Jiaxin Chen1
Dehai Liang- 1Beijing National Laboratory for Molecular Sciences, and the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China
- 2Beijing Key Laboratory of Mineral Environmental Function, and the Key Laboratory of Orogenic Belts and Crustal Evolution, School of Earth and Space Science, Peking University, Beijing, China
The first life was believed to emerge in the early Earth via a process involving synthesis of organic compounds and formation of protocells. However, it is still a puzzle how the protocell with hierarchal structure and desirable functions was spontaneously generated in the non-living environment composed of mainly water and minerals. In this work, using muscovite as an example of minerals, we systemically studied the coacervation of poly (l-lysine) (PLL), quaternized dextran (Q-dextran), and single-stranded oligonucleotide (ss-oligo) on muscovite surface at varying mixing orders. Only when Q-dextran firstly interacts with muscovite surface to form a coating layer, followed by the addition of ss-oligo and PLL, the formed coacervates exhibit distinct and versatile morphologies, including spherical PLL/ss-oligo droplets on the surface, floating PLL/ss-oligo droplets above the Q-dextran/ss-oligo blanket, and PLL/ss-oligo islands surrounded by the Q-dextran/ss-oligo sea. The kinetic pathways to the resulting morphologies are specific in each case. There results suggest that polysaccharide was probably the first biopolymer accumulated on the mineral surface in early Earth. The sugar coating provided a “nest” for protein/peptide and DNA/RNA to from sub-compartments and to further develop advanced functions.
Introduction
Coacervates are usually formed by oppositely charged polyelectrolytes via liquid-liquid phase separations (LLPS) (Sing and Perry, 2020; Rumyantsev et al., 2021). It has been demonstrated that most of the charged biopolymers, such as DNA/RNA, peptides, proteins, lipids, and polysaccharides, are able to form coacervates of varying morphologies and properties (Kruif et al., 2005; Turgeon et al., 2007; Koga et al., 2011; Wang and Wang, 2014; Croguennec et al., 2017; Zhao and Wang, 2017). The formed coacervates are able to enrich molecules, catalyze reactions, and exchange materials with the external environment (Koga et al., 2011; Williams et al., 2012; Black et al., 2014; Tang et al., 2014; Martin et al., 2016; Nakashima et al., 2019; Poudyal et al., 2019). More importantly, the coacervates share the same formation mechanism as the P-granules, Cajal bodies, and other membraneless organelles found in living cells (Brangwynne et al., 2009; Brangwynne, 2013; Hyman et al., 2014; Feric et al., 2016; Saha et al., 2016; Jiang et al., 2017; Nakagawa et al., 2018; Gomes and Shorter, 2019; Sawyer et al., 2019). Therefore, coacervates have been widely applied as membraneless protocell models to explore the origin of life in early Earth (Douliez et al., 2017; Kumar et al., 2018; Alberti et al., 2019; Abbas et al., 2021; Ghosh et al., 2021; van Haren et al., 2021; Yewdall et al., 2021). With a proper combination of the internal components and the external stimuli, coacervate protocells have achieved many living features, like division, evolution, and metabolism (Dzieciol and Mann, 2012; Zwicker et al., 2016; Nakashima et al., 2018; Spoelstra et al., 2019; Chen et al., 2020).
It is generally accepted that the life was emerged from the non-living environment in the prebiotic Earth (Kee and Monnard, 2016; Sahai et al., 2016). The non-living environment in early Earth mainly consists of water, rocks (minerals), and air (Dalai et al., 2016). Life was supposed to originate in aqueous environment, in which process minerals played key roles (Bernal, 1951; Wächtershäuser, 1992; Pontes-Buarque et al., 2000; Franchi and Gallori, 2005; Gomez et al., 2007; Wächtershäuser, 2007; Xu et al., 2013; Sand and Jelavić, 2018; Ertem, 2021). Growing evidences have demonstrated that the mineral surface is able to concentrate organic molecules, catalyze a variety of reactions, and even serve as reactant (Lahav and Chang, 1976; Ertem and Ferris, 1996; Ferris et al., 1996; Hazen et al., 2001; Ferris, 2002; Zhu et al., 2002; Huang and Ferris, 2003; Miyakawa and Ferris, 2003; Hanczyc et al., 2007; Li et al., 2009; Kawamura et al., 2011; Martra et al., 2014; Liao et al., 2016; Dalai and Sahai, 2019). However, it is still a puzzle how the biopolymers assemble into protocell with hierarchal structure and desirable functions in an aqueous environment in the presence of minerals. Most of the biopolymers are soluble in water. Even though the mixing of the oppositely charged polymers at elevated concentrations is able to generate coacervate droplets via LLPS, it is difficult to modulate the structure and properties of the coacervate as LLPS is a spontaneous process. Accumulation of enough biopolymers without hydrolysis is also a limiting step.
The mineral surfaces offer rich opportunities for concentration of biopolymers and possibly their organization into protocells of desirable structures. More than 400 minerals are reported to exist before the origin of life in early Earth (Hazen, 2013). The specific chemical component and the lattice structure render each mineral surface unique physicochemical properties, such as charge, hydrophobicity, functional groups, and dynamic response to environmental conditions like pH, temperature, and ionic strength (Sowerby et al., 2001; Sverjensky, 2005; Sverjensky and Fukushi, 2006; Jonsson et al., 2009). Therefore, a chosen mineral surface should be able selectively absorb the biopolymers via attractive interactions, and trigger the formation of protocells if the combination of biopolymers is appropriate and they are mixed in correct order (Hazen et al., 2001; Zaia, 2004; Gan et al., 2009). More importantly, the relationship between the property of the protocell and the mixing order of biopolymers should shed light on the procedure of life’s emergence in chosen non-living environment. To test this hypothesis, we chose muscovite (KAl2(Si3Al)O10(OH)2) as an example of minerals, and systematically studied the phase separation of poly (l-lysine) (PLL), quaternized dextran (Q-dextran), and single-stranded oligonucleotide (ss-oligo) on muscovite surface at varying mixing orders.
Muscovite is the most abundant rock-forming mineral on Earth and exists before the origin of life (Papineau, 2010; Hazen, 2013). It was proposed that life might originate in the space between mica flakes, especially muscovite mica (Hansma, 2009, 2010, 2013, 2014). Furthermore, muscovite mica is often used as a model mineral to study molecular adsorption, or a sample substrate for atomic force microscopy, surface force apparatus, and X-ray photoelectron spectroscopy (Binnig et al., 1986; Israelachvili and McGuiggan, 1988; Dufrêne, 2002; Wilson et al., 2002). It provides uniform and atomically flat surface when cleaved along the basal plane (De Poel et al., 2014). Such surface is a prerequisite for comparison of the coacervates under varying conditions. PLL, Q-dextran, and ss-oligo are examples of peptide, polysaccharides, and DNA, respectively. Results show that the coacervates with the most distinct morphologies and different dynamics are formed when Q-dextran firstly interacts with muscovite surface, indicating that polysaccharide was probably the first biopolymer synthesized and accumulated on the mineral surface in early Earth.
Materials and methods
Materials
21-mer single-stranded oligonucleotides of random sequence with and without Cy5 labeled on 5′-end were purchased from Invitrogen Biotechnology Co., Ltd. (Shanghai, China). Poly-l-lysine (PLL, MW = 30–70 kDa) with and without fluorescein isothiocyanate (FITC) labeling were purchased from Sigma-Aldrich (St. Louis, MI). Dextran (MW = 10 kDa) and tetramethylrhodamine labeled dextran (TRITC-dextran, MW = 10 kDa) were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China) and Sigma-Aldrich (St. Louis, MO), respectively. Quaternized dextran was synthesized in the lab by following a procedure described elsewhere (Mason et al., 2017). In brief, dextran was dissolved in NaOH solution, followed by the addition of 3-chloro-2-hydroxypropyltrimethylammonium chloride solution at 35°C. The mixture was left to react overnight. The 1H NMR measurement on the final product revealed that each glucose carried 0.7 charge by average. Stock solutions of all samples were prepared in 20 mM MES buffer at pH 6.0 and diluted to known concentrations before use. Muscovite mica was from Hebei, China.
Coacervation on muscovite surface
A lab-built sample cell was used to study the coacervation of PLL, ss-oligo, and Q-dextran. The freshly cleaved muscovite was glued between a cover glass (0.17 mm in thickness) and a glass chip of 5.0 mm in thickness. The glass chip was punctured with circular holes of 6.0 mm in diameter beforehand. The cover glass was the support of muscovite and also facilitated the observation by microscope.
The concentrations of PLL and Q-dextran were fixed at 1.0 mg/ml and 1.8 mg/ml, respectively. The concentrations of ss-oligo were 1.5, 3.0, and 6.0 mg/ml, corresponding to the +/− charge ratios (the number of amine groups over the number of phosphate groups, also named as N/P ratio) of 0.5, 1.0, and 2.0, after forming coacervates with PLL and Q-dextran at equal volume. The charges from the muscovite surface were not counted on the calculation of +/− ratios. Three different routes were applied to vary the mixing orders of PLL, ss-oligo, and ss-oligo.
Route 1: 20 μl Q-dextran was firstly applied on the muscovite surface. 5.0 min later, ss-oligo of same volume was added to formed complex or coacervate with Q-dextran. After incubated for 15 min, 20 μl PLL was added to initiate the formation of biphasic droplets on muscovite surface. The moment of adding PLL was set as time zero. This route was denoted as Q-dextran ← ss-oligo ← PLL.
Route 2: The mixing procedure and the incubation time in each step was the same as those in Route 1, except that the order of adding PLL and Q-dextran was reversed. Therefore, this route was denoted as PLL ← ss-oligo ← Q-dextran. The moment of adding Q-dextran in the last step was set as time zero.
Route 3: Equal volumes of PLL and Q-dextran were premixed in a centrifuge tube. 40 μl mixed solution was then applied to the muscovite surface. After 5 min, 20 μl ss-oligo was added to initiate the formation of biphasic droplet, and the moment of addition was set as time zero. This route was denoted as PLL/Q-dextran ← ss-oligo.
Confocal microscopy
The phase separation of Q-dextran, ss-oligo, and PLL on muscovite surface was monitored by laser scanning confocal microscope (LSCM, Nikon A1R-si). To enable observation by fluorescence, 1.0 wt% FITC-PLL, 1.0 wt% TRITC-Q-dextran, and 1.0 wt% Cy5-ss-oligo were premixed with the corresponding solutions beforehand. The excitation wavelengths for FITC, TRITC, and Cy5 were 490 nm, 557 nm, and 649 nm, respectively. All the settings on LSCM were kept constant during the experiments. The visualization and analysis of the fluorescence images were conducted by using ImageJ software. The average fluorescence intensity at selected time points was calculated to quantitatively describe the growth or dissociation rate of the phases. The fluorescence intensity was normalized by the highest value to compare the behaviors of different phases in one plot. The area occupied by the phases normalized by the total area was also calculated in certain cases.
Results and discussion
There are four different types of polyelectrolytes in the system if treating muscovite surface as a 2D and rigid polyanion. The charge density of PLL (4.8 mmol/g, as calculated by the number of charges per molecular weight of monomer) is almost doubled that of Q-dextran (2.7 mmol/g), and its backbone is also more hydrophobic. Therefore, PLL has the priority to form complex with ss-oligo or attach on the muscovite surface under the same conditions. Similarly, the muscovite surface contains 2.1×1014 charges per square cm (Güven, 1971), far less than that of ss-oligo. It is inferior to ss-oligo in forming complex with polycations. The total negative charges on the muscovite bottom area of 6.0 mm in diameter is 5.9×1013, which is several orders lower than that provided by 20 μl ss-oligo at 1.5 mg/ml. Therefore, the muscovite surface generates negligible effect on the +/− charge ratio. To enhance the effect of muscovite surface on the phase separation process, and also to better mimic the prebiotic condition under which the biopolymers may interact with the mineral surface in sequence, we designed three Routes (Q-dextran ← ss-oligo ← PLL, PLL ← ss-oligo ← Q-dextran, and PLL/Q-dextran ← ss-oligo), allowing Q-dextran, PLL, and their mixture to firstly contact with the muscovite surface, separately, for 5 min, followed by the addition of ss-oligo. In each route, all the conditions are kept constant except that the ss-oligo concentration is varied, to tune the +/− charge ratio from 0.5 to 2.0.
Route 1: Q-dextran ← ss-oligo ← PLL
In this route, 1.8 mg/ml Q-dextran was firstly applied to the muscovite surface for 5 min. Since the diffusion coefficient and the charge density of Q-dextran with Mw = 10 kDa are known (Venturoli and Rippe, 2005; Goins et al., 2008), calculation shows that about half of the negative charges on muscovite surface are neutralized. Ss-oligo at different concentrations is then added to form complex with Q-dextran in the solution and on the surface. Images are taken after incubation for 15 min. With decreasing ss-oligo concentration from 6.0 to 1.5 mg/ml, the coacervation of Q-dextran and ss-oligo on the muscovite surface becomes stronger as demonstrated by the fluorescence intensity and the size of the coacervates (Figure 1). It is reasonable as the +/− charge ratio of the Q-dextran/ss-oligo coacervates increases from 0.25 to unity. The neutralization of the charges results in the formation of coacervates with larger size and higher chain density (Luo and Wang, 2014).
FIGURE 1. Coacervation of Q-dextran and ss-oligo on muscovite surface. The concentrations of ss-oligo were (A) 6.0 mg/ml, (B) 3.0 mg/ml, and (C) 1.5 mg/ml. Q-dextran at 1.8 mg/ml was applied 5.0 min earlier than ss-oligo. The orange and red color denote the traces of TRITC-Q-dextran and Cy5-ss-oligo, respectively. The contract of the right corner in (B) is enhanced to show the distribution of ss-oligo. Scale bars, 20 μm.
1.0 mg/ml PLL was applied to initiate the formation of biphasic droplets. The process is highly dependent on the ss-oligo concentration or the overall +/− charge ratio. In the case of 6.0 mg/mL ss-oligo, the +/− ratio increases from 0.25 to 0.50 after the addition of PLL. The interaction of PLL with the extra ss-oligo molecules results in a heavy coacervation of both PLL/ss-oligo phase and Q-dextran/ss-oligo phase, as demonstrated by the fast growth of the two phases (Figure 2A, 2B, and Movie S1, S2). Note that each phase contains all the three components. For simplicity, only the two major components are mentioned to distinguish the phases. Fluorescence intensity indicates that concentration of ss-oligo is much higher in PLL-rich phase (Figure 2A). Because Q-dextran is coated on the muscovite surface beforehand, the Q-dextran/ss-oligo phase is continuous, while the PLL/ss-oligo phase is isolated. Another interesting feature is that the continuous Q-dextran/ss-oligo phase is not uniform in component distribution, but forms wrinkles around the PLL/ss-oligo phases especially at the later stage (Movie S2). We attribute it to the elevated ss-oligo concentration in PLL/ss-oligo phase, which generates a strong coacervation of Q-dextran and ss-oligo in the periphery of the PLL/ss-oligo phase.
FIGURE 2. Coacervation on muscovite surface via Q-dextran ← ss-oligo ← PLL. The concentrations of ss-oligo, Q-dextran and PLL are 6.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 0.5. (A) Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green and orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red), scale bars: 20 μm. (B) Time dependence of the normalized intensity of PLL and Q-dextran.
In the case of 3.0 mg/ml ss-oligo, the addition of PLL increases the +/− ratio from 0.50 to 1.0, indicating that the excess ss-oligo molecules form complex with PLL at unified +/− ratio. Spherical PLL/ss-oligo coacervates are formed and float in the solution instead attaching on the surface (Movie S3). Both the size and the number of such coacervates increase with time. The spherical shape is maintained throughout the studied time period (Figure 3A). The Q-dextran/ss-oligo phase exhibits quite different behavior. A fast growth and spreading on the muscovite surface occur with time (Figure 3A, 3B and Movie S4). Some spherical droplets are also shown in Q-dextran channel, especially in the later stage, but these are the same droplets observed in PLL channel. It is normal to form spherical droplets containing both PLL and Q-dextran at elevated concentrations at +/- = 1.0. Again, ss-oligo molecules mainly stay with PLL (Figure 3A).
FIGURE 3. Coacervation on muscovite surface via Q-dextran ← ss-oligo ← PLL. The concentrations of ss-oligo, Q-dextran and PLL are 3.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 1.0. (A) Time series of images showing the phase separation by overlay of FITC-PLL/TRIRC-Q-dextran (green and orange), TRIRC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm. (B) Time dependence of the normalized intensity of PLL and Q-dextran.
In the case of 1.5 mg/ml ss-oligo, all ss-oligo molecules form coacervates with Q-dextran. The addition of PLL increases the +/− ratio from 1.0 to 2.0, and polycations are in excess. A fast growth of both PLL/ss-oligo phase and Q-dextran/oligo phase on the muscovite surface occurs at early stage. As the content of PLL reaches a certain value, the Q-dextran/ss-oligo phase is not stable. It undergoes a swelling and dissociation process to detach from the muscovite surface, followed by escaping of the majority of Q-dextran and less amount of ss-oligo (Figure 4A, 4B and Movie S5, S6). The PLL/ss-oligo phase still grows but at a much lower rate during the release of Q-dextran/ss-oligo phase. As more ss-oligo molecules are captured, the PLL/ss-oligo phase shrink into spherical shape (Figure 4C and Movie S6, S7). The amount of Q-dextran reaches minimum and mainly stays as the minor component of the PLL/ss-oligo phase.
FIGURE 4. Coacervation on muscovite surface via Q-dextran ← ss-oligo ← PLL. The concentrations of ss-oligo, Q-dextran and PLL are 1.5, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 2.0. (A) Time series of images showing the phase separation by FITC-PLL/(green), TRITC-Q-dextran (orange), Cy5-ss-oligo phase (red), and overlay of PLL and Q-dextran. Scale bars: 20 μm. (B) Time dependence of the normalized intensity of PLL and Q-dextran. (C) Time dependence of the normalized area of PLL and Q-dextran.
Route 2: PLL ← ss-oligo ← Q-dextran
PLL at 1.0 mg/ml is firstly applied on muscovite surface, followed by the addition of ss-oligo 5.0 min later. PLL and ss-oligo form spherical coacervate on the mineral surface at all the studied +/− ratios, with the heaviest coacervation occurring at +/− = 1.0 (Figure 5). Different from the behavior of Q-dextran/ss-oligo (Figure 1), the PLL/ss-oligo coacervate does not form an effective coating. We attribute it to the hydrophobicity of PLL/ss-oligo, which reduces its wetting ability on the hydrophilic muscovite surface.
FIGURE 5. Coacervation of PLL and ss-oligo on muscovite surface. The concentrations of ss-oligo were (A) 6.0 mg/ml, (B) 3.0 mg/ml, and (C) 1.5 mg/ml. The right corner in the selected panels shows the same image but intensified 10 times to reveal the phases. PLL at 1.0 mg/ml was applied 5.0 min earlier than ss-oligo. The green and red color denote the traces of FITC-PLL and Cy5-ss-oligo, respectively. Scale bars, 20 μm.
The addition of Q-dextran 15 min later initiates the formation of biphasic coacervates. The process is dependent on the concentration of ss-oligo or +/− charge ratio. In the case of 1.5 mg/ml ss-oligo, all ss-oligo molecules basically form coacervates with PLL in the first place. Because ss-oligo has the priority to form complex with PLL, the addition of Q-dextran does not generate prominent effect on the coacervation on muscovite surface as indicated by the fluorescence intensity (Figure 6B). However, the deviation of +/− ratio from unity to 2.0 results in a slow dissociation of larger size PLL/ss-oligo coacervates (indicated by the arrow in Figure 6A), together with the formation of spherical droplets floating in the solution (Figure 6A and 6B, Movie S8).
FIGURE 6. Coacervation on muscovite surface via PLL ← ss-oligo ←Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 1.5, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 2.0. (A) Time series of images showing the phase separation by (A) overlay of FITC-PLL/TRITC-Q-dextran (green/orange), (B) TRITC-Q-dextran (orange), and (C) Cy5-ss-oligo phase (red). Scale bars: 10 μm.
In the case of 3.0 mg/ml ss-oligo, the addition of Q-dextran increases the +/− ratio from 0.50 to 1.0, indicating that all the ss-oligo molecules form complex with PLL or Q-dextran. A fast growth of both PLL/ss-oligo and Q-dextran/ss-oligo occurs with time (Figure 7A and 7B). The Q-dextran/ss-oligo phase has the tendency to spread all over the muscovite surface (Movie S9), while the PLL/ss-oligo phase forms cluster structure via growth and fusion on the top of the continuous Q-dextran/ss-oligo phase (Movie S10). The decrease of the fluorescence intensity for all the three components in the ending stage (40.3 min) is probably caused by the mingling of the two phases.
FIGURE 7. Coacervation on muscovite surface via PLL ← ss-oligo ←Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 3.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 1.0. (A) Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green/orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm. (B) Time dependence of the normalized intensity of PLL and Q-dextran.
In the case of 6.0 mg/mL ss-oligo, the amount of ss-oligo is in excess before and after the addition of Q-dextran, indicating that all PLL and Q-dextran form complex or coacervates with ss-oligo. Similar as the phase separation in 3.0 mg/mL ss-oligo (Figure 7), the Q-dextran/ss-oligo phase spreads on the muscovite surface, while the PLL/ss-oligo phase forms clusters and then network on the top of the continuous Q-dextran/ss-oligo phase (Figure 8, Movie S11, S12). The borderline between the two phase are more distinct than that in 3.0 mg/mL ss-oligo.
FIGURE 8. Coacervation on muscovite surface via PLL ← ss-oligo ←Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 6.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 0.5. Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green/orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm.
Route 3: PLL/Q-dextran ← ss-oligo
The above results indicate that PLL and ss-oligo form stable coacervates under all the studied conditions, as demonstrated by their coincident fluorescence intensity, while Q-dextran/ss-oligo phase exhibits stronger capacity to coat the muscovite surface. The strong coating ability is attributed to the hydrogen bonding between Q-dextran and the muscovite surface (Jucker et al., 1997; Kwon et al., 2006). As the mixture of PLL and Q-dextran is firstly applied on the muscovite surface, followed by the addition of ss-oligo 5 min later, the phase separation process is determined by property of both PLL and Q-dextran.
In the case of 1.5 mg/ml ss-oligo, the charge ratio is 2.0, and polycations are in excess. Because Q-dextran is coated on the muscovite surface beforehand and concentrated, ss-oligo has the tendency to form coacervate with the Q-dextran coating (Figure 9, Movie S13). Besides being the minor component of the Q-dextran/ss-oligo phase (Figure 9B, Movie S14), PLL and ss-oligo mainly form spherical droplets which float in the solution.
FIGURE 9. Coacervation on muscovite surface via PLL/ss-oligo ←Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 1.5, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 2.0. (A) Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green/orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm. (B) Time dependence of the normalized intensity of PLL and Q-dextran.
In the case of 3.0 mg/ml ss-oligo, the ± charge ratio is unity. Similar situation is observed. Heavy coacervation of Q-dextran and ss-oligo occurs on the muscovite surface because of the coating beforehand (Figure 10). Meantime, PLL and ss-oligo form spherical droplets and stay mainly in the solution. In the later stage, however, a few PLL/ss-oligo droplets attach on the coated Q-dextran/ss-oligo phase (Figure 10 and Movie S15).
FIGURE 10. Coacervation on muscovite surface via PLL/ss-oligo ← Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 3.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 1.0. Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green/orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm.
In the case of 6.0 mg/ml ss-oligo, the +/− charge ratio is 0.5. All PLL and Q-dextran form coacervate with ss-oligo. The excess ss-oligo renders the Q-dextran/ss-oligo complex negative in nature, leading to weak Q-dextran/ss-oligo coating on the muscovite surface (Figure 11). PLL and ss-oligo mainly form spherical droplets in the early stage. Driven by gravity, the PLL/ss-oligo droplets fall on the muscovite surface and fuse into a “blanket”, covering most of the surface (Figure 11).
FIGURE 11. Coacervation on muscovite surface via PLL/ss-oligo ← Q-dextran. The concentrations of ss-oligo, Q-dextran and PLL are 6.0, 1.8, and 1.0 mg/ml, respectively. The final +/− ratio is 0.5. Time series of images showing the phase separation by overlay of FITC-PLL/TRITC-Q-dextran (green/orange), TRITC-Q-dextran (orange), and Cy5-ss-oligo phase (red). Scale bars: 20 μm.
Conclusion
The coacervates of PLL, ss-oligo, and Q-dextran exhibit different morphologies on the muscovite surface, which is highly dependent on the mixing order and the +/− ratios. It is the interplay of the four charged components (muscovite surface is treated as a polyanion) that determines the kinetics and the structure of the coacervates. Clearly, the charge density and hydrophobicity play key roles during the coacervation process. A comparison of the size and intensity of the coacervates (Figure 12) indicates that the Route 1, in which Q-dextran firstly interacts with the muscovite surface, generates the most distinct and versatile morphologies, including the spherical PLL/ss-oligo droplets on the surface at +/− = 2.0, floating PLL/ss-oligo droplets above the Q-dextran/ss-oligo blanket on the surface at +/− - = 1.0, and PLL/ss-oligo islands surrounded by the Q-dextran/ss-oligo sea at +/− = 0.5. Moreover, the kinetic path of each morphology is specific as indicated the supplementary movies. For example, the PLL-ss-oligo droplets on the surface is formed by forced dissociation of Q-dextran/ss-oligo phase, with Q-dextran being expelled and ss-oligo being captured (Figure 4 and Supplementary Movies S5∼S7). In Route two and Route 3, the coacervations on the muscovite surface are much weaker in most of the cases as indicated the fluorescence intensity. The coacervates at different charge ratios are also formed by similar path way: the intensity of both PLL and Q-dextran monotonously increase with time.
FIGURE 12. Coacervates on the muscovite surfaces at the ending stage as formed by different mixing orders and at +/− ratios of 0.5, 1.0, and 2.0. The green, red, and orange color denote the traces of PLL, ss-oligo and Q-dextran, respectively. Scale bars, 20 μm.
Formation of protocell with hierarchical structures and desirable biofunctions is the bridge from non-living matter to life. The morphologies (Figure 12) and the formation pathways of the coacervates on muscovite surface suggest that polysaccharide is probably the first biopolymer synthesized and accumulated on the mineral surface, which provides a “nest” for protein/peptide and DNA/RNA to from sub-compartments and to further develop advanced functions. Different from peptide and DNA, polysaccharides possess hydrophilic backbone and cannot be hydrolyzed in aqueous solution at normal conditions. On one hand, polysaccharides can reach much higher concentration in water. On the other hand, polysaccharides can retain large amount of water in the presence of hydrophobic biopolymers, such as peptides and lipids. More importantly, polysaccharides can wet the mineral surface in most of the cases, serving as a lubricate or protecting layer to ensure the integrity and mobility of other biopolymers. Taken together, our work provides a further understanding of the functions of biopolymers and their evolution pathway into protocell and possibly first life in the early Earth.
Data availability statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author
Author contributions
JC, YL, YL, and DL designed experiments. JC performed the experiments and analysed the results. ZL and QB assisted in analysing the results. JC and DL wrote the manuscript. All authors contributed to the article and approved the final manuscript.
Funding
Financial support of this work from the National Natural Science Foundation of China (21973002) is gratefully acknowledged. The measurements of LCSM were performed at the Analytical Instrumentation Center of Peking University. We acknowledge the assistance and support from PKUAIC (Yan Guan).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Supplementary Material
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Keywords: origin of life, protocell, coacervate, multiphase, minerals
Citation: Chen J, Liu Z, Li Y, Bai Q, Li Y and Liang D (2022) Coacervation of biopolymers on muscovite surface. Front. Soft. Matter 2:1054658. doi: 10.3389/frsfm.2022.1054658
Received: 27 September 2022; Accepted: 17 October 2022;
Published: 02 November 2022.
Edited by:
Lingxiang Jiang, South China University of Technology, ChinaReviewed by:
Yan Qiao, Institute of Chemistry (CAS), ChinaLiangfei Tian, Zhejiang University, China
Yaxun Fan, Institute of Chemistry (CAS), China
Copyright © 2022 Chen, Liu, Li, Bai, Li and Liang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Dehai Liang, ZGxpYW5nQHBrdS5lZHUuY24=