Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium
A Corrigendum on
Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle
by Takeya, M., Ito, S., Sukigara, H., and Osanai, T. (2018). Front. Plant Sci. 9:947. doi: 10.3389/fpls.2018.00947
In the original article, there were errors in Table 1 and Table S1 as published as a result of the incorrect calculation of kcat. We performed a re-calculation of and kcat values and corrected the kcat and kcat/Km values in Table 1 and Table S1; the corrected versions these tables and updated Table 1 footnote appear below.
The following sentence in the Results sub-section the Measurement of Kinetic Parameters contained an associated error: “SyMDH displayed approximately 1.7-fold (kcat) and 350-fold (kcat/Km) preferences for oxaloacetate reduction over malate oxidation and approximately 4.7-fold (kcat) and 89.5-fold (kcat/Km) preferences for NADH oxidation over NAD+ reduction (Table 1).” This sentence should have read: “SyMDH displayed approximately 1.7-fold (kcat) and 361-fold (kcat/Km) preferences for oxaloacetate reduction over malate oxidation and approximately 4.7-fold (kcat) and 90.5-fold (kcat/Km) preferences for NADH oxidation over NAD+ reduction (Table 1).”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Keywords: biochemistry, cyanobacteria, malate dehydrogenase, metabolic enzyme, TCA cycle
Citation: Takeya M, Ito S, Sukigara H and Osanai T (2021) Corrigendum: Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle. Front. Plant Sci. 12:699371. doi: 10.3389/fpls.2021.699371
Received: 23 April 2021; Accepted: 30 April 2021;
Published: 11 June 2021.
Edited and reviewed by: Peer Schenk, The University of Queensland, Australia
Copyright © 2021 Takeya, Ito, Sukigara and Osanai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Takashi Osanai, dG9zYW5haUBtZWlqaS5hYy5qcA==