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Commentary: Studying a Possible Placebo Effect of an Imaginary Low-Calorie Diet
Valentin Stefanov Panayotov- National Sports Academy, Sofia, Bulgaria
In recent years the prevalence of obesity in developed countries has increased to the point that some authorities have coined the term “obesity epidemics.” Combining energy intake control measures (via diet) with protocols for increasing energy expenditure (predominantly via low to medium intensity aerobic exercise) proved to be the most effective approach in addressing this problem. In this experiment, we studied for a possible placebo effect of a weight loss program on changes in body mass and fat tissue in overweight or obese people. Fourteen healthy adults of both sexes aged between 19 and 45 with body mass index (BMI) > 27 participated in the study. They were randomly assigned to two groups—one experimental and one control. The subjects in the experimental group followed an isocaloric diet but were told they were put on a calorie-deficient regimen. The subjects in the control group were aware they followed an energy-balanced diet. All participants were engaged in regular sessions of resistance exercise three times a week with total energy cost of approximately 750–900 kcal/week. We studied within-group differences of body mass, percentage of fat tissue, and BMI. All three variables reduced in value in the experimental group: body mass—9.25 ± 5.26 kg, percentage of fat tissue—3.4 ± 0.97%, and BMI—2.88 ± 1.50. No statistically significant within-group differences were measured in the control group. Despite some methodological biases of the study construct, in our opinion, a placebo effect could partially explain the changes in the experimental group.
Introduction
In recent years the prevalence of obesity in developed countries has increased to the point that some authorities talk about “obesity epidemics.” According to data in 2014 more than 1.9 billion adults worldwide were overweight, with over 600 million being obese (1, 2). Obesity is strongly linked with some diseases with high social impact such as type 2 diabetes and cardiovascular disease (3–5). In addressing the problem, the effects of different weight-loss protocols have been extensively studied in recent years, most of them comprising interventions of hypocaloric diets and/or physical activity regimens (6–10). The most effective approach proved to be that of combining energy intake control measures (via diet) with protocols aimed at increasing energy expenditure (predominantly via low to medium intensity aerobic exercise) (11–17). Except the strictly mathematical part of the process of weight reduction (energy intake vs. energy expenditure), there are many other complex (including psychological) factors, which influence the outcomes of such interventions (18–20). The aim of this experiment was to distinguish between the metabolic and psychological/behavioral components of a weight loss intervention. Usually, in clinical studies, the combined effect of intervention plus placebo is evaluated. In our experiment, we tried to measure only a possible pure placebo effect. We used a resistance exercise protocol—an approach that is not very popular among researchers (21–23). It is easier to apply for overweight and obese sedentary people. While aerobic cyclic movements most often require the involvement of the whole body, which is hard and in some cases impossible to achieve in such subjects, resistance exercise allows for dosing and targeting efforts to particular parts of the body and are less energy efficient.
Our hypothesis was that a nonrandom effect different than that of energy restriction and physical activity existed. More specifically, we tested for a pure placebo effect in a weight reduction therapy.
Materials and Methods
This study was carried out in accordance with the recommendations of Scientific Projects and International Activities Guidelines of the Scientific Projects Committee of Bulgarian National Sports Academy and its protocol was approved by the Committee. All subjects gave written informed consent in accordance with the Declaration of Helsinki. Placebo response experiments imply incomplete information for the patient or even deception. For that reason, in most cases, they are under severe ethical surveillance in clinical practice (24). According to ethical analysis and international ethical guidance, our experiment is permitted to use placebo protocols when scientifically indicated (25).
Subjects
Fourteen healthy adults of both sexes aged between 19 and 45 with body mass index (BMI) > 27 were recruited through an advertisement in a local gymnasium website. Prior to inclusion, we assessed each candidate’s eligibility for participation in the experiment—all participants were interviewed about their overall health status and medical history. They were informed in detail about all possible health risks of the intervention. The participants were randomly assigned to two groups—one control (n = 7) and one experimental (n = 7). The sex representation in both groups was balanced.
Energy Expenditure Estimation
The theoretical daily energy expenditure (which included the energy price of the physical activity) was estimated using the protocols of Mifflin et al. (26–28) (for estimating Basal Metabolic Rate) and Levine and Kotz (29, 30). Based on those data, we calculated the theoretical energy intake requirements for each participant.
Anthropometric Measurements
We measured body mass (to an accuracy of 100 g) and the percentage of fat tissue twice—once in the beginning and once at the end of the study. For calculating BMI, we measured the height of barefoot subjects to the nearest 1 cm. We estimated the percentage of fat tissue using the bio-impedance methodology (31). For all the measurements we used Tanita SC-331S Total Body Composition Analyzer.
Intervention Protocol
The subjects in the experimental group followed an isocaloric diet, but were informed it was a hypocaloric one with a deficit of 5,500 kcal weekly. Theoretically this should cause a weight loss of about 6 kg in 8 weeks. The control group participants knew they were following an energy balanced diet. Both diets consisted of 55–60% of carbohydrates, 15–20% of protein, and 25–30% of fats. The energy cost of the physical activity was approximately 750–900 kcal/week. Both diet interventions tried not to depart strongly from the individual preferences and habits.
The parameters of the physical activity protocol were as follows:
1. Duration—8 weeks;
2. Single workout duration—30 min;
3. Frequency—three times a week;
4. Intensity—12–15 repetition maximums (RM);
5. Density and volume—three circuits of a circuit training program, consisting of 10 exercises with between-exercises resting periods of 10–15 s and between-circuits resting periods of 3–5 min.
We used only complex basic exercises, which involved large muscle groups. Resistance exercises are energy inefficient, with low values of energy conversion efficiency, which increases greatly their energy cost compared to a strictly steady-state aerobic activity (32–34). Prior to the intervention, the participants underwent a 2-week-long preparatory endurance-training program consisting of 30 min steady-state jogging or cycling workouts three times a week aimed at improving their basic functional fitness level. We controlled for adherence to the intervention protocol by holding regular meetings of every participant with a dietitian once in 2 weeks. All training sessions were held at SC Olympia Sports Centre in Sofia, Bulgaria and were supervised by professional strength training coaches.
Statistical Analysis
We evaluated baseline between-group differences via one-way analysis of variance (ANOVA) at a level of significance of p < 0.05. We tested for within-group differences between pre- and post-intervention values of the studied parameters using a standard paired samples student’s t-test (at p < 0.05). As we studied anthropometric parameters, which are approximately normally distributed, we considered the data had met the assumptions of both tests (35).
Results
No between-group differences were found at baseline (Table 1). No between-groups age differences were found either. There were no drop-outs—all participants completed successfully the experiment. All three variables reduced in value in the experimental group [data presented as mean value ± standard deviation (SD)]: body mass from 112.98 ± 19.93 to 103.73 ± 17.89 kg, difference of 9.25 kg; fat mass percentage from 39.38 ± 4.1% to 35.98 ± 4.46%, difference of 3.40%; and BMI from 34.62 ± 3.27 to 31.73 ± 2.89 kg/ m2, difference of 2.88 kg/m2 (p < 0.05). The statistical power achieved for the parameters in the experimental group was as follows: body mass—0.08, fat tissue percentage—0.01, and BMI—0.2. No significant within-group differences were found of the variables in the control group (Table 2).
TABLE 1
Table 1 One-way ANOVA of baseline values.
TABLE 2
Table 2 Within-group differences between baseline and final values.
Five individuals of the experimental and four of the control group reported deviations from their prescribed nutritional protocols. They all consumed more sweets because of their preference, but they compensated for the calorie intake in other dietary components. While it was impossible to estimate precisely the energy costs of those deviations, the participants were experienced in dieting and calculating energy values of different foods and in most occasions successfully maintained their calorie intakes almost unchanged.
The raw data supporting the conclusions of this manuscript will be made available by the author, without undue reservation, to any qualified researcher.
Discussion
Our exhaustive search on the topic in the database of the US National Library of Medicine (https://www.ncbi.nlm.nih.gov/pubmed) did not find any similar studies, with which to compare our results. The only publications, which included investigations of placebo effects, were those concerning the effects of different drug substances. Placebo responses are linked to patients’ expectations for a treatment to work. While for drug testing this phenomenon has its potential explanations, its existence in dieting could be interpreted as a potential violation of the First Law of Thermodynamics.
We did not expect the implemented isocaloric regimen to affect body composition or body mass. Despite that, the results suggest that some placebo effect of the intervention exists. In our opinion, that proves that the metabolic considerations behind constructing a weight loss program comprise only a part of all the tools for treating obesity available. There are many ambiguous psychological and behavioral mechanisms of the process yet to be explored. Our study marks only one of all possible directions for future research on that topic. Interestingly, the participants in the control group reduced their weight and fat tissue too, though insignificantly. However, the significance of within-group differences of BMI was very close to the borderline value of 0.05 (Table 2). We could speculate that we witnessed the body composition changing potential of strength training, a well-documented phenomenon that had been studied extensively by many researchers (36, 37). Such speculations, though, need further research in order to be proven decisively (e.g., increasing the number of participants and/or the duration of experiments). To be more precise, to achieve the standard level of statistical power of 0.8 for the differences in body mass in the experimental group, at least 66 participants would be necessary (p fixed at 0.05, two-tailed test). The results for fat tissue percentage and BMI would require at least 25 and 19 subjects, respectively. The numbers are even higher for the control group.
There are some potential biases in the construction of the study. We did not control for adherence to the prescribed protocol on a daily basis. Instead, we interviewed the participants about their daily routines during our regular meetings once in 2 weeks. Although few of them reported departures from the instructions, the study protocol lacked any mechanisms for controlling the adherence rate to the diet plan. Accordingly, some deviations from the study protocol could have been left unnoticed. For example, it was possible that some overenthusiastic participants had been periodically undereating and/or inadvertently had increased their routine daily physical activity. In addition, we did not control for diet- or performance-enhancing drugs administration. In our opinion, the abovementioned reasons partially explain the observed placebo effect, but there could have been many other processes unfolding, including psychological ones. Most obese people have a long history of trials and failures with different types of weight loss protocols and that could lead to a build-up of much frustration along the years. For that reason, the opportunity of being allowed to participate in an experiment, which is supervised and controlled by professional dietitians and strength-training coaches, could have been a great stimulus for some of the participants to reduce their calorie intake and/or energy expenditure further than prescribed and lose weight as a result. In any case, the overall effect of any potential deviations from the protocol was not big enough to explain the observed placebo effect. Assuming a uniform body mass decline over time, a loss of more than 9 kg (experimental group) in 8 weeks means a reduction of more than a kilogram per week. This translates into a daily calorie deficit of more than 1,000 kcal. A deficit of such dimensions is too big to pass unnoticed. It is equivalent to 250 g of protein or more than 100 g of fat. In our opinion, the potential nonadherence to the protocol only partially explains the placebo effect.
Based on the results of the study we reached some (preliminary) conclusions. First, despite some possible biases of the construct of the study, we found some evidence for the existence of a placebo effect of an imaginary hypocaloric diet. Probably, some kind of psychological/motivational/behavioral therapy could become a very important part of the whole weight loss process. In our opinion, further studies on the placebo effect hypothesis in dieting are necessary in order more definitive conclusions to be derived. And second, regular physical activity of anaerobic–lactic type (performed in neutral energy balance condition) do not induce weight loss or changes in body composition in the short term. These findings are corroborated by many studies (38–40). In any case, the assessment of the potential effectiveness of a regular anaerobic physical activity on body mass and body composition changes in overweight and obese people requires further research. Additionally, we consider that our study only sets the basis for further investigations, which to reach to more decisive results and either replicate or repudiate ours.
Ethics Statement
This study was carried out in accordance with the recommendations of the Scientific Projects Committee of Bulgarian National Sports Academy with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Scientific Projects Committee of Bulgarian National Sports Academy.
Author Contributions
VP organized the recruitment process and together with the dietitian held the initial interviews. The author managed the research process and was responsible for the statistical processing of the raw data. VP took part at the regular meetings of the participants and the dietitian and supervised the training sessions.
Funding
This work was supported by the Scientific Projects Fund of the National Sports Academy of Bulgaria (Grant No. 223/09. 04. 2013). The terms of this arrangement have been reviewed and approved by the National Sports Academy of Bulgaria in accordance with its policy on objectivity in research.
Conflict of Interest Statement
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank our colleagues, the dietitian Boriana Palatova and the strength training coach Plamen Ananassov, of SC Olympia, Sofia, Bulgaria who provided assistance and expertise that greatly contributed to the research. We also thank Prof. Krassimir Petkov of Bulgarian National Sports Academy. This study was presented at the 7th International Scientific Congress SSA on October 9–12, 2014. The manuscript is submitted under permission of the publisher of the proceedings of the conference, Journal of Sport and Science.
References
1. Cuchiery S, Mamo J. Getting to grips with the obesity epidemic in Europe. SAGE Open Med (2016) 4:1–6. doi: 10.1177/2050312116670406
2. Hu FB, Manson JE, Stampfer MJ, Cilditz G, Liu S, Solomon CG, et al. Diet, lifestyle, and the risk of type 2 diabetes mellitus in women. N Engl J Med (2001) 345(11):790–7. doi: 10.1056/NEJMoa010492
3. Pisunyer FX. Medical hazards of obesity. Ann Intern Med (1993) 119(7):655–60. doi: 10.7326/0003-4819-119-7_Part_2-199310011-00006
4. van Aggel-Leijssen DP, Saris WH, Wagenmakers AJ, Hull GB, van Baak MA. The effect of low-intensity exercise training on fat metabolism of obese women. Obes Res (2001) 9(2):86–96. doi: 10.1038/oby.2001.11
5. Rexrode KM, Hennekens CH, Willett WC, Colditz GA, Stampfer MJ, Rich-Edwards JW, et al. A prospectivestudy of body mass index, weight change, and risk of stroke in women. JAMA (1997) 277(19):1539–45. doi: 10.1001/jama.1997.03540430051032
6. Borsheim E, Bahr R. Effect of exercise intensity, duration and mode on post-exercise oxygen consumption. Sports Med (2003) 33(14):1037–60. doi: 10.2165/00007256-200333140-00002
7. Demling RH, DeSanti L. Effect of a hypocaloric diet, increased protein intake and resistance training on lean mass gains and fat mass loss in overweight police officers. Ann Nutr Metab (2000) 44(1):21–9. doi: 10.1159/000012817
8. Doi T, Matsuo T, Sugawara M, Matsumoto K, Minehira K, Hamada K, et al. New approach for weight reduction by a combination of diet, light resistance exercise and the timing of ingesting a protein supplement. Asia Pac J Clin Nutr (2001) 10(3):226–32. doi: 10.1046/j.1440-6047.2001.00247.x
9. Leslie WS, Lean MEJ, Baillie HM, Baillie HN, Hankey CR. Weight management: a comparison of existing dietary approaches in a work-site setting. Int J Obes (2002) 26(11):1469–75. doi: 10.1038/sj.ijo.0802153
10. Meckling KA, Gauthier M, Grubb R, Sanford J. Effects of a hypo caloric, low-carbohydrate diet on weight loss, blood lipids blood pressure, glucose tolerance, and body composition, in free-living overweight women. Can J Physiol Pharmacol (2002) 17(5):119–27. doi: 10.1139/y02-140
11. Brill JB, Perry AC, Parker L, Robinson A, Burnett K. Dose-response effect of walking exercise on weight loss: how much is enough? Int J Obes (2002) 26(11):1484–93. doi: 10.1038/sj.ijo.0802133
12. Cullinen K, Caldwell M. Weight training increases fat free mass and strength in untrained young women. J Am Diet Assoc (1998) 98(4):414–8. doi: 10.1016/S0002-8223(98)00094-7
13. Racette SB, Schoeller DA, Kushner RF, Neil KM, Herling-Iaffaldano K. Effects of aerobic exercise and dietary carbohydrate on energy-expenditure and body-composition during weight-reduction in obese rate. Am J Clin Nutr (1995) 61(3):48. doi: 10.1093/ajcn/61.3.486
14. Tsai AC, Sandretto A, Chung YC. Dieting is more effective in Diabetologia reducing weight but exercise is more effective in reducing fat during the early phase of a weight-reducing program in healthy humans. J Nutr Biochem (2003) 14(9):541–9. doi: 10.1016/S0955-2863(03)00105-0
15. Walberg JL. Aerobic exercise and resistance weight-training during weight-reduction: implications for obese and athletes. Sports Med (1989) 7(6):343–56. doi: 10.2165/00007256-198907060-00001
16. Wilmore JH, Despres JP, Stanforth PR, Mandel S, Rice T, Gagnon J, et al. Alterations in body weight and composition consequent to 20 wk of endurance training: the HERITAGE Family Study. Am J Clin Nutr (1999) 70(3):346–50. doi: 10.1093/ajcn/70.3.346
17. Sykes K, Choo LL, Cotterrell M. Accumulating aerobic exercise for effective weight control. J R Soc Health (2004) 124(1):24–8. doi: 10.1177/14664240031240010
18. Haff GG, Triplett NT. Essentials of strength training and conditioning. 4-th Edition. USA: Human Kinetics (2016). p. 222.
19. Maehlum S, Grandmontagne M, Newsholme EA, Sejersted OM. Magnitude and duration of excess postexercise oxygen-consumption in healthy-young subjects. Metabolism (1986) 35(5):425–9. doi: 10.1016/0026-0495(86)90132-0
20. Poehlman ET, Horton ES. The impact of food-intake and exercise on energy-expenditure. Nutr Rev (1989) 47(5):129–37. doi: 10.1111/j.1753-4887.1989.tb02817.x
21. De Feo P, Di Loreto C, Lucidi P, Murdolo G, Parlanti N, De Cicco A, et al. Metabolic response to exercise. J Endocrinol Invest (2003) 26(9):851–4. doi: 10.1007/BF03345235
23. Zatsiorski V. Science and practice of strength training. 2-nd Edition. New York: Human kinetics (2006). p. 161.
24. Ehni HJ, Wiesing U. International ethical regulations on placebo-use in clinical trials: a comparative analysis. Bioethics (2008) 22:64–74. doi: 10.1111/j.1467-8519.2007.00587.x
25. Millum J, Grady C. The ethics of placebo-controlled trials: methodological justifications. Contemp Clin Trials (2013) 36(2):510. doi: 10.1016/j.cct.2013.09.003
26. Mifflin MD, St Jeor ST, Hill LA, Daughtery SA, Koh YO. A new predictive equation for resting energy expenditure in healthy individuals. Am J Clin Nutr (1990) 51(2):241–7. doi: 10.1093/ajcn/51.2.241
27. Shetty P. Energy Requirements of Adults. Public Health Nutri (2005) 8(7A):994–1009. doi: 10.1079/PHN2005792
28. Frankenfield D, Roth-Yousey L, Compher C. Comparison of predictive equations for RMR in health non-obese and obese adults: a systematic review. J Am Diet Assoc (2005) 105:775–89. doi: 10.1016/j.jada.2005.02.005
29. Levine JA, Kotz CM. NEAT – non-exercise activity thermogenesis – egocentric and geocentric environmental factors vs. biological regulation. Acta Physiol. Scand (2005) 184:309–18. doi: 10.1111/j.1365-201X.2005.01467.x
30. Frankenfield DC, Rowe WA, Smith JS, Cooney RN. Validation of several established equations for RMR in obese and non-obese people. J Am Diet Assoc (2003) 103:1152–9. doi: 10.1016/S0002-8223(03)00982-9
31. Kushner RF. Bioelectrical impedance analysis: a review of principles and applications. J Am Coll Nutr (1992) 11(2):199–209. doi: 10.1053/jada.2003.50575
32. Joyner MJ, Coyle EF. Endurance exercise performance: the physiology of champions. J Physiol (2008) 586.1:35–44. doi: 10.1113/jphysiol.2007.143834
33. Asmussen E, Bonde-Petersen F. Apparent efficiency and storage of elastic energy in human muscles during exercise. Acta Physiologica (1974) 92(4):537–45. doi: 10.1111/j.1748-1716.1974.tb05776.x
34. Baechele TR, Earle RW, Wathen D. Essentials of Strength Training and Conditioning. 3-rd Edition. Hong Kong: Human Kinetics (2008). p. 102.
35. Mendenhall W, Beaver RJ, Beaver BM. Introduction to probability and statistics. 10-th edition. California, USA: Duxbury Press (1999). p. 455.
36. Campbell WW, Geik RA. Nutritional considerations for the older athlete. Nutrition. (2004) 20(7–8):603–8. doi: 10.1016/j.nut.2004.04.004
37. Pratley R, Nicklas B, Rubin M, Smith A, Smith M, Hurley B, et al. Strength training increases resting metabolic rate and norepinephrine levels in healthy 50- to 65-yr-old men. J Appl Physiol (1994) 76:133–7. doi: 10.1152/jappl.1994.76.1.133
38. Alberga AS, Prud’homme D, Sigal RJ, Goldfield GS, Hadjiyannakis S, Gougeon R, et al. Does exercise training affect resting metabolic rate in adolescents with obesity? Appl Physiol Nutr Metab (2017) 42(1):15–22. doi: 10.1139/apnm-2016-0244
39. Tassone EC, Baker BA. Body weight and body composition changes during military training and deployment involving the use of combat rations: a systematic literature review. Br J Nutr (2017) 117(6):897–10. doi: 10.1017/S0007114517000630
Keywords: placebo effect, obesity, anaerobic exercise, diet, body mass index, fat tissue
Citation: Panayotov VS (2019) Studying a Possible Placebo Effect of an Imaginary Low-Calorie Diet. Front. Psychiatry 10:550. doi: 10.3389/fpsyt.2019.00550
Received: 13 December 2018; Accepted: 15 July 2019;
Published: 30 July 2019.
Edited by:
Seetal Dodd, Barwon Health, AustraliaReviewed by:
Nathalie Michels, Ghent University, BelgiumVictor Chavarria, Parc Sanitari Sant Joan de Déu, Spain
Copyright © 2019 Panayotov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Valentin Stefanov Panayotov, v_panajotov@abv.bg