Lior Doron
Na'ama Segal
Michal Shapira- Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel
Microalgae comprise a biodiverse group of photosynthetic organisms that reside in water sources and sediments. The green microalgae Chlamydomonas reinhardtii was adopted as a useful model organism for studying various physiological systems. Its ability to grow under both photosynthetic and heterotrophic conditions allows efficient growth of non-photosynthetic mutants, making Chlamydomonas a useful genetic tool to study photosynthesis. In addition, this green alga can grow as haploid or diploid cells, similar to yeast, providing a powerful genetic system. As a result, easy and efficient transformation systems have been developed for Chlamydomonas, targeting both the chloroplast and nuclear genomes. Since microalgae comprise a rich repertoire of species that offer variable advantages for biotech and biomed industries, gene transfer technologies were further developed for many microalgae to allow for the expression of foreign proteins of interest. Expressing foreign genes in the chloroplast enables the targeting of foreign DNA to specific sites by homologous recombination. Chloroplast transformation also allows for the introduction of genes encoding several enzymes from a complex pathway, possibly as an operon. Expressing foreign proteins in the chloroplast can also be achieved by introducing the target gene into the nuclear genome, with the protein product bearing a targeting signal that directs import of the transgene-product into the chloroplast, like other endogenous chloroplast proteins. Integration of foreign genes into the nuclear genome is mostly random, resulting in large variability between different clones, such that extensive screening is required. The use of different selection modalities is also described, with special emphasis on the use of herbicides and metabolic markers which are considered to be friendly to the environment, as compared to drug-resistance genes that are commonly used. Finally, despite the development of a wide range of transformation tools and approaches, expression of foreign genes in microalgae suffers from low efficiency. Thus, novel tools have appeared in recent years to deal with this problem. Finally, while C. reinhardtii was traditionally used as a model organism for the development of transformation systems and their subsequent improvement, similar technologies can be adapted for other microalgae that may have higher biotechnological value.
Introduction
Microalgae first attracted the attention of the biotech industry several decades ago as a potential platform for extracting natural products, with further improvements in their production relying on advanced technologies. Microalgae provide a manufacturing platform for the expression of foreign genes due to their quick growth under photoautotrophic conditions and their low cost of maintenance, as compared to land plants, mammalian cells, yeast, and bacteria. In addition, many algal species have developed unique metabolic pathways that produce compounds of commercial value (Priyadarshani and Rath, 2012; Rasala and Mayfield, 2014). Certain algal species, such as Dunaliella, are adapted for growth under extreme conditions, in this case high salinity (Ben-Amotz and Avron, 1972), thereby reducing the risk of contamination.
The plethora of high-value chemicals that can be extracted from both cyanobacteria and eukaryote microalgae include compounds used in the food industry, medicine, and cosmetics. The possibility of advancing the use of microalgae for future development of biofuel production and additional health-related products has also been examined. Microalgae have been exploited for the production of food additives, such as β-carotene (Varela et al., 2015) and astaxanthine, as well as long-chain polyunsaturated fatty acids (PUFAs; Borowitzka, 2013; Sharon-Gojman et al., 2015). Another line of industrial exploitation makes use of cell-wall sulfated polysaccharides, mainly for the cosmetics industry (Arad and Levy-Ontman, 2010). However, competition from cheaper parallel compounds extracted from macroalgae reveals the need for improvements to microlalgal systems. Efforts have long been directed at the industrial use of microalgal biomass for biofuel production, although the relatively high cost and low yield are of concern. Thus, biotechnological improvements combined with global market changes may, however, have major implications for the future use of microalgae as a biofuel source. Algae can also contribute in the development of novel and sensitive biosensors for environmental uses, such as monitoring pollutants in soil and water sources (Viji et al., 2014; Diaz et al., 2015). Finally, algae are an important component of the marine aquaculture, and can be used in ecological reef rehabilitation, especially in view of their ability to form symbiontic relationships with coral (Hagedorn and Carter, 2015).
Heterotrophic growth of microalgae is usually limited to bioreactors, whereas photoautotrophic growth can be carried out in open ponds. Still, the use of open ponds is restricted to extreme conditions (salt, temperature, pH) that allow exclusive growth of the organism of interest and avoid contamination by opportunistic organisms. This has been shown for Dunaliella, which is adopted to grow at high salt concentrations (Ben-Amotz and Avron, 1983), as well as for Spirulina, a photoautotrophic cyanobacteria that grows at high pH (Nolla-Ardevol et al., 2015).
In view of the above, genetic manipulations of microalga can lead to major changes in microalgal biotechnology-based industries. Such manipulations include the transfer of biosynthetic cascades into organisms that are suitable for growth in open ponds, or alternatively, adapting organisms that produce compounds of interest to grow under extreme conditions, thus reducing the risk of contamination. Recent developments in genome sequencing, combined with old and new systems for genetic manipulation, act synergistically to advance all aspects of microalgal research, both basic and applied. This review focuses on available systems and approaches for genetic manipulation of the chloroplast and nuclear genomes of microalgae, addressing unsolved issues, as well as points that await improvement.
Significant efforts have been invested in establishing tools that will allow for realization of the promise that algae hold for the production of high value bio-products, with most such tools having been originally developed for the model alga Chlamydomonas reinhardtii (Kindle et al., 1989). Transgene expression in Chlamydomonas was based on the accumulated identification of regulatory elements, such as promoters and untranslated regions (UTRs; Harris, 2009). Subsequently, successful nuclear transformation systems were also developed for ~25 microalgae species (see Tables 1, 2). In many cases, microalgal transformation resulted in stable expression of transgenes from either the nuclear or plastid genomes. The large amount of genomic and EST data from different algae contribute to the rich molecular toolbox currently available.
Table 1. List of selection markers and selection modes for chloroplast transformation.
Table 2. Commonly used nuclear control elements for constitutive or inducible transgene expression.
Chloroplast Transformation Systems
Technical Approaches Used for Chloroplast Transformation
Although stable transformation of microalgae was first developed for the chloroplast of C. reinhardtii, there are still fewer transformation systems that target the chloroplast, as compared to those targeting the microalgal nucleus. One great advantage of chloroplast transformation is that transgenes can be easily directed to integrate via homologous recombination, whereas nuclear transformation of microalgae usually results in random integration events. The development of the CRISPR-CAS9 system in microalgae may offer a solution for targeting transgenes into specific sites in the nuclear genome. The improvement of chloroplast transformation tools is also a dynamic field, as discussed elsewhere for microalgae (Purton, 2007; Purton et al., 2013) and higher plants (Bock, 2015).
The first stable transformation system for the chloroplast of C. reinhardtii was established using biolistic delivery. The foreign DNA was designed to rescue three mutants of the chloroplast atpB gene by homologous recombination of the transgenic marker into the target mutant strain and restore photosynthetic activity (Boynton et al., 1988). It was further shown that Chlamydomonas chloroplast transformation could be achieved by agitating cell wall-deficient cells with the DNA of interest in the presence of glass beads (Kindle et al., 1991; Economou et al., 2014; Rochaix et al., 2014). A chloroplast transformation system that was based on integration into the inverted repeat of the plastid genome using electroporation was also developed for Phaeodactylum tricornutum (Xie et al., 2014). Thus, similar technologies can be used for both chloroplast and nuclear transformations. The various markers for chloroplast transformation systems available today are summarized in Table 1.
Selection Systems
Selection Markers for Chloroplast Transformation Based on Photoautotrophic Growth
Chlamydomonas offers the advantage of being able to grow under non-photosynthetic conditions, using acetate as a carbon energy source. Thus, using non-photosynthetic mutants as recipient strains and recovery of their photosynthetic activity as a reporter system was used for reconstituting expression of the mutated atpB gene, encoding for ATP synthase (Boynton et al., 1988), and the tscA gene, encoding a small RNA that participates in trans-splicing of the psaA transcript (Goldschmidt-Clermont, 1991; Kindle et al., 1991).
Marker rotation is an approach that was originally aimed at examining whether the bacterial nifH gene from Klebsiella pneumoniae could replace the algal ChlL gene, which is responsible for chlorophyll biosynthesis in the dark. Both genes show a remarkable similarity in their domain structure, suggesting that nifH could replace ChlL for binding to a [4Fe–4S] cluster, thereby directly introducing the nitrogenase Fe protein into the Chlamydomonas plastome. In addition to using this approach for investigating the nitrogenase-like complex in the chloroplast, it could serve as a platform for plastid engineering into a functional nitrogenase-containing organelle. Accordingly, petB (cytochrome b6) was initially replaced by the selection marker aadA, and non-photosynthetic transformants were selected. A second round of transformation with the mutant strain restored the petB gene product and, moreover, introduced nifH (or uidA, encoding β-glucuronidase), allowing selection based on the ability to grow under photoautotrophic conditions (Cheng et al., 2005).
The use of FUD7 as a recipient strain offered a selection mechanism based on the ability of the transformed algae to grow under photoautotrophic conditions. FUD7 is a mutant that carries a deletion between exon 1 of psbA and the 5S gene. Thus, a cassette that reinstalls the open reading frame of psbA, along with a tagged gene of interest flanked by 3′UTR sequences, was established (Michelet et al., 2011).
Selection Markers for Chloroplast Transformation Based on Metabolic Enzymes
Metabolic selection of transformed cells provides a great advantage over the use of genes encoding for resistance to antibiotics and herbicides, as metabolic selection is considered to be more environmental friendly. Metabolic selection is common in yeast and animal cells, especially since many markers offer positive and negative selections. For instance, acetylornithine aminotransferase (ARG9) is a key enzyme in the metabolic pathway of arginine. It is encoded in the nucleus of C. reinhardtii, with the protein translocating into the chloroplast. A mutation in ARG9 resulted in an auxotrophic phenotype, such that the algal cells could only grow if arginine was added to the medium. The Arabidopsis thaliana ARG9 gene, known for its high A/T content that is typical of the chloroplast genome (Nakamura et al., 2000), was expressed in the plastid of a Chlamydomonas mutant strain that was originally deficient of ARG9 expression. The foreign ARG9 gene, now encoded by the chloroplast genome, was able to rescue the auxotrophic phenotype and restore arginine synthesis. This elegant approach created a metabolic selection system for chloroplast transformation in Chlamydomonas (Remacle et al., 2009).
Selection Markers for Chloroplast Transformation Based on Resistance to Antibiotics
Mutations in the sequence of the 16S (rrnS) and 23S (rrnL) rRNA that confer resistance to spectinomycin, streptomycin, kanamycin and erythromycin in C. reinhardtii (Harris et al., 1989) were previously used to establish an antibiotic-based selective marker that exchanged wild type RNA with the gene encoding resistant RNA (Newman et al., 1990). Later, resistance to spectinomycin was conferred by introducing the bacterial-derived aadA gene into the chloroplast genome, encoding aminoglycoside 3′ adenyl transferase. This gene is still the most frequently used marker for chloroplast transformations in algae and higher plants. The aadA-rbcL expression cassette was adapted and used to transform the genome of the Haematococcus pluvialis chloroplast (Gutiérrez et al., 2012).
An additional bacterial gene, aphA6, encoding aminoglycoside (3′) transferase that confers resistance to aminoglycoside antibiotics was used with Chlamydomnas reinhardtii. The combination of aadA with aphA6 extended the possibility for expressing multiple foreign genes in the chloroplast (Bateman and Purton, 2000). In 2013, the group of Stephan Mayfield constructed a cassette for Dunaliella tertiolecta chloroplast transformation. The construct that encodes both the gene of interest and the erythromycin esterase gene, ereB, was successfully introduced into D. tertiolecta cells via particle bombardment (Georgianna et al., 2013). A chloroplast-based expression system developed for the diatom P. tricornutum uses the chloramphenicol acetyltransferase (CAT) gene as a selection marker, as it confers resistance to chloramphenicol. This cassette promoted the expression of a foreign gene, along with the selection marker (Xie et al., 2014).
Finally, there is a strong demand for marker removal systems due to biotechnological concerns and commercial requests. Such a system was first established for Chlamydomonas by designing a dedicated cassette for promoting a recombination event that allows for removal of the selection marker, once the selection pressure is removed. The additional foreign gene in this cassette was maintained in the genome despite the removal of the selection gene marker because it was located outside of the sequence repeats that drive the recombination event (Fischer et al., 1996).
Selection Markers for Chloroplast Transformation Based on Resistance to Herbicides
A mutation in the fifth exon of the psbA coding region conferred resistance of Chlamydomonas cells to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), a herbicide that blocks the electron transfer pathway in PSII. Thus, DCMU resistance was initially used as a selection marker for understanding the occurrence of integration hotspots in the chloroplast genome (Newman et al., 1992). A different mutation in psbA also provides the cells with herbicide resistance to metribuzin (Przibilla et al., 1991).
The herbicide sulfometuron methyl (SMM) that inhibits growth of bacteria, yeast, algae and plants is commonly used as a selection marker in plants and algae. The target of SMM is the gene encoding acetohydroxyacid synthase (AHAS), an enzyme involved in the biosynthesis of branched amino acids. Since AHAS is encoded by the chloroplast genome of the red microalgae Porphyridium sp., it was found to be an ideal marker for chloroplast transformation. As such, a naturally occurring W492S mutation in the algal AHAS gene that confers resistance to SMM was used to establish a transformation system for Porphyridium sp. (Lapidot et al., 1999, 2002). During evolution, the gene encoding AHAS moved to the nucleus in land plants and green algae (Mazur et al., 1987), therefore allowing for its use as a selection marker in nuclear transformations in plants (Li et al., 1992; Ott et al., 1996) and green algae (Kovar et al., 2002). The AHAS-encoding gene was also later used as a transformation marker for the green microalgae Lobosphaera (Parietochloris) incisa, an algae with added value for biotechnological purposes (Grundman et al., 2012). In this case, the endogenous gene was first cloned, the W605S mutation in the active site of the enzyme that confers resistance to SMM was introduced, and the mutated gene was used for nuclear transformation.
Another chloroplast transformation system based on resistance to herbicides was developed for Platymonas subcordiformis using the bacterial bar gene encoding phosphinothricin acetyltransferase, which confers tolerance to glufosinate, or its ammonium salt, DL-phosphinothricin. This latter compound is the active ingredient in several herbicides, including the widely used Basta. The bar gene was also described as a selection marker in tobacco (Lutz et al., 2001). The use of this system in algae resulted in the development of a useful tool and a suitable selection system for this algae that is not sensitive to most commonly used antibiotics, such as spectinomycin, streptomycin or kanamycin (Cui et al., 2014).
Finally, as will be discussed below (see Section Biotechnological Exploitation of Microalgae), antibiotic and herbicide markers may raise biosafety concerns when genetic engineering is recruited for biotechnological purposes. Thus, selections involving metabolic markers that offer functional complementation of a missing endogenous gene product provide a clear advantage.
Regulatory Elements: Promoters, UTRs, and Codon Optimization
Efficient expression of foreign genes in algal chloroplasts is usually best obtained through the use of endogenous regulatory elements, derived from genes that are abundantly expressed. Thus, promoters that drive expression of the large subunit of ribulose bisphosphate carboxylase/oxygenase (rbcL), the D1 protein of the photosystem II reaction center (psbA), and the Y subunit of ATP synthase (atpA), were recruited for this purpose. The highest expression of soluble GUS was recorded for the atpA promoter and the 5′UTR (Ishikura et al., 1999). In another study, the atpA and psbD promoters and 5′UTRs were shown to drive the highest expression of a GFP reporter gene, when compared to the promoter and 5′UTRs of the rbcL and psbA genes. The presence of a 3′UTR derived from the different genes was required but had little effect on the accumulation of the transcript and foreign protein (Barnes et al., 2005). In later studies, the Goldschmidt-Clermont group demonstrated that the use of the psaA-exon1, its promoter and its 5′UTR increased foreign protein expression levels in the chloroplast. The authors also suggested that the variable expression observed among the different transformants that were derived from the same construct was related to the number and location of the recombination events (Michelet et al., 2011).
For a long time, foreign gene expression in the chloroplast suffered from low yields. With this in mind, limiting factors that could explain the low expression were extensively sought. It should be noted that gene expression in the chloroplast is tightly regulated at the translational level, and is less sensitive to transcriptional regulation. This was demonstrated in studies that inhibited the accumulation of specific chloroplast transcripts (by 90%) without affecting their translation rates. Gene dosage also proved to have little effect on the level of proteins encoded by these genes (Eberhard et al., 2002). The bottleneck in transgene expression from the chloroplast genome was addressed by the Mayfield group in 2007. They demonstrated that instead of targeting the expression cassette to the inverted repeats of the chloroplast genome, which yielded a low expression level of 0.5% relative to the total protein content in the cell, direct replacement of the psbA gene with a foreign coding region increased the production yield of the foreign protein to >5%. The authors suggested that the increased level of expression was due to a lack of competition encountered by regulatory factors (Mayfield and Schultz, 2004; Manuell et al., 2007).
Further improvements increased the level of transgene expression in the chloroplast of C. reinhardtii up to 20% of the total soluble protein content by focusing on optimization of codon usage and inhibition of ATP-dependent proteases. In addition, the toxicity of the transgene product may have a dramatic effect on its level of expression, and finally, large screens are usually required in order to isolate clones that efficiently express a transgene, possibly due to transformation-associated genotypic modifications that occur due to the random insertion of the foreign gene into the nuclear genome. Thus, the type of promoter and UTRs used are not the only factors that affect expression levels. The site of integration in the chloroplast, as well as accompanying random integration events in the nucleus, may also affect expression (Surzycki et al., 2009).
Chloroplast transformation systems that were developed for other algal species revealed differences with those developed for Chlamydomonas. For example, chloroplast transformation of Euglena revealed that the cassette containing the aadA gene flanked by the endogenous psbA promoter/5′UTR and 3′UTR generated drug resistant clones in which the DNA was maintained on a high molecular weight episomal element. Additional experiments using the aadA cassette introduced into the independently expressed Euglena gracilis psbK operon demonstrated proper splicing of the group III intron derived from the operon (Doetsch et al., 2001). Episomal elements carrying resistance genes are also common in non-photosynthetic kinetoplastids, such as Leishmania (Kapler et al., 1990), although the algal case describes a stable chloroplast episome.
Inducible Expression in the Chloroplast
Inducible expression offers considerable advantages, especially when the product of the transgene may interfere with cell growth or be toxic. NAC2 is essential for stabilization of the psbD mRNA (Kuchka et al., 1989), binding a unique target site in the psbD 5′UTR, and regulating the expression of any foreign gene under control of the psbD 5′UTR. The group of Rochaix introduced the NAC2 gene into Chlamydomonas cells under control of the cytochrome C6 promoter, which can be induced upon depletion of copper ions, by exposure to anaerobic conditions and in the presence of nickel ions (Merchant and Bogorad, 1987; Nickelsen et al., 1994; Quinn et al., 2002, 2003; Surzycki et al., 2007; Rochaix et al., 2014). Thus, manipulating the NAC2 gene to be induced by any of these conditions resulted in a similar pattern of regulation of a foreign gene that was driven by the psbD 5′UTR (Rochaix et al., 2014).
Nuclear Transformation
Nuclear expression of transgenes in microalgae offers several advantages, including targeting of foreign proteins for expression in organelles, such as the chloroplast, and protein glycosylation and/or additional post-translational modifications, as well as secretion (León-Bañares et al., 2004). These advantages are especially required for the exploitation of microalgae for industrial production for recombinant proteins, especially in view of the known difficulties to obtain efficient expression of foreign genes in microalgae (Eichler-Stahlberg et al., 2009). A collection of protocols for nuclear transformation, combined with the availability of several constitutive or inducible promoters and the availability of multiple selectable markers, offers a multitude of approaches for expression of transgenes in the nucleus.
Gene Delivery Methods
Generation of Protoplasts
The algal cell wall represents a physical barrier preventing the entry of foreign DNA through the cell membrane. Thus, many protocols for transformation rely on the use of protoplasts, which are cell wall-deficient. However, in many cases, generating protoplasts is a major bottleneck due to the diverse composition of the cell wall in different algae species, which, in many cases, remain poorly characterized (Popper and Tuohy, 2010). The Chlamydomonas cell wall is built from glycoproteins and contains little cellulose or chitin. As such, polysaccharide-degrading enzymes are ineffective, with protoplasts instead being generated by incubation with autolysins, namely hydroxyl-proline-specific proteases that are active during gametogenesis and mating (Jaenicke et al., 1987; Imam and Snell, 1988). Unlike Chlamydomonas, the cell wall in Chlorella consists of sugar polymers that can be degraded by sugar digesting-enzymes (Takeda, 1991). Thus, protoplasts of different Chlorella species can be generated by incubation with different sugar-degrading enzyme mixes (Braun and Aach, 1975; Yamada and Sakaguchi, 1982; Afi et al., 1996). Protoplasts from red and brown algae have been prepared using numerous cell wall-digesting enzymes extracted from marine mollusks or echinoderms (Liu et al., 1984; Cheney et al., 1986; Reddy et al., 2010).
Biolistic Delivery
The most frequently used method of gene delivery is biolistic transformation, also referred to as micro-projectile bombardment. This method utilizes DNA-coated gold or tungsten micro-particles that are delivered through a particle delivery system at high velocity into algal cells, surpassing the physiological barrier of the cell wall. Successful transformation by this approach have been reported for C. reinhardtii (Kindle et al., 1989), Dunaliella salina (Tan et al., 2005), Haematoccucs pluvialis (Steinbrenner and Sandmann, 2006), and diatoms (Dunahay et al., 1995; Apt et al., 1996; Falciatore et al., 1999; Zaslavskaia et al., 2000).
Glass Beads Method
A simple method that is often used for gene delivery is based on agitating protoplasts or cell wall-deficient mutants in the presence of glass beads, polyethylene glycol (PEG) and foreign DNA (Kindle et al., 1989). Successful transformations by this method were reported for C. reinhardtii (Kindle et al., 1989) and Chlorella ellipsoidea (Jarvis and Brown, 1991). In the case of C. reinhardtii, linearized plasmids usually yield higher transformation frequencies than supercoiled DNA when using glass beads or silicon carbide whiskers to mediate DNA entry (Kindle, 1990; Dunahay, 1993).
Electroporation
Applying an electric pulse is a commonly used method to introduce DNA into cells. This technique was used to transform microalgal protoplasts, cell wall-deficient mutants, and other thin walled algal cells. It was used to transform C. reinhardtii (Brown et al., 1991; Shimogawara et al., 1998; Yamano et al., 2013), D. salina (Walker et al., 2005b), Chlorella vulgaris (Chow and Tung, 1999), Scenedesmus obliquus (Guo et al., 2013), and Nannochloropsis sp. (Kilian et al., 2011). The use of cell wall-deficient strains improves transformation efficiency, with successful transformation using this methodology having been reported for Lobosphaera (Zorin et al., 2014). However, successful transformation without using cell wall mutants was also shown in P. tricornutum, either by electroporation (Niu et al., 2012; Zhang and Hu, 2014) or multi-pulse electroporation (Miyahara et al., 2013). In all these cases, the cells were grown without silica, which probably influenced cell wall structure. Overall, reducing cell wall thickness improves the transformation of microalga by electroporation.
Agrobacterium-Based Transformation of Microalgae
The highly popular system for nuclear transformation of land plants using Agrobacterium tumefaciens was also adapted for transformation of microalga, using the pCAMBIA transformation vector. Several microalgal species were transformed by Agrobacterium, including Chlamydomonas (Kumar et al., 2004; Pratheesh et al., 2014), as well as other algae of biotechnological value, such as H. pluvialis (Kathiresan et al., 2015), Schizochytrium (Cheng et al., 2012), Isochrysis galbana, and Isochrysis sp. (Prasad et al., 2014).
Nuclear Promoters and Control Elements
Gene Promoters
Efficient expression of foreign genes is achieved under the control of strong promoters. These are often derived from viruses, especially in land plants (Sanger et al., 1990), or from highly abundant endogenous genes, such as the small subunit of Rubisco (Goldschmidt-Clermont and Rahire, 1986). The use of a Cauliflower Mosaic Virus (CaMV35S) heterologous promoter that functions well in land plants gave inconsistent results when used to transform different algae species. Whereas, this promoter could drive the expression of reporter genes or chimeric genes in D. salina (Tan et al., 2005), Chlorella kessleri (El-Sheekh, 1999), and the dinoflagellates Amphidinium sp. and Symbiodinium microadriaticum (ten Lohuis and Miller, 1998; see Table 2) it gave contradictory results in C. ellipsoidea (Jarvis and Brown, 1991; Kim et al., 2002) and C. reinhardtii (Day et al., 1990; Blankenship and Kindle, 1992; Kumar et al., 2004; Diaz-Santos et al., 2013).
The use of strong endogenous promoters is recommended for nuclear transformation in algae, since heterologous promoters did not have an advantage, although they were active (Diaz-Santos et al., 2013). Among the endogenous regulatory elements, the RBCS2 promoter that drives expression of the small subunit of Rubisco, and PSAD (an abundant chloroplast protein of the Photosystem I complex) from C. reinhardtii drove efficient expression of transgenes (Stevens et al., 1996; Lumbreras et al., 1998; Fuhrmann et al., 1999; Fischer and Rochaix, 2001). Improved expression of transgenes was demonstrated in C. reinhardtii when the RBCS2 promoter was fused with the HSP70A (heat shock protein 70A) promoter, which acts as a transcriptional enhancer when placed upstream of the RBCS2 promoter (Schroda et al., 2000). Driving expression by the HSP70A/RBCS2 regulatory elements has become highly recommended for nuclear transformation of Chlamydomonas.
Other endogenous promoters have been used to drive the expression in other algal species, such as the promoter of the fucoxanthin-chlorophyll binding protein (FCP; Falciatore et al., 1999; Zaslavskaia et al., 2000; Poulsen et al., 2006). In the unicellular green alga Lobosphaera (Parietochloris) incisa, the endogenous RBCS promoter was used to drive expression of the ble gene, thus developing a platform for future successful engineering of this alga, which has great biotechnological potential due to its long-chain polyunsaturated fatty acid (PUFA) metabolism (Zorin et al., 2014).
Elements in the promoter region may occasionally have a negative effect on the expression of a transgene. Expression of the ble gene was silenced in 80% of transformants when it was driven from the RBCS promoter alone. However, when it was introduced under the control of the HSP70A/RBCS tandem promoter, silencing occurred in only 36% of transformed cells (Schroda et al., 2002). Modifying the HSP70A/RBCS2 promoter so that it contained four copies of the first RBCS2 gene intron between the HSP70A and RBCS2 promoters significantly increased the expression of the downstream gene (Rasala et al., 2012).
A recent study described the use of the constitutive endogenous promoter that drives expression of the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), for expression of the bacterial gene encoding bialaphos resistance (bar) and of the N-terminal fragment of human canstatin in D. salina (Jia et al., 2012).
A search for efficient endogenous promoters was performed by a “promoter trapping approach” in C. reinhardtii, by which a promoter-less selectable marker gene was randomly integrated into the nuclear genome (von der Heyde et al., 2015). The appearance of drug-resistant colonies indicated that the selectable marker gene integrated near a strong promoter. This approach, combined with the growing amount of species whose genomes have been sequenced, should enable the isolation of strong endogenous promoters in other algae as well.
Inclusion of Splicing Signals
Another point of concern is the inclusion of signals for RNA processing in the algal nuclei, since only processed mRNAs can exit the nucleus through the nucleopores. Thus, expression cassettes that contain an endogenous intron were prepared for improvement of transgene expression (Lumbreras et al., 1998; Fischer and Rochaix, 2001; Kovar et al., 2002).
Optimization of Codon Usage
The absence of tRNAs that are compatible with the codon usage of a given gene can dramatically affect expression of a transgene (Heitzer et al., 2007). Differences in codon usage were recorded between various microalgal species, as well as for the chloroplast and nuclear genomes of the same species (León-Bañares et al., 2004) and between organelles (Jarvis et al., 1992). Therefore, codon optimization of exogenous genes can significantly improve protein expression (Zaslavskaia et al., 2000; Franklin et al., 2002).
Inducible Expression in the Nucleus
The biotechnological exploitation of constitutive promoters for the effective expression of toxic compounds for industrial or pharmaceutical uses or for establishing silencing systems may occasionally be problematic. Hence, introducing inducible expression systems offers considerable advantages. Overall, the use of inducible systems is also attractive for large-scale production of recombinant proteins in microalgae, as these enable the cells to first reach an optimal concentration before expression of the transgene is initiated.
Nitrogen metabolism is based on the exploitation of ammonium ions. However, some algae can adsorb nitrate and convert it into ammonium with the help of nitrate reductase (Fernandez et al., 1989; Berges, 1997). The promoter of the nitrate reductase gene that is switched on and off in response to the presence of nitrate or ammonium ions (Poulsen and Kroger, 2005) was able to promote the inducible expression of a reporter gene in Cylindrotheca fusiformis (Poulsen and Kroger, 2005), C. reinhardtii (Schmollinger et al., 2010), C. ellipsoidea (Wang et al., 2004), D. salina (Li et al., 2007, 2008), Volvox carteri (von der Heyde et al., 2015), and Phaeodactylum triornutum (Niu et al., 2012).
The use of light for inducing expression in microalgae has long been pursued, initially by the use of the CABII-1 promoter that was shown to respond to changing light conditions and stimulate expression of the NIT1 gene under light in Chlamydomonas (Blankenship and Kindle, 1992). More recently, the light-regulated elements of the light-inducible protein (LIP) from Dunaliella was mapped to the flanking 400 bp upstream sequences of the LIP gene, which contains triplicates of light-responsible motifs. These were shown to induce expression of a heterologous reporter gene in Chlamydomonas (Park et al., 2013; Baek et al., 2016).
The metal-responsive CYC6 promoter that is repressed by copper was used for inducible gene expression in C. reinhardtii (Quinn and Merchant, 1995). The copper response element (CuRE) is responsive to both nickel and cobalt ions, and could be inhibited by EDTA (Quinn et al., 2003). Expression from this inducible system was improved by the addition of the first intron of the RBCS2 gene to the CYC6 promoter (Ferrante et al., 2011). In several marine and freshwater algae species, a control element located upstream of the B12-independent methionine synthase (METE) gene could repress the expression of a reporter gene following addition of B12 (Helliwell et al., 2014). The strong repressible nature and high sensitivity of the B12-responsive element can, therefore, be used as another promising gene expression tool for biotechnological applications.
Yet another element that was used to drive transgene expression is based on riboswitch biology (Mandal and Breaker, 2004). A riboswitch-regulated element based on the thiamine pyrophosphate (TPP) biosynthesis was identified in the THIC genes of a variety of organisms (Breaker, 2012), including Arabidopsis, where it affected THIC mRNA stability by alternative splicing of the primary mRNA (Bocobza et al., 2007; Wachter et al., 2007; Bocobza and Aharoni, 2014). This TPP riboswitch was later used for generating an inducible expression system in Arabidopsis (Bocobza et al., 2013) and in C. reinhardtii (Ramundo et al., 2013). Using the TPP system, Ramundo and colleagues were able to conditionally repress expression of the rpoA or rps12 genes in the chloroplast, affecting their organelle transcription or translation, respectively. Although riboswitch elements are common in prokaryotes, and have been used to generate inducible expression systems in bacteria (Neupert and Bock, 2009), the only riboswitch to be identified in higher eukaryotes was the TPP riboswitch, described above. The use of riboswitch-based regulation was recently used to develop a system for increasing expression of chloroplast genes in Arabidopsis, via an RNA amplification-based system that strongly improves the efficiency of riboswitches (Emadpour et al., 2015). A summary of the constitutive and inducible expression systems is given in Table 2.
Nuclear Selectable Marker Genes
Stable transformation is based on the use of a proper selection marker. These include genes that confer resistance to antibiotics or herbicides, and various metabolic enzymes that control growth under specific nutritional conditions. A collection of nuclear selective genes are listed in Table 3.
Table 3. List of selection markers and selection modes for nuclear transformation.
Selection of Nuclear Transformants Based on Auxotrophic Growth
Auxotroph genes can be used as selection markers for rescuing the phenotype of specific mutants that restrict growth under minimal conditions. The NIT1 gene, encoding nitrate reductase, promotes growth in the presence of nitrates as a nitrogen source (Fernandez et al., 1989), and was introduced for the nuclear transformation of the NIT1 mutant of C. reinhardtii (Kindle et al., 1989). This useful selection system was also applied for many other algal species (see Table 3). Another metabolic marker is the ARG7 gene, encoding argininosuccinate lyase. It was used to rescue arginine-requiring ARG7 mutants to prototrophy (Debuchy et al., 1989; Haring and Beck, 1997), and is commonly used for nuclear transformation of C. reinhardtii. The ARG7 mutant strain has been widely used for transformation of Chlamydomonas, mostly for basic research. A similar approach for use with other algae would be welcome, although a mutant that does not express argininosuccinate lyase must first be isolated. Another selection marker is based on NIC7, encoding quinolinate synthetase that is required for NAD biosynthesis. A plasmid carrying this gene could rescue a mutation in the C. reinhardtii NIC1 gene (Ferris, 1995; Lin et al., 2010), allowing growth in the absence of nicotinamide.
Selection of Nuclear Transformants Based on Resistance to Antibiotics
As with animal cell systems, numerous antibiotics genes have been successfully used as selection markers of microalgae. The ble gene that was originally isolated from Streptoalloteichus hindustanus, confers resistance to zeomycin and phleomycin (Sugiyama et al., 1994) and was used for generating transgenic clones of different algal species (see Table 3). The synthetic aminoglycoside adenyltransferase aadA gene confers resistance to spectinomycin and streptomycin (Svab and Maliga, 1993). It was originally used for chloroplast transformations but was further adopted for the nuclear system in Chlamydomonas (Cerutti et al., 1997b), and H. pluvialis (Gutiérrez et al., 2012). The aminoglycoside phosphotransferase genes aphVIII (aphH) from Streptomycesrimosus and aph7 from Streptomyces hygroscopicus confer resistance to paromomycin (Sizova et al., 2001) and hygromycin B, respectively. These were used to select drug-resistant algae in several species (see Table 3). The bacterial nptII gene encoding neomycin phosphotransferase was proven to be relatively inefficient in C. reinhardtii (Bingham et al., 1989; Hall et al., 1993). Codon optimization of the nptII gene, however, led to its improved function as a nuclear selection marker (Barahimipour et al., 2016). This selection marker was more successful when used for transforming other algal strains (see Table 3), as well as seed plants (Elghabi et al., 2011). The use of the cat gene, encoding CAT that confers resistance to chloramphenicol, has also been widely used (Table 3). Finally, the mutated version of the C. reinhardtii gene encoding ribosomal protein S14 (CRY1) confers resistance to emetine and cryptopleurine, and was adopted as a dominant selection marker (Nelson et al., 1994). Indeed, most antibiotic resistance genes have been successfully and routinely used for algal transformations (see Table 3). Nonetheless, their use as selection markers raises both health and ecological concerns for large-scale production in plants and microalgae.
Selection of Nuclear Transgenic Algae Based on Herbicide Resistance
The demand for production of plants and microalgae that are free of antibiotic resistance markers encouraged the use of herbicide-resistance markers, such as acetohydroxyacid synthase (ALS/AHAS) that confers resistance to sulfometuron methyl (SMM), phytoene desaturase (PDS) that generates resistance to the bleaching herbicide norflurazon, and glyphosate acetyltransferase (GAT) that contributes to resistance against glyphosate (Malik et al., 1989).
ALS/AHAS catalyzes the first step in the biosynthesis of the branched-chain amino acids, valine, leucine, and isoleucine (Kishore and Shah, 1988; Chipman et al., 1998). It is the target enzyme of the SMM herbicide that effectively inhibits growth of bacteria, yeast, plants, and algae. A mutant form of the gene encoding ALS/AHAS was used as a dominant selectable marker in the green algae Lobosphaera (Parietochloris) incisa (Grundman et al., 2012), based on experience gained from transformation of the chloroplast of the unicellular red alga Porphyridium sp. (Lapidot et al., 2002), and the green algae C. reinhardtii (Kovar et al., 2002).
PDS functions in the carotenoid biosynthesis pathway. Inhibition of this enzyme causes degradation of chlorophyll and the chloroplast membrane, as well as photo-bleaching of green tissues (Boger and Sandmann, 1998). Point mutations in PDS confer enhanced resistance to the bleaching herbicide norfurazon in the green microalgae H. pluvialis (Steinbrenner and Sandmann, 2006; Sharon-Gojman et al., 2015) and Chlorella zofingiensis (Huang et al., 2008; Liu et al., 2014). The use of GAT that confers increased tolerance to glyphosate is less recommended due to the reduced growth of GAT-expressing transformants in response to high concentrations of glyphosate, which were required to inhibit wild type cells (Bruggeman et al., 2014).
Targeting of Foreign Proteins to the Chloroplast
Targeting foreign proteins to sub-cellular compartments can affect their expression yield through protein folding, assembly and post-translational modifications. For example, the chloroplast provides an oxidizing environment that promotes the formation of disulfide bridges, and an abundance of chaperones that are required for the folding of soluble proteins after their import. The ability to properly fold and generate native disulfide bridges is fundamental for generating functional proteins and protein complexes, thus making the chloroplast an attractive organelle for the expression of recombinant proteins. This was demonstrated in C. reinhardtii by the production of the disulfide-containing Pfs25 protein from Plasmodium falciparum, which blocks transmission of the malaria parasite (Gregory et al., 2012). The importance of disulfide bond formation was also demonstrated by monitoring the expression and assembly of several fully active antibodies against pathogenic agents (Mayfield et al., 2003; Mayfield and Franklin, 2005).
Another advantage of organelle-specific expression is that targeting of nuclear-expressed proteins to chloroplasts can reduce the danger of proteolysis (Barnes et al., 2005; Doran, 2006; Mayfield et al., 2007). However, it still remains unclear why the ability to accumulate high levels of exogenous proteins in the chloroplast is heterogeneous. The plastid contains three large families of proteases, each of bacterial origin. These include the ATP-dependent Zn-metallo protease FtsH family (Adam et al., 2006; Liu et al., 2010), the ATP-independent Deg/HtrA family of serine endopeptidases (Huesgen et al., 2009; Sun et al., 2010) and the ATP-dependent serine-type Clp family (Adam et al., 2006). The chloroplast proteases can cleave exogenous proteins, as demonstrated in C. reinhardtii by the use of cyanide m-chlorophenylhydrazone (CCCP), which uncouples chloroplast and mitochondrial energy production. The presence of CCCP reduced degradation of the model protein VP28 from the White Spot Syndrome Virus three-fold expressed in the chloroplast, demonstrating that ATP-dependent proteases are involved in degrading this protein (Surzycki et al., 2009). Most chloroplast proteases are encoded in the nucleus, except for ClpP1. Thus, attempts to limit proteolysis of foreign genes expressed in the chloroplast can be controlled by knockdown technologies, such as RNAi, an approach that still requires further improvement in microalgae. Attempts to reduce expression of chloroplast-encoded ClpP by riboswitch control resulted in malfunctioning cells (Ramundo et al., 2014). However, deletion of the gene encoding ClpP protease from the chloroplast was achieved in A. thaliana (Zheng et al., 2006; Stanne et al., 2009), indicating that this approach could be feasible for microalgae as well.
Protein toxicity should also be considered when expression of foreign proteins in photosynthetic organisms is attempted. For example, the toxic effect of avidin, when expressed in the cytosol of transgenic tobacco plants, can be surpassed when this molecule is targeted to the vacuole (Murray et al., 2002). This also holds true for the cholera toxin-B subunit that is toxic to tobacco cells when expressed in the cytosol but not in the chloroplast (Daniell et al., 2001). Similar aspects should be considered when expressing foreign genes in microalgae.
Attempts to identify a consensus sequence shared by chloroplast and mitochondrial targeting peptides (cTPs and mTPs, respectively) were only partially successful (Habib et al., 2007; Huang et al., 2009). For this reason, data-driven machine learning techniques were developed (Schneider and Fechner, 2004), leading to the development of several programs, such as TargetP and Predotar (Emanuelsson et al., 2000; Emanuelsson, 2002; Small et al., 2004), that try to predict targeting peptides (TPs) for land plants. ChloroP is another program that predicts cleavage sites of TPs (Emanuelsson et al., 1999). These programs are based on the detection of an N-terminal targeting sequence that is shared between different targeted polypeptides. However, since green algae diverged from land plants over 725–1200 million years ago (Becker and Marin, 2009), their organelle import machineries, as well as their TPs, differ substantially from those of land plants. As such, most prediction programs are less reliable when used to predict the localization of algal proteins (Patron and Waller, 2007). With this in mind, a new algorithm, PredAlgo, that identifies cTPs/mTPs in green algae was developed. It is based on the accumulation of a large dataset from large- and small-scale proteomic studies of Chlamydomonas organelles (https://giavap-genomes.ibpc.fr/cgi-bin/predalgodb.perl?page=main; Tardif et al., 2012).
A recent study, based on the genomes of P. tricornutum and Thalassiosira pseudonana, provides a detailed analysis of TP motifs that target nuclear-encoded proteins to chloroplasts in diatoms and algae with secondary plastids of the red lineage (i.e., dinoflagellates, cryptophytes, and stramenopiles; Gruber et al., 2015). Organisms that belong to these phyla are of great interest to algae-based industry.
Targeting Transgene Products for Expression in Specific Organelles or Secretion
Targeting a transgene protein product for secretion is a common strategy for avoiding its degradation. A dedicated vector was designed in which luciferase expression in C. reinhardtii was greatly improved (up to 84%) by fusing the transgene with the previously identified secretion element of carbonic anhydrase (Lauersen et al., 2013a). This approach was further implemented for the production of secreted ice-binding protein, a protein of considerable industrial value (Lauersen et al., 2013b, 2015).
The targeting of nuclear transformation proteins to different cellular compartments is achieved by the addition of a TP-encoding sequence at the 5′-end of the transgene. TPs are recognized by the import machineries, which direct import into the proper organelle. Vectors that efficiently and specifically target transgene products to different compartments were generated (Rasala et al., 2014). Such vectors introduce TPs to the nucleus, mitochondria, chloroplast and ER using the nuclear localization signal (NLS) from simian virus 40, the N-terminal mTP from the nuclear gene encoding the alpha subunit of the mitochondrial ATP synthase, the N-terminal cTP from the photosystem I reaction center subunit II (encoded by psaD), and the ER-transit sequence from either BiP1 or ARS1, respectively. The TP that targets proteins to peroxisomes was also identified and was further shown to target a GFP transgene to the peroxisome of C. reinhardtii (Hayashi and Shinozaki, 2012).
In some cases, protein tagging is required for downstream applications, such as pull-down assays, immunoprecipitation, or protein purification. The introduction of such tags could interfere with the targeting signals. To prevent the obscuring of subcellular targeting signals, it is important to identify the TP of the native protein. In Chlamydomonas, endogenous proteins are usually tagged at their C-termini, since TPs are most frequently found at the N-terminus (Franzen et al., 1990; Patron and Waller, 2007).
Increasing the Efficiency of Transgene Nuclear Expression
While chloroplast transformation toolkits have been established for several microalgae, similar tools for nuclear-based protein expression remain under-developed, as for land plants. The reasons that lead to the low expression of transgenes from the nuclear genome could be varied, including the effects of position on integration events, epigenetic-derived transgene silencing, and difficulties related to variable codon usage systems (Jinkerson and Jonikas, 2015). Although the precise mechanism of insertion of DNA into the genome is unknown, it involves ligation of the transforming DNA at a site of double-stranded genomic DNA break, an event that occurs randomly throughout the genome with little sequence specificity, via the non-homologous end joining repair pathway (Kindle et al., 1989; Mayfield and Kindle, 1990; Zhang et al., 2014). In some cases, the transformed DNA integrates as a whole cassette, although truncated versions, fragments, or multiple cassettes that result from enzymatic cleavage can also be inserted (Zhang et al., 2014). Random integration events can sometimes result in “position effects,” in which the level of transgene expression is influenced by the surrounding genomic regions (Leon and Fernandez, 2007). It is generally accepted that screening of a large number of transformants in search of a high expressing clone is necessary (Hallmann, 2007).
Generation of Strains for Improved Transgene Expression
Despite the great advances made in developing systems for algal transformation, with C. reinhardtii serving as a fully sequenced model organism (Blaby et al., 2014), transgene expression using different methodologies and approaches remained limited until specific mutant strains capable of increased expression were isolated (Neupert et al., 2009). The basic assumption was that transgene expression could be affected by epigenetic processes, although the exact reasons remained unclear. To overcome this, a genetic screen was established in which wild type C. reinhardtii cells were subjected to random mutagenesis, and further screened for colonies that showed increased expression of the transgene. The screen used the ARG− selection system to select for transgenic algae, although another level of selection was introduced to screen for clones with increased expression. This was achieved by introducing CRY1-1, a ribosomal gene that confers resistance to emetine in a dose-responsive manner. As such, clones with increased CRY1-1 expression could grow in the presence of high emetine concentrations. Two clones, UVM4 and UVM11, were selected for increased transgene expression and are currently used for in Chlamydomonas (Lauersen et al., 2013b). It is tempting to examine whether a parallel system could be developed for other algae as well.
Improvement of Transgene Expression by Fusion with a Selection Marker
An expression cassette in which the codon-optimized GFP gene was fused to the ble selection marker increased GFP expression in C. reinhardtii (Fuhrmann et al., 1999). A further improvement was achieved by including the self-cleavable 2A peptide derived from the foot and mouth disease virus (Ryan et al., 1991) between the transgene and the ble selection marker. Its presence resulted in processing of the fused polypeptides to yield two independent proteins. This system led to an ~100-fold increase in the expression of several transgenes (Rasala et al., 2012, 2013).
The combination of using novel algal strains that promote increased expression of foreign genes, along with the sophisticated fusion between selection and target genes, opens a new era in transgene expression by the green algae Chlamydomonas, although similar developments with other algae are called for as well. The use of Chlamydomonas overexpressing strains (i.e., UVM11), combined with the codon optimization of the target gene, was shown to overcome the expression barrier of transgenes in Chlamydomonas. This approach led to the efficient expression of an HIV vaccine candidate, P24 (Barahimipour et al., 2016).
Expression of Transgenes from Episomes
A recent study reported the development of a nuclear episomal vector designed to introduce foreign DNA from E. coli into two diatom species, P. tricornutum and T. pseudonana, via conjugation. The vector contained a yeast-derived sequence that promoted its replication in these diatoms, even after antibiotic selection was eliminated. This episome was maintained as a closed circle at a copy number equivalent to the number of native chromosomes (Karas et al., 2015). This system offers great advantages in that it offers an easy method for introducing large DNA fragments into the host microalga, possibly promoting entry of several genes comprising a metabolic pathway. In addition, it is expected, although has yet to be shown, that expression of foreign genes from an episome would be less interrupted by epigenetic mechanisms or positional effects, since the DNA is not integrated into the chromosomal genome.
Gene Targeting in Algal Nuclei
Nuclear transformation of microalgae, in most studied cases, exhibited low frequency of homologous recombination (Sodeinde and Kindle, 1993; Nelson and Lefebvre, 1995), as was also observed with most photosynthetic organisms, except for mosses. Although occasional successful homologous recombination in the nuclei of Chlamydomonas had been reported in the past (Sodeinde and Kindle, 1993; Nelson and Lefebvre, 1995; Dawson et al., 1997; Hallmann et al., 1997; Minoda et al., 2004; Zorin et al., 2005), its frequency was too low for adaptation as a recommended technology. Efficient homologous recombination suitable for biotechnology applications was recorded in only two eukaryotic algae, namely Cyanidioschyzon merolae (Minoda et al., 2004) and Nannochloropsis sp. (Kilian et al., 2011).
Attempts to overcome difficulties that arise from positional effects and/or random integration of transgenes included the development of a Zn-finger nuclease system (Townsend et al., 2009) that can recognize and cleave a relatively long specific sequence, generating site-specific double-strand DNA breaks, thus modifying a gene of interest, as shown for the COP3 gene encoding a light-activated ion channel (Sizova et al., 2013). Another methodology that is currently being developed for microalgae is based on the CRISPR/Cas9 system, which uses a guide RNA that directs the Cas9 nuclease to restrict a specific sequence of DNA (Jinek et al., 2012). Components of the CRISPR/Cas9 system have recently been shown to function in a transient manner in C. reinhardtii, although the high toxicity of Cas9 prevented the successful recovery of stable colonies (Jiang et al., 2014). As such, further improvement of the CRISPR/Cas9 system is required.
Nuclear transgenic expression in microalgae is occasionally inefficient, possibly due to gene silencing (Cerutti et al., 1997a), or other reasons that are not yet fully understood. Still, even if all the elements required for optimal transcription and translation of transgene are provided, expression of exogenous genes can be very low or even non-existent, possibly due to gene silencing (Cerutti et al., 2011). Furthermore, expression of exogenous genes might be eliminated if transgenic algae clones are not maintained under constant selection conditions.
Gene Silencing in Algae Through the RNAi Machinery
The recent sequencing of several algal genomes has provided insight into the great complexity of these species, although algal physiology and metabolomics are still not fully resolved. Nonetheless, key components of the RNA-mediated silencing machinery, such as Dicer and Argonaute that can process double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs; Sontheimer and Carthew, 2005; Ghildiyal and Zamore, 2009; Voinnet, 2009; Fabian et al., 2010), have been found in many algae species, including C. reinhardtii (Schroda, 2006; Merchant et al., 2007; Kim and Cerutti, 2009), V. carteri (Ebnet et al., 1999; Cheng et al., 2006; Prochnik et al., 2010), D. salina (Sun et al., 2008; Jia et al., 2009), P. tricornutum (Bowler et al., 2008; De Riso et al., 2009), and E. gracilis (Iseki et al., 2002; Ishikawa et al., 2008). RNA-mediated silencing pathways have been studied in the unicellular green alga C. reinhardtii, where they are used as a reverse genetics tool for targeted knockdown of a variety of genes (Sineshchekov et al., 2002; Rohr et al., 2004; Soupene et al., 2004; Schroda, 2006). RNAi methodology was also used to examine the function of Aureochrome, a photoreceptor required for photomorphogenesis in stramenopiles. Silencing of the AUREO2 gene by introduction of dsRNA derived from the target gene induced the formation of sex organ primordia instead of branches, implicating it in the initiation of the development of a branch but not of a sex organ (Takahashi et al., 2007).
RNA-mediated silencing pathways have been studied in the unicellular green alga C. reinhardtii and used as a reverse genetics tool for targeted knockdown of a variety of genes (Sineshchekov et al., 2002; Rohr et al., 2004; Soupene et al., 2004; Schroda, 2006). The use of artificial miRNAs (amiRNAs), which mimic the structure of endogenous miRNA precursors was designed to enhance this approach (Molnar et al., 2009; Zhao et al., 2009). AmiRNAs can be designed by the microRNA designer platform (WMD3, http://wmd3.weigelworld.org/cgi-bin/webapp.cgi, active on March 14, 2016), which identifies suitable amiRNA candidates, based on optimal and specific hybridization properties, to target mRNA (Ossowski et al., 2008). Stable RNAi gene silencing was demonstrated in different microalgae as well, including V. carteri (Ebnet et al., 1999; Cheng et al., 2006), D. salina (Jia et al., 2009), P. tricornutum (De Riso et al., 2009), and E. gracilis (Iseki et al., 2002). Non-integrative dsRNA or siRNA was also used to trigger temporary RNAi in the green alga D. salina (Sun et al., 2008), E. gracilis (Ishikawa et al., 2008), and Vaucheria frigida (Takahashi et al., 2007).
Biotechnological Exploitation of Microalgae
Microalgae offer substantial potential for various biotechnological purposes, including production of animal food, aquaculture and marine agriculture, and biosynthesis of medical products, such as oral vaccines for animals (fish) and humans, high-value nutritional additives (e.g., polyunsaturated fatty acids, carotenoids, etc.), food dyes, and compounds used in the cosmetic industry and various other chemicals, in addition to possible future uses as a sources of energy. Moreover, microalgae-based technologies can be developed into water source treatments. These highly attractive goals justify the great efforts that the scientific community is investing in the development of molecular tools for the generation of transgenic microalgae.
Using Synthetic Biology for the Production of Commercial Added Value in Algae
In view of the difficulties in obtaining a high level of transgene expression in the cytoplasm of algae, the production of numerous therapeutic proteins was targeted to the chloroplast, including antibodies and proteins for use as oral vaccines. For example, a single-chain antibody was produced against the herpes simplex virus glycoprotein D (Mayfield et al., 2003; Mayfield and Franklin, 2005), and an IgG1 monoclonal antibody was generated against the anthrax protective antigen 83 (Tran et al., 2009). There were also attempts to use the chloroplast for producing oral vaccines against malaria (Gregory et al., 2012, 2013; Jones et al., 2013; Patra et al., 2015), Staphylococcus aureus (Dreesen et al., 2010), the white spot syndrome virus protein 28 (Surzycki et al., 2009), the P57 antigen bacterial kidney disease, (Siripornadulsil et al., 2007), and the VP1 antigen of foot and mouth disease virus fused to the Cholera toxin B subunit (Sun et al., 2003). Microalgae were exploited for the production of other immune reactive proteins, such as the human glutamic acid decarboxylase, a known auto-antigen in type 1 diabetes (Wang et al., 2008).
Heterologous therapeutic proteins were expressed not only in the chloroplast, but also in the nuclei of different algae, such as D. salina, Porphyridium sp., Nannocholorpsis oculata, and C. reinhardtii, to produce edible vaccines against the malaria parasite Plasmodium berghei (Dauvillée et al., 2010), the surface antigen of Hepatitis B virus (Geng et al., 2003) and for the production of food additives such as the xylanase growth hormone (Rasala et al., 2012; Georgianna et al., 2013) and Sep15, a selenium supplement (Hou et al., 2013). Algal-derived oral vaccines were shown to have a long shelf-life and their administration is injection-free. However, as the use of oral vaccines elicits mainly local immunity, their general efficacy must be carefully monitored.
Microalga have been harnessed to express enzymes of interest (Rasala et al., 2010, 2012), as well as for the potential production of biofuels (Georgianna and Mayfield, 2012), although the economical validity of this approach is still not satisfactory. Efforts were also targeted to enhancing the metabolic engineering of algae for enrichment of lipids, although this goal has yet to be reached (Dunahay et al., 1996).
Genetic manipulations could also help in adapting microalgae to different growth conditions. Attempts to convert autotrophic algae into heterotrophs relied on introducing the HUP1 gene form C. kessleri, which encodes for the hexose/H+ transporter that enables growth in the dark, into V. carteri (Hallmann and Sumper, 1996) and P. tricornutum (Zaslavskaia et al., 2001). Increased hydrogen production (by 150%) was obtained when the C. reinhardtii SMT6 mutant, which is a high H2-producing strain, was transformed with the HUP1 gene. This study established the possibility of improving the production of H2 from H20 and glucose (Doebbe et al., 2007). Increased hydrogen production was also obtained by a triple knockdown of the light harvesting complex proteins LHCMB1, 2 and 3 by RNAi, which resulted in a 180% increase in hydrogen production (Oey et al., 2013).
Cartenoid production from D. salina and H. pluvialis is also of great commercial interest, and is based on the natural production of β-carotene and astaxanthin. Manipulation of these species is possible, with increased expression of phytoene desaturase resulting in accelerated biosynthesis of astaxanthin. Production of an L504R mutant of phytoene desaturase resulted in increased production of astaxanthin (Steinbrenner and Sandmann, 2006). Furthermore, introducing the C. zofingiensis phytoene synthase, an enzyme that participates in the carotenoid biosynthetic pathway, into C. reinhardtii resulted in increased levels of violaxanthin and lutein (Cordero et al., 2011). These studies emphasize the great potential in manipulating metabolic pathways of high value products by engineering different algal species. Overall, microalgal systems hold great biotechnological potential that will surely be exploited in the future.
Biosafety Considerations
In the European Union, all genetically modified organisms (GMOs) or their products must receive approval before they can find their way to market. This practice also applies to genetically modified (GM) microorganisms. Thus, a guidance protocol for risk assessment of genetically modified microorganisms was prepared by the European Food Safety Authority (EFSA) in 2006 (http://www.efsa.europa.eu/sites/default/files/scientific_output/files/main_documents/374.pdf). The guiding protocols discriminate between the contained use of microalgae and their deliberate release into the environment, as further discussed in an OECD meeting on the Biosafety and Environmental Uses of Micro-Organisms (Wijffels, 2015). Contained use refers to closed conditions in which GM microorganisms are grown, stored, transported, destroyed and disposed, whereas deliberate release refers to GM microorganisms that are released in the environment without restricting their ability to spread. Contained growth can be performed in closed bioreactors (Chaumont, 1993), tubular reactors (Richmond et al., 1993), or polyethylene sleeves (Cohen et al., 1991). These technologies are designed to allow efficient growth and the harvesting of large quantities of microalgal biomass. Examples of deliberate release of GM microorganisms include their use for immunization of fish against various pathogens in open ponds, or when microalgae are employed for cleaning polluted water sources. Risk assessment is usually performed by testing the effects a GM microorganism has on humans, animals, plants and/or the environment, and comparing these effects with those of the conventional non-transformed variant. Risk assessment refers to the toxicity and allergenicity of the GM microorganism or its products, as well as the potential risk of horizontal gene transfer (HGT). To date, there are only a few reports on HGT in microalgae, and these refer mainly to viral-mediated HGT (Monier et al., 2009; Rosenwasser et al., 2014). However, to address this potential risk, even if it is very rare, the use of selection modes based on endogenous metabolic markers that complement a missing activity in a mutant recipient strain would be advantageous over the use of genes that confer resistance to herbicides or antibiotics. Removal of the selection markers is also recommended.
Conclusions and Future Perspectives
Despite occupying the base of the plant evolutionary tree, algae are distributed worldwide and grow in different climates, under normal and extreme conditions. As such, algae are known to provide a rich repertoire of chemicals and bio-molecules, including those of interest to biotechnological industries. Algae can also be harnessed as an important food source in the frame of aquatic agriculture. Finally, they provide a powerful resource for the production of bio-medical molecules, such as vaccines, antibodies and drugs (Cadoret et al., 2012). Another attractive achievement will enable the introduction of foreign genes that comprise a metabolic pathway that generates a valuable compound. A recent effort to express the complete carotenoid pathway toward enhanced astaxanthin formation was reported for Xanthophyllomyces dendrorhous (Gassel et al., 2014). However, these exciting possibilities are fully dependent on efficient expression of transgenes in microalgae. Indeed, this bottleneck must be addressed, since for occasional cases transgene expression had proven to be rather inefficient, for reasons that were not fully understood. Foreign genes can be targeted for expression in the chloroplast either by integration into the chloroplast genome or by their integration in the nuclear genome as fusion genes equipped with targeting signals that direct the import of the protein product into organelles. With regard to the general feasibility of expressing foreign proteins in microalgae, it appears that despite the considerable body of knowledge accumulated, inducing high expression of transgenes is difficult, possibly due to epigenetic mechanisms. Recent studies have made important improvements for overcoming this problem. Expanding the use of microalga for applied and basic research will benefit from the development of better protocols for gene knock-down by RNAi, as well as for successful deletion of specific genes by gene replacement. The recent revolution in genome editing provided by the CRISPR-Cas9 system should be adapted to microalgae in the coming years. Finally, the improvement of systems for inducible expression is also of great importance, since this will allow us to control the temporal expression of toxic molecules. Finally, rapid advancements in genome sequencing, now routinely performed for all organisms, will prove instrumental in advancing these goals in the near future.
Author Contributions
LS, NS, and MS all made literature surveys and participated in the writing of the review.
Funding
MS is incumbent of the Myles Thaler Chair in Plant Genetics. NS was supported by the Gazit-Globe fellowship.
Conflict of Interest Statement
The authors declare that the review article was written in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The reviewer, KG, and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review.
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Keywords: Chlamydomonas, chloroplast transformation of algae, diatoms, microalgae, nuclear transformation of algae, red microalgae, selection markers
Citation: Doron L, Segal N and Shapira M (2016) Transgene Expression in Microalgae—From Tools to Applications. Front. Plant Sci. 7:505. doi: 10.3389/fpls.2016.00505
Received: 12 November 2015; Accepted: 29 March 2016;
Published: 22 April 2016.
Edited by:
Steven James Burgess, University of Cambridge, UKReviewed by:
Ralf Alexander Wilhelm, Julius Kühn-Institut, GermanyKatrin Geisler, University of Cambridge, UK
Copyright © 2016 Doron, Segal and Shapira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Michal Shapira, shapiram@bgu.ac.il
†These authors have contributed equally to this work.