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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Translational Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1531524
This article is part of the Research Topic Pharmaceutical Biomaterials View all 26 articles

Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models

Provisionally accepted
Huaiyu Sun Huaiyu Sun Xuewei Li Xuewei Li Zhiqing Chen Zhiqing Chen Hongmei Meng Hongmei Meng *
  • First Affiliated Hospital of Jilin University, Changchun, China

The final, formatted version of the article will be published soon.

    Background: Lipids are important biomolecules involved in the formation of various biofilms.Seizures can cause changes in lipid metabolism in the brain. In-depth studies at multiple levels are urgently needed to elucidate lipid composition, distribution, and metabolic pathways in the brain after seizure.In this project, a cutting-edge targeted quantitative lipidomics study was conducted on the hippocampal tissues of 6 temporal lobe epilepsy rats and 6 normal rats. Accurate lipid quantification based on linear equations calculated using internal standard. The lipids were quantitatively and qualitatively analyzed by ultra-high performance liquid chromatography (UPLC) and mass spectrometry (MS).Results: A total of 21 lipid classes were identified. Among them, the most abundant were triacylglycerol (TG), phosphatidyl ethanolamine (PE-P) and fatty acids (FA). Cholesteryl ester (ChE) was the lipid exhibiting the most considerable difference between normal and epileptic samples.ChE was found to be the most significantly upregulated lipid, while FA was observed to be the most significantly downregulated lipid.Based on the absolute quantitative analysis of lipids in rat hippocampal specimens, the contents and change trends of different lipids were observed. Upregulation of ChE and dihydroceramide (DHCer) was observed, and analysis of the distribution changes contributed to elucidating the causes and possible molecular mechanisms of lipid accumulation in temporal lobe epilepsy. The results and methods described in this paper provide a comprehensive analysis of lipid metabolism in temporal lobe epilepsy and provide a new therapeutic target for the treatment of epilepsy.

    Keywords: targeted lipidomics analysis, Epilepsy, Temporal Lobe Epilepsy, molecular mechanism, Therapeutic target

    Received: 20 Nov 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Sun, Li, Chen and Meng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hongmei Meng, First Affiliated Hospital of Jilin University, Changchun, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.