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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1518829
This article is part of the Research Topic Soybean Breeding for Abiotic Stress Tolerance: Towards Sustainable Agriculture View all 11 articles

Identification and functional validation of a new gene conferring resistance to Soybean Mosaic Virus strains SC4 and SC20 in Soybean

Provisionally accepted
Muhammad Muzzafar Raza Muhammad Muzzafar Raza Huiying Jia Huiying Jia Muhammad Khuram Razzaq Muhammad Khuram Razzaq Bowen Li Bowen Li Kai Li Kai Li *Junyi Gai Junyi Gai *
  • Nanjing Agricultural University, Nanjing, China

The final, formatted version of the article will be published soon.

    Introduction: Soybean Mosaic Virus (SMV) poses a serious threat to soybean production, often resulting in considerable yield losses or complete crop failure, particularly if infection occurs during early growth stages. While several SMV resistance genes have been identified, the genetic basis of resistance to certain strains remains poorly understood. Among the 22 SMV strains, SC4 and SC20 are considered pathogenic in Central China. Dominant genes resistant to SC4 (Rsc4) on Chr.14 in Dabaima and to SC20 (Rsc20) on Chr.13 in Qihuang-1 have been identified. Kefeng-1 is resistant to SC4 and SC20. This study aimed to determine whether the resistance to SC4 and SC20 in Kefeng-1 was identical and whether Rsc4 and Rsc20 in Dabaima and Qihuang-1 are also present in Kefeng-1 due to translocation.Methods and Results: Mendelian experiments using F 1 , F 2, and recombinant inbred lines (RIL 3:8 ) of Kefeng-1 (resistant) and NN1138-2 (susceptible) indicated a single dominant gene inheritance pattern in SC4 and SC20, respectively. Linkage mapping showed two loci genes for SC4 and SC20 in neighboring single nucleotide polymorphism linkage disequilibrium blocks (SNPLDB) marker regions of 253 kb and 375 kb, respectively, in Kefeng-1. Association between SNPs in possible gene regions of Kefeng-1 and resistance data showed SNP11692903 jointly as the most significant SNP, exhibiting the highest χ 2 value. By comparing SNP11692903 to possible gene sequences in the coding region, Glyma02g13380 was identified as a joint candidate gene. The results were validated using qRT-PCR, virus induced gene silencing (VIGS), and gene-sequence. Therefore, the two Mendelian genes on chromosome 2 in Kefeng-1 responsible for SC4 and SC20 resistance are unique genes, different from Rsc4 in Dabaima and Rsc20 in Qihuang-1. Conclusion: Hence, one gene is involved in resistance toward two SMV strains resistance. This result challenged our previous hypothesis of a single dominant gene responsible for resistance against a single strain and underscored the potential for using multiple resistance sources aimed at enhancing SMV resistance in breeding practices.

    Keywords: Mendelian inheritance, Soybean mosaic virus, Single nucleotide polymorphism, Resistance, linkage mapping

    Received: 29 Oct 2024; Accepted: 27 Nov 2024.

    Copyright: © 2024 Raza, Jia, Razzaq, Li, Li and Gai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Kai Li, Nanjing Agricultural University, Nanjing, China
    Junyi Gai, Nanjing Agricultural University, Nanjing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.