Joanna C. Chiu- Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, United States
Editorial on the Research Topic
Rising stars in chronobiology 2022
Chronobiology is the study of biological rhythms. The majority of studies in chronobiology to date have largely focused on circadian or 24-h rhythms, but there have been increasing interests in non-circadian biological rhythms, including seasonal rhythms (Wood and Loudon, 2014; Liams et al., 2019; Rohr et al., 2019; Abrieux et al., 2020) and circatidal rhythms (Kwiatkowski et al., 2023; Lin et al., 2023). The understanding of circadian rhythm has come a long way since Jean-Jacques d’Ortous de Mairan’s 1729 observations that the daily rhythm of leaf movement in Mimosa pudica plants persisted even when plants were placed in the dark (de Mairan 1729). His observations provided the first evidence to support the endogenous nature of circadian rhythms. Early evidence illustrating the genetic basis of circadian rhythm were documented centuries later when Bünning showed that crosses of Phaseolus bean plants with different period lengths with regard to leaf movement produced hybrid plants with intermediate period lengths, suggesting that circadian period length of leaf movement is an inherited trait (Bünning, 1932). An excellent account of the history of chronobiology has been compiled by the Society for Research on Biological Rhythms (SRBR) (https://srbr.org/about-chronobiology/chronohistory/).
The scope of circadian biology is immense and the impact of circadian rhythm is pervasive. Despite all the advances in the field in the past century, there is still much to discover. Given organisms from all domains of life inhabiting a range of ecological niche are found to possess circadian clocks, and cutting-edge genomic editing tools and high throughput genomic technologies now enable researchers to study rhythms in non-model species in more natural conditions, there are now increasing opportunities to study circadian rhythms that are unique and critical to understanding species-specific biology, e.g., swarming behavior in mosquitoes (Wang et al., 2021), social entrainment in colony insects (Siehler et al., 2021), and activity rhythms in Cnidarians (Kanaya et al., 2019). Conversely, the use of diverse models in the field of chronobiology will enable comparative analysis and formulation of broad principles governing circadian rhythm and its adaptive nature. Finally, the impact of chronobiology on biomedical sciences is hard to ignore, especially after 2017 when pioneering chronobiologists Michael Young, Michael Rosbash, and Jeffrey Hall, were awarded the Nobel Prize in Physiology and Medicine “for their discoveries of molecular mechanisms controlling the circadian rhythm.” In addition to established links between circadian disruption and many human diseases (reviewed in Manoogian and Panda, 2017; Masri and Sassone-Corsi, 2018; Acosta-Rodriguez et al., 2021; Lopez-Otin and Kroemer, 2021), chronobiologists and clinicians are increasing recognizing the potential of incorporating circadian medicine into healthcare and medical treatments (Ruben et al., 2019; Rasmussen et al., 2022; Kramer, 2023; Sole and Martino, 2023; Mangini et al., 2024; Zhu et al., 2024).
The field of chronobiology is growing rapidly; pioneering scientists have built a strong foundation and new investigators are infusing innovative ideas and creativity to accelerate new discoveries in basic principles of chronobiology while others are working towards leveraging circadian biology for precision medicine. The “Rising Stars in Chronobiology” Special Research Topic features the research of some of the up-and-coming investigators in the field of chronobiology. In Xin et al., Min-Dian Li and his group took a systems approach to define the circadian signatures in the adipose tissue of diet-induced obese mice and the impact of dietary intervention. Although the impact of malnutrition on liver circadian reprograming has been extensively studied (Eckel-Mahan et al., 2013; Guan et al., 2018; Martini et al., 2023), its impact on other peripheral tissues, e.g., adipose tissue, is less clear. Given different organs may respond to nutritional cues in distinct manner, this study provides new insights into how high fat diet can differentially alter circadian rhythms in different organ systems, potentially disrupting overall circadian synchrony.
In Verlande et al., Selma Masri and her group, who are at the forefront of circadian and cancer biology, demonstrated that a stable isotope 13C-glucose tracer, once injected in animals, can be monitored in real-time in exhaled breath to quantify glucose metabolism. They further validated this approach in models of diet-induced obesity and lung tumor progression, in which metabolic dysregulation is expected. Given circadian disruption is associated with metabolic dysregulation, this stable isotope technology will be valuable for studies in chronobiology.
In Wang et al., Wanhe Li collaborated with Nobel Laureate Michael Young to leverage Drosophila sleep mutants and investigate the impact of chronic sleep loss on rhythmic gene expression. They observed significant dampening in rhythmic gene expression in sleep mutants when compared to wild type flies. Interestingly, they found a subset of genes whose rhythmic gene expression was dampened in old flies and sleep mutants with chronic sleep loss, suggesting that aging may contribute to sleep loss induced disruption of circadian gene expression.
In Yuan et al., Swathi Yadlapalli and her group lamented that although subcellular localization of core clock proteins, such as CLOCK, are known to play a critical role in circadian clock function (reviewed in Patke et al., 2020), whether and how the spatiotemporal organization of clock mRNAs regulate circadian clock function have not been established. To address this question, they developed a streamlined single molecule fluorescence in situ hybridization (smFISH) protocol in combination with an automated machine-learning-based data analysis pipeline to quantify and assess localization of key clock mRNAs. They presented successful validation of their method by quantifying spatiotemporal expression of clock mRNA in Drosophila clock neurons.
Finally, in Anna et al., Nisha Kannan and her group leveraged the genetic tools in the Drosophila fly model to investigate the role of post-transcriptional mechanisms in regulating circadian rhythms. In particular, they characterized the role of miRNA-277 (miR-277) by downregulating its expression in multiple groups of clock neurons in the fly brain. They concluded that miR-277 plays a role in fine tuning the expression of Clk mRNA and in maintaining robust circadian rhythms.
As I am concluding this editorial, I would be remiss if I do not acknowledge the significant contribution of my late colleague Steve Brown to this Special Research Topic. I started this editorial assignment as a co-editor with Steve, who as many in the field of chronobiology know, was not only a brilliant scientist (Gachon, 2024), but a fierce supporter and mentor of junior colleagues and trainees. Steve was very excited to have the opportunity to highlight research by rising stars in the field. Tragically, Steve passed away in a plane crash on 14 December 2022. He now lived on in the memories of his family, friends, and others; he will be remembered by his colleagues in chronobiology for his joy for life and adventures, his innovation and scientific brilliance (e.g., Brown et al., 2005a; Brown et al., 2005b; Dallmann et al., 2012; Azzi et al., 2014; Gaspar et al., 2017; Brüning et al., 2019; Noya et al., 2019; Collins et al., 2020), and his generosity and kindness as a colleague, friend, and mentor.
Author contributions
JC: Conceptualization, Funding acquisition, Writing–original draft, Writing–review and editing.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The research in the laboratory of JC is funded by NIH R01 DK124068 and R56 DK124068.
Conflict of interest
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
Abrieux A., Xue Y., Cai Y., Lewald K. M., Nguyen H. N., Zhang Y., et al. (2020). EYES ABSENT and TIMELESS integrate photoperiodic and temperature cues to regulate seasonal physiology in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 117 (26), 15293–15304. doi:10.1073/pnas.2004262117
Acosta-Rodriguez V. A., Rijo-Ferreira F., Green C. B., Takahashi J. S. (2021). Importance of circadian timing for aging and longevity. Nat. Commun. 12 (1), 2862. doi:10.1038/s41467-021-22922-6
Azzi A., Dallmann R., Casserly A., Rehrauer H., Patrignani A., Maier B., et al. (2014). Circadian behavior is light-reprogrammed by plastic DNA methylation. Nat. Neurosci. 17 (3), 377–382. doi:10.1038/nn.3651
Brown S. A., Fleury-Olela F., Nagoshi E., Hauser C., Juge C., Meier C. A., et al. (2005b). The period length of fibroblast circadian gene expression varies widely among human individuals. PLOS Biol. 3 (10), e338. doi:10.1371/journal.pbio.0030338
Brown S. A., Ripperger J., Kadener S., Fleury-Olela F., Vibois F., Rosbash M., et al. (2005a). PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science 308 (5722), 693–696. doi:10.1126/science.1107373
Brüning F., Noya S. B., Bange T., Koutsouli S., Rudolph J. D., Tyagarajan S. K., et al. (2019). Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. Science 366 (6462), eaav3617. doi:10.1126/science.aav3617
Bünning E. (1932). Über die Erblichket der Tagesperiodizitat bei den Phaseolus-Blättern. Jahrb. Wiss. Bot. 77, 283–320.
Collins B., Pierre-Ferrer S., Muheim C., Lukacsovich D., Cai Y., Spinnler A., et al. (2020). Circadian VIPergic neurons of the suprachiasmatic nuclei sculpt the sleep-wake cycle. Neuron 108 (3), 486–499. doi:10.1016/j.neuron.2020.08.001
Dallmann R., Viola A. U., Tarokh L., Cajochen C., Brown S. A. (2012). The human circadian metabolome. Proc. Natl. Acad. Sci. U.S.A. 109 (7), 2625–2629. doi:10.1073/pnas.1114410109
Eckel-Mahan K. L., Patel V. R., De Mateo S., Orozco-Solis R., Ceglia N. J., Sahar S., et al. (2013). Reprogramming of the circadian clock by nutritional challenge. Cell 155, 1464–1478. doi:10.1016/j.cell.2013.11.034
Gachon F. (2024). Steve Brown’s legacy: tools to study the individual human molecular circadian clock and its regulation. Eur. J. Neurosci. Online ahead of print. doi:10.1111/ejn.16335
Gaspar L., Howald C., Popadin K., Maier B., Mauvoisin D., Moriggi E., et al. (2017). The genomic landscape of human cellular circadian variation points to a novel role for the signalosome. Elife 6, e24994. doi:10.7554/eLife.24994
Guan D., Xiong Y., Borck P. C., Jang C., Doulias P. T. T., Papazyan R., et al. (2018). Diet-induced circadian enhancer remodeling synchronizes opposing hepatic lipid metabolic processes. Cell 174, 831–842. doi:10.1016/j.cell.2018.06.031
Kanaya H. J., Kobayakawa Y., Itoh T. Q. (2019). Hydra vulgaris exhibits day-night variation in behavior and gene expression levels. Zool. Lett. 5, 10. doi:10.1186/s40851-019-0127-1
Kramer A. (2023). Time for circadian medicine. Acta Physiol. 238 (3), e13984. doi:10.1111/apha.13984
Kwiatkowski E. R., Schnytzer Y., Rosenthal J. J. C., Emery P. (2023). Behavioral circatidal rhythms require Bmal1 in Parhyale hawaiensis. Curr. Biol. 33 (10), 1867–1882.e5. doi:10.1016/j.cub.2023.03.015
Liams S. E., Lugena A. B., Zhang Y., Hayden A. N., Merlin C. (2019). Photoperiodic and clock regulation of the vitamin A pathway in the brain mediates seasonal responsiveness in the monarch butterfly. Proc. Natl. Acad. Sci. U.S.A. 116 (50), 25214–25221. doi:10.1073/pnas.1913915116
Lin Z., Green E. W., Webster S. G., Hastings M. H., Wilcockson D. C., Kyriacou C. P. (2023). The circadian clock gene bmal1 is necessary for co-ordinated circatidal rhythms in the marine isopod Eurydice pulchra (Leach). PLOS Genet. 19 (11), e1011011. doi:10.1371/journal.pgen.1011011
Lopez-Otin C., Kroemer G. (2021). Hallmarks of health. Cell 184 (1), 1929–1939. doi:10.1016/j.cell.2021.03.033
Mangini C., Zarantonello L., Formentin C., Giusti G., Domernie E. D., Ruggerini D., et al. (2024). Managing circadian disruption due to hospitalization: a pilot randomized controlled trial of the CircadianCare inpatient management system. J. Biol. Rhythms 39 (2), 183–199. doi:10.1177/07487304231213916
Manoogian E. N. C., Panda S. (2017). Circadian rhythms, time-restricted feeding, and healthy aging. Ageing Res. Rev. 39, 59–67. doi:10.1016/j.arr.2016.12.006
Martini T., Naef F., Tchorz J. S. (2023). Spatiotemporal metabolic liver zonation and consequences on pathophysiology. Annu. Rev. Pathol. 18, 439–466. doi:10.1146/annurev-pathmechdis-031521-024831
Masri S., Sassone-Corsi P. (2018). The emerging link between cancer, metabolism, and circadian rhythms. Nat. Med. 24 (12), 1795–1803. doi:10.1038/s41591-018-0271-8
Noya S. B., Colameo D., Brüning F., Spinnier A., Mircsof D., Opitiz L., et al. (2019). The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep. Science 366 (6462), eaav2642. doi:10.1126/science.aav2642
Patke A., Young M. W., Axelrod S. (2020). Molecular mechanisms and physiological importance of circadian rhythms. Nat. Rev. Mol. Cell Biol. 21, 67–84. doi:10.1038/s41580-019-0179-2
Rasmussen E. S., Takahashi J. S., Green C. B. (2022). Time to target the circadian clock for drug discovery. Trends biochem. Sci. 47 (9), 745–758. doi:10.1016/j.tibs.2022.04.009
Rohr K. E., Pancholi H., Haider S., Karow C., Modert D., Raddatz N. J., et al. (2019). Seasonal plasticity in GABAA signaling is necessary for restoring phase synchrony in the master circadian clock network. eLife 8, e49578. doi:10.7554/eLife.49578
Ruben M. D., Francey L. J., Guo Y., Wu G., Cooper E. B., Shah A. S., et al. (2019). A large-scale study reveals 24-h operational rhythms in hospital treatment. Proc. Natl. Acad. Sci. U.S.A. 116 (42), 20953–20958. doi:10.1073/pnas.1909557116
Siehler O., Wang S., Bloch G. (2021). Social synchronization of circadian rhythms with a focus on honeybees. Philos. Trans. R. Soc. Lond. B Biol. Sci. 376 (1835), 20200342. doi:10.1098/rstb.2020.0342
Sole M. J., Martino T. A. (2023). Circadian medicine: a critical strategy for cardiac care. Nat. Rev. Cardiol. 20, 715–716. doi:10.1038/s41569-023-00925-8
Wang G., Vega-Rodriguez J., Diabate A., Liu J., Cui C., Nignan C., et al. (2021). Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating. Science 371 (6527), 411–415. doi:10.1126/science.abd4359
Wood S., Loudon A. (2014). Clocks for all seasons: unwinding the roles and mechanisms of circadian and interval timers in the hypothalamus and pituitary. J. Endocrinol. 222, R39–R59. doi:10.1530/JOE-14-0141
Keywords: circadian rhythm, sleep loss, gene expression, adipose tissue, high-fat diet, diet-induced obesity, tumor, metabolism
Citation: Chiu JC (2024) Editorial: Rising stars in chronobiology 2022. Front. Physiol. 15:1412956. doi: 10.3389/fphys.2024.1412956
Received: 06 April 2024; Accepted: 11 April 2024;
Published: 25 April 2024.
Edited and reviewed by:
Rodolfo Costa, University of Padua, ItalyCopyright © 2024 Chiu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Joanna C. Chiu, jcchiu@ucdavis.edu