Organoids Formed from Human Embryonic Stem Cells. Biochem. Biophys. Res. Commun. 521 (1), 84–90. doi:10.1016/j.bbrc.2019.10.079
Hsu, S.-h., and Han, H.-W. (2017). Using 3D Bioprinting to Produce Mini-Brain. Neural Regen. Res. 12 (10), 1595–1596. doi:10.4103/1673-5374.217325
Harrill, J. A., Robinette, B. L., and Mundy, W. R. (2011). Use of High Content Image Analysis to Detect Chemical-Induced Changes in Synaptogenesis In Vitro. Toxicol. Vitro 25 (1), 368–387. doi:10.1016/j.tiv.2010.10.011
Harrison, R. K. (2016). Phase II and Phase III Failures: 2013-2015. Nat. Rev. Drug Discov. 15 (12), 817–818. doi:10.1038/nrd.2016.184
James, O. G., Selvaraj, B. T., Magnani, D., Burr, K., Connick, P., Barton, S. K., et al. (2021). iPSC-derived Myelinoids to Study Myelin Biology of Humans. Develop. Cel 56 (9), 1346–1358. doi:10.1016/j.devcel.2021.04.006
Kanton, S., Boyle, M. J., He, Z., Santel, M., Weigert, A., Sanchís-Calleja, F., et al. (2019). Organoid Single-Cell Genomic Atlas Uncovers Human-specific Features of Brain Development. Nature 574 (7778), 418–422. doi:10.1038/s41586-019-1654-9
Kanton, S., Treutlein, B., and Camp, J. G. (2020). Single-cell Genomic Analysis of Human Cerebral Organoids. Methods Cel Biol. 159, 229–256. doi:10.1016/bs.mcb.2020.03.013
Khan, I., Prabhakar, A., Delepine, C., Tsang, H., Pham, V., and Sur, M. (2021). A Low-Cost 3D Printed Microfluidic Bioreactor and Imaging Chamber for Live-Organoid Imaging. Biomicrofluidics 15 (2), 024105. doi:10.1063/5.0041027
Kilic, O., Pamies, D., Lavell, E., Schiapparelli, P., Feng, Y., Hartung, T., et al. (2016). Brain-on-a-chip Model Enables Analysis of Human Neuronal Differentiation and Chemotaxis. Lab. Chip 16 (21), 4152–4162. doi:10.1039/c6lc00946h
Kim, H., Xu, R., Padmashri, R., Dunaevsky, A., Liu, Y., Dreyfus, C. F., et al. (2019). Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins. Stem Cel Rep. 12 (5), 890–905. doi:10.1016/j.stemcr.2019.04.011
Koo, B., Choi, B., Park, H., and Yoon, K. J. (2019). Past, Present, and Future of Brain Organoid Technology. Mol. Cell 42 (9), 617–627. doi:10.14348/molcells.2019.0162
Lallemant, L., Lebreton, C., and Garfa-Traoré, M. (2020). Comparison of Different Clearing and Acquisition Methods for 3D Imaging of Murine Intestinal Organoids. J. Biol. Methods 7 (4), e141. doi:10.14440/jbm.2020.334
Lancaster, M. A., and Knoblich, J. A. (2014). Generation of Cerebral Organoids from Human Pluripotent Stem Cells. Nat. Protoc. 9 (10), 2329–2340. doi:10.1038/nprot.2014.158
Lippmann, E. S., Al-Ahmad, A., Azarin, S. M., Palecek, S. P., and Shusta, E. V. (2014). A Retinoic Acid-Enhanced, Multicellular Human Blood-Brain Barrier Model Derived from Stem Cell Sources. Sci. Rep. 4, 4160. doi:10.1038/srep04160
Low, L. A., and Tagle, D. A. (2017). Organs-on-chips: Progress, Challenges, and Future Directions. Exp. Biol. Med. (Maywood) 242 (16), 1573–1578. doi:10.1177/1535370217700523
Madhavan, M., Nevin, Z. S., Shick, H. E., Garrison, E., Clarkson-Paredes, C., Karl, M., et al. (2018). Induction of Myelinating Oligodendrocytes in Human Cortical Spheroids. Nat. Methods 15 (9), 700–706. doi:10.1038/s41592-018-0081-4
Marx, U., Akabane, T., Andersson, T. B., Baker, E., Beilmann, M., Beken, S., et al. (2020). Biology-inspired Microphysiological Systems to advance Medicines for Patient Benefit and Animal Welfare. ALTEX 37 (3), 365–394. doi:10.14573/altex.2001241
Marx, V. (2020). Reality Check for Organoids in Neuroscience. Nat. Methods 17 (10), 961–964. doi:10.1038/s41592-020-0964-z
Masjosthusmann, S., Blum, J., Bartmann, K., Dolde, X., Holzer, A. K., Stürzl, L. C., et al. (2020). Establishment of an A Priori Protocol for the Implementation and Interpretation of an In‐vitro Testing Battery for the Assessment of Developmental Neurotoxicity. EFSA Support. Publ. 17 (10), 1938E. doi:10.2903/sp.efsa.2020.EN-1938
Miccoli, B., Braeken, D., and Li, Y.-C. E. (2019). Brain-on-a-chip Devices for Drug Screening and Disease Modeling Applications. CPD 24 (45), 5419–5436. doi:10.2174/1381612825666190220161254
Miura, Y., Li, M.-Y., Birey, F., Ikeda, K., Revah, O., Thete, M. V., et al. (2020). Generation of Human Striatal Organoids and Cortico-Striatal Assembloids from Human Pluripotent Stem Cells. Nat. Biotechnol. 38 (12), 1421–1430. doi:10.1038/s41587-020-00763-w
NAS (2017). Using 21st Century Science to Improve Risk-Related Evaluations. Washington, DC, USA: National Academies Press.
Novak, R., Ingram, M., Marquez, S., Das, D., Delahanty, A., Herland, A., et al. (2020). Robotic Fluidic Coupling and Interrogation of Multiple Vascularized Organ Chips. Nat. Biomed. Eng. 4 (4), 407–420. doi:10.1038/s41551-019-0497-x
Nzou, G., Wicks, R. T., Wicks, E. E., Seale, S. A., Sane, C. H., Chen, A., et al. (2018). Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling. Sci. Rep. 8 (1), 7413. doi:10.1038/s41598-018-25603-5
Nzou, G., Wicks, R. T., VanOstrand, N. R., Mekky, G. A., Seale, S. A., El-Taibany, A., et al. (2020). Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction. Sci. Rep. 10 (1), 9766. doi:10.1038/s41598-020-66487-8
OECD (2007). Test No. 426: Developmental Neurotoxicity Study. Available at: https://www.oecd-ilibrary.org/environment/test-no-426-developmental-neurotoxicity-study_9789264067394-en (Accessed January 19, 2022).
Ohshima, M., Kamei, S., Fushimi, H., Mima, S., Yamada, T., and Yamamoto, T. (2019). Prediction of Drug Permeability Using In Vitro Blood-Brain Barrier Models with Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells. BioRes. Open Access 8 (1), 200–209. doi:10.1089/biores.2019.0026
Ormel, P. R., Vieira de Sá, R., van Bodegraven, E. J., Karst, H., Harschnitz, O., Sneeboer, M. A. M., et al. (2018). Microglia Innately Develop within Cerebral Organoids. Nat. Commun. 9 (1), 4167. doi:10.1038/s41467-018-06684-2
Osaki, T., Shin, Y., Sivathanu, V., Campisi, M., and Kamm, R. D. (2018). In Vitro Microfluidic Models for Neurodegenerative Disorders. Adv. Healthc. Mater. 7 (2), 1700489. doi:10.1002/adhm.201700489
Pamies, D., Barreras, P., Block, K., Makri, G., Kumar, A., Wiersma, D., et al. (2017). A Human Brain Microphysiological System Derived from Induced Pluripotent Stem Cells to Study Neurological Diseases and Toxicity. ALTEX 34 (3), 362–376. doi:10.14573/altex.1609122
Pamies, D., Zurich, M.-G., and Hartung, T. (2020). Organotypic Models to Study Human Glioblastoma: Studying the Beast in its Ecosystem. iScience 23 (10), 101633. doi:10.1016/j.isci.2020.101633
Pamies, D., Leist, M., Coecke, S., Bowe, G., Allen, D. G., Gstraunthaler, G., et al. (2022). Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0). Altex 39 (1), 30–70. doi:10.14573/altex.2111011
Paşca, A. M., Sloan, S. A., Clarke, L. E., Tian, Y., Makinson, C. D., Huber, N., et al. (2015). Functional Cortical Neurons and Astrocytes from Human Pluripotent Stem Cells in 3D Culture. Nat. Methods 12 (7), 671–678. doi:10.1038/nmeth.3415
Passaro, A. P., and Stice, S. L. (2020). Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements. Front. Neurosci. 14, 622137. doi:10.3389/fnins.2020.622137
Pellegrini, L., Albecka, A., Mallery, D. L., Kellner, M. J., Paul, D., Carter, A. P., et al. (2020a). SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27 (6), 951–961. doi:10.1016/j.stem.2020.10.001
Pellegrini, L., Bonfio, C., Chadwick, J., Begum, F., Skehel, M., and Lancaster, M. A. (2020b). Human CNS Barrier-Forming Organoids with Cerebrospinal Fluid Production. Science 369 (6500), eaaz5626. doi:10.1126/science.aaz5626
Petrini, S., Borghi, R., D’Oria, V., Restaldi, F., Moreno, S., Novelli, A., et al. (2017). Aged Induced Pluripotent Stem Cell (iPSCs) as a New Cellular Model for Studying Premature Aging. Aging 9 (5), 1453–1469. doi:10.18632/aging.101248
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., et al. (2016). Brain-Region-Specific Organoids Using Mini-Bioreactors for Modeling ZIKV Exposure. Cell 165 (5), 1238–1254. doi:10.1016/j.cell.2016.04.032
Quadrato, G., Nguyen, T., Macosko, E. Z., Sherwood, J. L., Min Yang, S., Berger, D. R., et al. (2017). Cell Diversity and Network Dynamics in Photosensitive Human Brain Organoids. Nature 545 (7652), 48–53. doi:10.1038/nature22047
Rice, D., and Barone, S. (2000). Critical Periods of Vulnerability for the Developing Nervous System: Evidence from Humans and Animal Models. Environ. Health Perspect. 108 (Suppl. 3), 511–533. doi:10.1289/ehp.00108s3511
Rodier, P. M. (1980). Chronology of Neuron Development: Animal Studies and Their Clinical Implications. Dev. Med. Child. Neurol. 22 (4), 525–545. doi:10.1111/j.1469-8749.1980.tb04363.x
Roth, J. G., Huang, M. S., Li, T. L., Feig, V. R., Jiang, Y., Cui, B., et al. (2021). Advancing Models of Neural Development with Biomaterials. Nat. Rev. Neurosci. 22 (10), 593–615. doi:10.1038/s41583-021-00496-y
Roversi, K., Ebrahimi Orimi, H., Falchetti, M., Lummertz da Rocha, E., Talbot, S., and Boutopoulos, C. (2021). Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST). Micromachines 12 (8), 865. doi:10.3390/mi12080865
Sawada, T., Chater, T. E., Sasagawa, Y., Yoshimura, M., Fujimori-Tonou, N., Tanaka, K., et al. (2020). Developmental Excitation-Inhibition Imbalance Underlying Psychoses Revealed by Single-Cell Analyses of Discordant Twins-Derived Cerebral Organoids. Mol. Psychiatry 25 (11), 2695–2711. doi:10.1038/s41380-020-0844-z
Shafer, T. J., Brown, J. P., Lynch, B., Davila-Montero, S., Wallace, K., and Friedman, K. P. (2019). Evaluation of Chemical Effects on Network Formation in Cortical Neurons Grown on Microelectrode Arrays. Toxicol. Sci. 169 (2), 436–455. doi:10.1093/toxsci/kfz052
Shaker, M. R., Pietrogrande, G., Martin, S., Lee, J.-H., Sun, W., and Wolvetang, E. J. (2021). Rapid and Efficient Generation of Myelinating Human Oligodendrocytes in Organoids. Front. Cel. Neurosci. 15, 631548. doi:10.3389/fncel.2021.631548
Sharf, T., van der Molen, T., Glasauer, S. M. K., Guzman, E., Buccino, A. P., Luna, G., et al. (2021). Human Brain Organoid Networks. bioRxiv, 428643. doi:10.1101/2021.01.28.428643
Sheridan, M. A., Fernando, R. C., Gardner, L., Hollinshead, M. S., Burton, G. J., Moffett, A., et al. (2020). Establishment and Differentiation of Long-Term Trophoblast Organoid Cultures from the Human Placenta. Nat. Protoc. 15 (10), 3441–3463. doi:10.1038/s41596-020-0381-x
Shou, Y., Liang, F., Xu, S., and Li, X. (2020). The Application of Brain Organoids: From Neuronal Development to Neurological Diseases. Front. Cel Dev. Biol. 8 (1092), 579659. doi:10.3389/fcell.2020.579659
Silva, T. P., Fernandes, T. G., Nogueira, D. E. S., Rodrigues, C. A. V., Bekman, E. P., Hashimura, Y., et al. (2020). Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining. JoVE 160. doi:10.3791/61143
Smirnova, L., Hogberg, H. T., Leist, M., and Hartung, T. (2014). Developmental Neurotoxicity - Challenges in the 21st century and In Vitro Opportunities. ALTEX 31 (2), 129–156. doi:10.14573/altex.1403271
Strickland, J. D., Martin, M. T., Richard, A. M., Houck, K. A., and Shafer, T. J. (2018). Screening the ToxCast Phase II Libraries for Alterations in Network Function Using Cortical Neurons Grown on Multi-Well Microelectrode Array (mwMEA) Plates. Arch. Toxicol. 92 (1), 487–500. doi:10.1007/s00204-017-2035-5
Sun, N., Meng, X., Liu, Y., Song, D., Jiang, C., and Cai, J. (2021). Applications of Brain Organoids in Neurodevelopment and Neurological Diseases. J. Biomed. Sci. 28 (1), 30. doi:10.1186/s12929-021-00728-4
Taga, A., Habela, C. W., Johns, A., Liu, S., O'Brien, M., and Maragakis, N. J. (2021). Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons. JoVE 174. doi:10.3791/62726
Trujillo, C. A., Gao, R., Negraes, P. D., Gu, J., Buchanan, J., Preissl, S., et al. (2019). Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development. Cell Stem Cell 25 (4), 558–569. doi:10.1016/j.stem.2019.08.002
Tukker, A. M., and Westerink, R. H. S. (2021). Novel Test Strategies for In Vitro Seizure Liability Assessment. Expert Opin. Drug Metab. Toxicol. 17 (8), 923–936. doi:10.1080/17425255.2021.1876026
Turco, M. Y., Gardner, L., Kay, R. G., Hamilton, R. S., Prater, M., Hollinshead, M. S., et al. (2018). Trophoblast Organoids as a Model for Maternal-Fetal Interactions during Human Placentation. Nature 564 (7735), 263–267. doi:10.1038/s41586-018-0753-3
Velasco, S., Kedaigle, A. J., Simmons, S. K., Nash, A., Rocha, M., Quadrato, G., et al. (2019). Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. Nature 570 (7762), 523–527. doi:10.1038/s41586-019-1289-x
Verstraelen, P., Van Dyck, M., Verschuuren, M., Kashikar, N. D., Nuydens, R., Timmermans, J.-P., et al. (2018). Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture. Front. Neurosci. 12, 389. doi:10.3389/fnins.2018.00389
Volpato, V., and Webber, C. (2020). Addressing Variability in iPSC-Derived Models of Human Disease: Guidelines to Promote Reproducibility. Dis. Model. Mech. 13 (1), dmm042317. doi:10.1242/dmm.042317
von Bartheld, C. S., Bahney, J., and Herculano-Houzel, S. (2016). The Search for True Numbers of Neurons and Glial Cells in the Human Brain: A Review of 150 Years of Cell Counting. J. Comp. Neurol. 524 (18), 3865–3895. doi:10.1002/cne.24040
Wang P, P., Mokhtari, R., Pedrosa, E., Kirschenbaum, M., Bayrak, C., Zheng, D., et al. (2017). CRISPR/Cas9-mediated Heterozygous Knockout of the Autism Gene CHD8 and Characterization of its Transcriptional Networks in Cerebral Organoids Derived from iPS Cells. Mol. Autism 8, 11. doi:10.1186/s13229-017-0124-1
Wang YI, Y. I., Abaci, H. E., and Shuler, M. L. (2017). Microfluidic Blood-Brain Barrier Model Provides In Vivo‐like Barrier Properties for Drug Permeability Screening. Biotechnol. Bioeng. 114 (1), 184–194. doi:10.1002/bit.26045
Werneburg, S., Feinberg, P. A., Johnson, K. M., and Schafer, D. P. (2017). A Microglia-Cytokine axis to Modulate Synaptic Connectivity and Function. Curr. Opin. Neurobiol. 47, 138–145. doi:10.1016/j.conb.2017.10.002
Wright-Jin, E. C., and Gutmann, D. H. (2019). Microglia as Dynamic Cellular Mediators of Brain Function. Trends Mol. Med. 25 (11), 967–979. doi:10.1016/j.molmed.2019.08.013
Xu, R., Boreland, A. J., Li, X., Erickson, C., Jin, M., Atkins, C., et al. (2021). Developing Human Pluripotent Stem Cell-Based Cerebral Organoids with a Controllable Microglia Ratio for Modeling Brain Development and Pathology. Stem Cel Rep. 16 (8), 1923–1937. doi:10.1016/j.stemcr.2021.06.011
Yeon, J. H., Na, D., Choi, K., Ryu, S.-W., Choi, C., and Park, J.-K. (2012). Reliable Permeability Assay System in a Microfluidic Device Mimicking Cerebral Vasculatures. Biomed. Microdevices 14 (6), 1141–1148. doi:10.1007/s10544-012-9680-5
Yoon, S.-J., Elahi, L. S., Pașca, A. M., Marton, R. M., Gordon, A., Revah, O., et al. (2019). Reliability of Human Cortical Organoid Generation. Nat. Methods 16 (1), 75–78. doi:10.1038/s41592-018-0255-0
Keywords: 3D brain model, DNT, neurotoxicity, brain organoids, brain MPS
Citation: Hogberg HT and Smirnova L (2022) The Future of 3D Brain Cultures in Developmental Neurotoxicity Testing. Front. Toxicology 4:808620. doi: 10.3389/ftox.2022.808620
Received: 03 November 2021; Accepted: 12 January 2022;
Published: 27 January 2022.
Edited by:
Timothy J. Shafer, United States Environmental Protection Agency (EPA), United StatesReviewed by:
Joshua Harrill, United States Environmental Protection Agency (EPA), United StatesCopyright © 2022 Hogberg and Smirnova. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Helena T. Hogberg, aGhvZ2JlcjJAamh1LmVkdQ==