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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Abiotic Stress
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1533592
This article is part of the Research Topic Abiotic Stress Combination: Improving Resilience to Develop Climate-Smart Crops View all 4 articles

Identification and functional characterization of the MYB transcription factor GmMYBLJ in soybean leaf senescence

Provisionally accepted
Bao Guohua Bao Guohua 1Xu Xiao Xu Xiao 1Yang Jing Yang Jing 2Juanjuan Liu Juanjuan Liu 1Shi Tianran Shi Tianran 1Zhao Xiaoxuan Zhao Xiaoxuan 1Xuyan Li Xuyan Li 1*Shaomin Bian Shaomin Bian 1*
  • 1 Jilin University, Changchun, China
  • 2 Jilin Academy of Agricultural Sciences (CAAS), Changchun, Jilin Province, China

The final, formatted version of the article will be published soon.

    Leaf senescence is an important agronomic trait that significantly influences the 两端对齐 设置格式[Bsm]: 删除[Bsm]: analysis 删除[Bsm]: analysis 删除[Cui, Yuhai (AAFC/AAC)]: characterization 删除[Bsm]: associated with leaf senescence 删除[Cui, Yuhai (AAFC/AAC)]: in soybean 删除[Bsm]: Unclassified / Non classifié quality and yield of soybean. v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors are considered as crucial regulators governing leaf senescence, which can be utilized to improve agronomic traits in crops. However, our knowledge regarding the functional roles of soybean MYBs in leaf senescence is extremely limited. In this study, GmMYBLJ, a CCA1-like MYB, was identified and functionally characterized in leaf senescence. The GmMYBLJ protein is localized in the nucleus, and high accumulation of its transcriptswas observed in nodules and embryos. Noticeably, GmMYBLJ was highly expressed in soybean senescent leaves and transcriptionally induced by dark or NaCl treatment, which was confirmed by histochemical GUS staining analysis. Ectopic over-expression of GmMYBLJ in Arabidopsis not only led to earlier leaf senescence, less chlorophyll content and more MDA accumulation, but also promoted the expression of several several WRKY family transcription factors andsenescence-associated genes such as SAG12 and ORE1. Further investigation showed that over-expression of GmMYBLJ accelerated Arabidopsis leaf senescence under darkness and in responding to Pst DC3000 infection. Moreover, transgenic soybean plants over-expressing GmMYBLJ grew faster and exhibited accelerated senescence under salt stress. DAB staining analysis showed that GmMYBLJ conferred ROS accumulation in soybean hairy roots and Arabidopsis leaves. Collectively, our results provided useful information on the functional roles of GmMYBLJ in both aging-dependent and stress-induced senescence.

    Keywords: Soybean, GmMYBLJ, leaf senescence, Arabidopsis, dark, stress

    Received: 24 Nov 2024; Accepted: 06 Jan 2025.

    Copyright: © 2025 Guohua, Xiao, Jing, Liu, Tianran, Xiaoxuan, Li and Bian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xuyan Li, Jilin University, Changchun, China
    Shaomin Bian, Jilin University, Changchun, China

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