Christina Mitsi
Javier Echeverría- Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile
Background: The genus Haplopappus Cass. [Asteraceae] comprises a large number of species distributed mainly in Chile and with various traditional medicinal uses.
Purpose: The present review addresses the botany, traditional uses, chemistry, biological and pharmacological activities of the genus, aiming to further potentiate the associated research and applications.
Study design and Methods: Literature data on the chemistry and bioactivity of the genus Haplopappus were mainly retrieved from digital databases such as SciFinder®, PubMed®, and Google Scholar®, as well as from the scientific journal publishers’ platforms linked with these databases.
Results and discussion: Although the majority of the botanical taxa of the genus Haplopappus has been understudied, available information is promising regarding its phytochemistry and bioactivity. A total of more than 400 compounds are present in different Haplopappus species, mostly terpenoids and phenolic compounds. Scientific literature supports various health promoting effects of Haplopappus extracts and isolated compounds, principally their effect against human pathogenic bacteria and their high antioxidant capacity. The existing limitations highlighted hereby are mainly associated to the lack of modern investigation regarding a wider number of Haplopappus species and chemical compounds, as well as to the absence of in vivo bioactivity results and clinical trials.
Conclusion: Scientific literature supports the ethnopharmacological, phytochemical and bioactive potential of the genus Haplopappus, however the aforementioned limitations need to be addressed in order to further promote and broaden both scientific research and future applications and uses.
1 Introduction
Haplopappus Cass. (Asteraceae (Compositae) - Astereae - Machaerantherinae), is a strictly endemic botanical genus of southern South America, distributed in Chile, with some species also present in Argentina (Klingenberg, 2007). The vernacular name ‘bailahuén’ (‘baylahuén’ or ‘vaila-huen’) has been mainly attributed to the species Haplopappus baylahuen Remy, although the other species of the genus are commonly referred to using the same name (Vogel et al., 2007).
The different species of the genus Haplopappus, although used without differentiation in terms of botanical taxa, are of high ethnopharmacological importance and form part of the longstanding traditional medicines of the Andean peoples. In Chile, where the genus is mainly distributed, its species have been widely used in all territory, from the Aymara communities in the north to Mapuche communities in the south, and in big cities by different social groups (Hoffmann et al., 1992). Bailahuén is used at the prevention and/or treatment of various human and animal pathologies, mainly -but not exclusively-associated to gastrointestinal ailments and wound healing (Muñoz et al., 1981; de Mösbach, 1992; Hoffmann et al., 1992). Alongside its traditional use, H. baylahuen is also included in the German Homeopathic Pharmacopeia as a herbal medicine against fatigue and low blood pressure, although its use is considered limited (Arzneibuch, 2006; Vogel et al., 2007).
Regarding its commercialization, it is reported that its production in Chile is exclusively based on the collection of plant material in the wild, which, in most cases, is realized by non-trained individuals (Vogel et al., 2007). Furthermore, in the same study it is highlighted that the 80% of bailahuén commercial samples correspond to Haplopappus multifolius, probably due to the fact that this species is distributed in the Metropolitan Region of Santiago, where the companies that commercialize the plant material at a national and international level are also located. The over-exploitation of H. multifolius, along with inadequate collection practices, have led to the species being recently included in The IUCN Red List of Threatened Species as Near Threatened (Plummer, 2022).
In this context, despite its high botanical diversity and the rich ethnopharmacological background of the genus Haplopappus, both scientific investigation and commercial use is often limited to a few botanical taxa, while in many cases the traditional knowledge associated with the genus is not taken into consideration, thus hindering unravelling the full phytochemical and bioactive potential of the genus.
Thus, the present article aims to present a comprehensive review of the current state of knowledge regarding the botany, traditional uses, chemistry, biological and pharmacological activities of the genus Haplopappus in an attempt to underline its phytochemical uniqueness, elucidate its bioactive potential, and highlight future research opportunities.
2 Methods
Literature data on the chemistry and bioactivity of the genus Haplopappus were mainly retrieved from digital databases such as SciFinder®, PubMed®, and Google Scholar®, as well as from the scientific journal publishers’ platforms linked with these databases. The search strategy included the scientific name of the genus, excluding the species presently classified in other genera, i.e., Ericameria Nutt., Grindelia Willd., Gundlachia A.Gray, Isocoma Nutt., Notopappus L. Klingberg (Klingenberg, 2007; POWO, 2024). All publications in peer-reviewed journals until May 2024 were considered. The chemical compounds present in the raw materials were classified according to their pathway and superclass (Supplementary Table S1; Figures 1–11) using the NPClassifier tool (Kim et al., 2021).
Figure 1. Chemical structures of miscellaneous compounds identified from Haplopappus species.
Figure 2. (A) Chemical structures of acyclic monoterpenes identified from Haplopappus species. (B) Chemical structures of monocyclic monoterpenes identified from Haplopappus species. (C) Chemical structures of aromatic monocyclic monoterpenes identified from Haplopappus species. (D) Chemical structures of bicyclic monoterpenes identified from Haplopappus species. (E) Chemical structures of tricyclic monoterpenes identified from Haplopappus species.,
Figure 3. (A) Chemical structures of acyclic sesquiterpenes identified from Haplopappus species. (B) Chemical structures of monocyclic sesquiterpenes identified from Haplopappus species. (C) Chemical structures of bicyclic sesquiterpenes identified from Haplopappus species. (D) Chemical structures of tricyclic sesquiterpenes identified from Haplopappus species.,
Figure 4. (A) Chemical structures of diterpenes labdane-type1 identified from Haplopappus species. (B) Chemical structures of diterpenes labdane-type2 identified from Haplopappus species. (C) Chemical structures of diterpenes labdane-type3 identified from Haplopappus species. (D) Chemical structures of diterpenes friedolabdane-type identified from Haplopappus species. (E) Chemical structures of diterpenes clerodane-type identified from Haplopappus species. (F) Chemical structures of miscellaneous diterpenes identified from Haplopappus species., ,
Figure 5. Chemical structures of triterpenes and triterpenoids identified from Haplopappus species.
Figure 6. Chemical structures of meroterpenes identified from Haplopappus species.
Figure 7. Chemical structures of steroids identified from Haplopappus species.
Figure 8. Chemical structures and substitution patterns of flavonoids identified from Haplopappus species.
Figure 9. Chemical structures and substitution patterns of coumarins identified from Haplopappus species.
Figure 10. Chemical structures of benzoic acid derivatives identified from Haplopappus species.
Figure 11. Chemical structures of cinnamic acid derivatives identified from Haplopappus species.
3 Botany and distribution
The genus Haplopappus Cass. (Asteraceae - Astereae - Machaerantherinae) is a strictly endemic genus of South America and its species are mainly distributed in Chile and, to a lesser extent, Argentina (Klingenberg, 2007; Rodriguez et al., 2018; Zuloaga et al., 2019; García et al., 2024).
According to the latest taxonomic studies of the genus and after the separation of numerous, mainly North American, species that formed the genus Notopappus L. Klingenberg, the genus Haplopappus consists of 70 specific and intraspecific taxa (Table 1) and is subdivided into three subgenera (Haplopappus subgen. Haplopappus, H. subgen. Grindelioidae Klingenberg, and H. subgen. Baylahuen Klingenberg) and five sections: Haplopappus sect. Haplopappus, H. sect. Gymnocoma Nuttall, H. sect. Grindelioidae Klingenberg, H. sect. Chromochaeta Candolle, and H. sect. Leiachaenium Candolle (Klingenberg, 2007; Garcia et al., 2018; García et al., 2024). Haplopappus taxonomy is mainly based on morphological traits, due to the limited phylogenetic data available up to date (García et al., 2024). In general, Haplopappus species are shrubs or subshrubs, with aerial parts that bear glandular trichomes, usually yellow florets, and numerous pappus bristles (Klingenberg, 2007; García et al., 2024).
Table 1. Scientific names and distribution of reported Haplopappus species (Klingenberg, 2007; Garcia et al., 2018; García et al., 2024).
4 Phytochemistry
Available scientific literature provides relevant information on the phytochemistry of the genus Haplopappus. However, it must be mentioned that this information refers to only 28 species and subspecies of a total of 70 taxa (Table 1), thus highlighting the largely understudied potential of the genus Haplopappus and stressing the need to further investigate its phytochemistry. Moreover, of these 28 taxa for which scientific evidence is available, for the 24 there are less than 35 compounds reported per taxa, whereas the remaining four species are associated to a higher -yet still rather diverse-number of reported compounds, i.e., H. foliosus (n = 146), H. velutinus (n = 59), H. chrysanthemifolius (n = 52), H. bustillosianus (n = 40).
Regarding the type of metabolites reported in Haplopappus species, more than 400 different molecules have been detected in various plant parts of the studied taxa. However, the number of reported compounds per chemical group is highly diverse, to an extent that it raises the question of whether this variability can be solely attributed to differences at a plant metabolic level or it can also be associated with a focus of scientific research towards certain groups of metabolites, e.g., terpenoids and phenolics. Indeed, products of the terpenoid metabolic pathway are by far the most abundant group of molecules reported in the genus Haplopappus, including more than 200 compounds, i.e. 54 monoterpenoids (abbreviated as Mon in compound codification used in the present review), 60 sesquiterpenoids (Sqt), 107 diterpenoids (Dit), five triterpenoids (Tri), one meroterpenoid (Mer) and two steroids (Str). The second most abundant group of reported compounds includes flavonoids (Flv; flavonols, n = 46; flavones, n = 20; flavanones, n = 8; flavanonols, n = 11) and other products of the metabolic pathway of shikimic acid, i.e., coumarins (Cum, n = 16), benzoic (Ben, n = 3) and cinnamic (Cin, n = 12) acid derivatives. Other compounds reported in the genus Haplopappus include alkanes (Ala, n = 29), alkenes (Ale, n = 4), alkynes (Aly, n = 1), alcohols (Alc, n = 5), ethers (Eth, n = 1), aromatic hydrocarbons and derivatives (Arh, n = 10), aldehydes (Ald, n = 8), ketones (Ket, n = 7), esters (Est, n = 8), furanones (Fur, n = 1), lactones (Ltn, n = 2) and lactams (Ltm, n = 1).
The aforementioned compounds as classified per chemical group are detailed in Figures 1–11 and Tables 2–4, and Supplementary Table S1, while their distribution among the studied Haplopappus taxa is presented as follows.
Table 2. Substitution pattern of flavonols and flavones reported in species of the genus Haplopappus.
Table 3. Substitution pattern of flavanones and flavanonols reported in species of the genus Haplopappus.
Table 4. Substitution pattern of coumarins reported in species of the genus Haplopappus.
4.1 H. angustifolius (DC.) Reiche
Information on the chemical composition of H. angustifolius is limited to reports of the presence of hentriacontane (Ala22), hexacosanol (Alc1), the diterpenes haplopappic acid (Dit96) and its methylester (Dit97) and the triterpenes friedelin (Tri1) and epi-friedelinol (Tri3) in the aerial parts of the plant (Silva and Sammes, 1973).
4.2 H. anthylloides Meyen & Walp
The ketone 4-hydroxyacetophenone (Ket2) is the only compound identified in the aerial parts of H. antylloides (Zdero et al., 1990).
4.3 H. arbutoides Remy
The majority of the compounds identified in the aerial parts and/or resin of H. arbutoides belong to the diterpenoids group, i.e. 15-oxo-labda-8(17),14E-diene-18-oic acid (Dit32), 15-oxo-labda-8(17),14Z-diene-18-oic acid (Dit33), labda-8(17),13E-dien-15,18-dioic acid 15-methyl ester (Dit34), 15-hydroxylabd-8(17)-en-18-oic acid (Dit35), labd-13(E)-ene-8α,15-diol (Dit62), 13R-labdane-8,15-diol (Dit63), 8α-hydroxy-ent-labd-13(14)Z-en-15-al (Dit64), 8α-hydroxylabdan-15-al (Dit65), epi-manoyl oxide (Dit68), 8,13-epoxy-14-labdeb-3-ol (Dit74), 8,13-epoxy-labdan-15-al (Dit75), 15-oxocleroda-3,13E-dien-18-oic acid (Dit91), and 15-oxocleroda-3,13Z-dien-18-oic acid (Dit92) (Zdero et al., 1991a; Rossomando et al., 1995). Additionally, the aerial parts are reported to contain 4-hydroxyacetophenone (Ket2), the sesquiterpene 1β-hydroxy-β-cyperone (Sqt44) and the flavonols santin (Flv41) and penduletin (Flv45) (Zdero et al., 1991a; Rossomando et al., 1995).
4.4 H. baylahuen Remy
The essential oil of the leaves of H. baylahuen is reported to contain eicosane (Ala11), benzene (Arh1), azulene (Arh2), naphthalene (Arh4), and the sesquiterpenes bergamotol (Sqt8) and α-cadinol (Sqt24) (Becerra et al., 2010). Phenolic compounds detected in this species include quercetin (Flv1), quercetin 3-methyl ether (Flv2), rhamnetin (Flv17), isorhamnetin (Flv18), kaempferol (Flv21), rhamnocitrin (Flv25), velutin (Flv57), sakuranetin (Flv67), persicogenin (Flv69), sternbin (Flv70), 7,4′-dimethylaromadendrin (Flv75), 7-O-methylaromadenrin (Flv76), 7,3′-di-O-methyltaxifolin (Flv81), dihydromyricetin (Flv82), prenyletin (Cum3), and 3,5-dicaffeoylquinic acid (Cin12) (Schwenker et al., 1967; Hörhammer et al., 1973; Nuñez-Alarcon et al., 1993; Vera et al., 2001; Schmeda-Hirschmann et al., 2015).
4.5 H. bezanillanus (Remy) Reiche
The compounds detected in the aerial parts of H. bezanillanus are the diterpenoid labd-13(E)-ene-8α,15-diol (Dit62), the steroid β-sitosterol (Str2) and the flavonol jaceidin 7-methyl ether (Flv44) (Maldonado et al., 1993).
4.6 H. bustillosianus Remy
The aerial parts of H. bustillosianus contain the alkenes C11H24 – C14H30 (Ala2 – Ala5), C16H34 – C33H68 (Ala7 – Ala24), along with 3-hydroxyacetophenone (Ket1) and the flavonoids santin (Flv41) and 3,6-dimethoxyapigenin (Flv48) (Urzúa et al., 2007a). Their phenolic profile includes α-linalool (Mon4), α-pinene (Mon37), β-pinene (Mon38), α-bisabolol (Sqt4), humulene (Sqt5), α-cadinene (Sqt18), γ-cadinene (Sqt20), δ-cadinene (Sqt21), (−)-isocaryophyllene (Sqt30), α-cubebene (Sqt48), β-cubebene (Sqt49), α-copaene (Sqt58), populifolic acid (Dit89) and its methyl ester (Dit90), and thunbergol (Dit102) (Urzúa et al., 2007a).
4.7 H. chrysanthemifolius (Less.) DC
The phytochemistry of H. chrysanthemifolius has been thoroughly investigated and various chemical groups of compounds have been identified in this species. Among them, in the flower heads there are present the alkanes C10H22 – C19H40 (Ala1 – Ala10), C21H44 – C33H68 (Ala12 – Ala24), 2-methyldecalin (Ala25), 2,4,6-trimethyloctane (Ala26), 2,6-dimethylundecane (Ala27), 4,6-dimethylundecane (Ala28), and 2,10-dimethylundecane Ala29) (Urzúa et al., 2007a). Furthermore, the terpenoid profile of the species includes β-myrcene (Mon3), limonene (Mon8), α-pinene (Mon37), β-pinene (Mon38), humulene (Sqt5), δ-cadinene (Sqt21), (−)-isocaryophyllene (Sqt30), β-bulgarene (Sqt31), γ-bulgarene (Sqt32), (−)-amorpha-4,11-diene (Sqt33), α-cubebene (Sqt48), β-cubebene (Sqt49), (−)-calarene (Sqt50), 1,3,4,5,6,7-hexahydro-2,5,5-trimethyl-2H-2,4a-ethanonaphthalene (Sqt51), α-copaene (Sqt58), 6α-hydroxy-ent-labd-8(17)-en-15-oic acid (Dit1), 3β-acetoxy-ent-labd-8(17)-en-15-oic acid (Dit2), and 18α-acetoxylabd-8(17)-en-15-oic acid (Dit3) (Faini et al., 1999; Urzua et al., 2007b). Regarding the phenolic compounds of H. chrysanthemifolius, it is reported the presence of quercetin (Flv1), tamarixetin (Flv3), ayanin (Flv7), myricetin 3,7,4′-trimethyl ether (Flv36), luteolin (Flv53), and diosmetin (Flv58) (Faini et al., 1999; Urzua et al., 2007b; Urzúa et al., 2012).
4.8 H. coquimbensis (Hook. & Arn.) Klingenb
The aerial parts of H. coquimbensis (syn. H. hirtellus Phil. (Klingenberg, 2007)) contain the terpenoids 7,13-labdadien-15,18-dioic acid 15-methyl ester (Dit44) and epi-friedelin (Tri2), as well as stigmasterol (Str1) (Maldonado et al., 1993). Regarding its flavonoid profile, the following compounds were detected in its aerial parts: kaempferol 7,4′-dimethyl ether (Flv27), kaempferol 3,7,4′-trimethyl ether (Flv29), pachypodol (Flv46), sakuranetin 4′-methyl ether (Flv68), eriodictyol 7,3′-dimethyl ether (Flv72), 7,4′-dimethylaromadendrin (Flv75), and 7,3′-di-O-methyltaxifolin (Flv81) (Maldonado et al., 1993).
4.9 H. deserticola Phil
In the aerial parts of H. deserticola there were detected the diterpenoids methyl-ent-4-epi-agath-18-oate (Dit17), dimethyl-ent-4-epi-agathoate (Dit18), copaiferolic acid (Dit19), copaiferolic acid 15-methyl ester (Dit20), methyl haplodesertoate (Dit26), 8α-hydroxyanticopalic acid (Dit60), 8α-hydroxyanticopalic acid methyl ester (Dit61), ent-19-hydroxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit98), and 18-acetoxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit99), along with the sesquiterpenoid germacrene D (Sqt7) (Zdero et al., 1990; Urzúa Moll et al., 1997; Tojo et al., 1999).
Regarding its phenolic composition, the aerial parts of this species are reported to contain the flavonoids quercetin (Flv1), quercetin 3-methyl ether (Flv2), isokaempferide (Flv23), 3,8-dimethylherbacetin (Flv37), 3,8,4′-trimethylherbacetin (Flv38), and 5,7,4′-trihydroxy-3,8,3′-trimethoxyflavone (Flv39); the coumarins 7-O-prenylscopoletin (Cum10), 7-O-geranylscopoletin (Cum11), O-prenylumbelliferone (Cum15) and the dimeric umbelliferone 3,3-dimethylallyl ether (Cum16), as well as the cinnamic acid derivatives chlorogenic acid (Cin10), 3,4-dicaffeoylquinic acid (Cin11), and 3,5-dicaffeoylquinic acid (Cin12) (Zdero et al., 1990; Tojo et al., 1999; Schmeda-Hirschmann et al., 2015).
4.10 H. diplopappus Remy
The resinous exudate of H. diplopappus is reported to contain the diterpenoid ent-manool (Dit9) and its 13-O-β-xylopyranoside (Dit8) (Urzua et al., 1995a).
4.11 H. foliosus (Hook. & Arn.) Hook. & Arn
H. foliosus is the species for which the greatest number of compounds has been reported. Among them, there are the alkanes C12H26 (Ala3), C14H30 (Ala5), C16H34 (Ala7), C18H38 (Ala9), and C23H48 – C33H68 (Ala14 – Ala24) (Silva and Sammes, 1973; Urzúa et al., 2000; Urzúa, 2004). Furthermore, the aerial parts of this species contain 11-tricosene (Ale1), hexacosanol (Alc1), ethylresorcinol (Alc2), diisopropyl ether (Eth1), α-asarone (Arh3), 1,2,3,4,5,6,7,8-octahydro-1-methylphenantrene (Arh5), eugenol (Arh6), styrene (Arh7), safrol (Arh8), elemicin (Arh9), dihydrobenzofuran (Arh10), benzaldehyde (Ald1), 2,3-dichloro-2-methylpropanal (Ald2), trans-2-hexenal (Ald3), nonanal (Ald4), decanal (Ald5), 3-ethylbenzaldehyde (Ald6), 4-vinylbenzaldehyde (Ald7), 3-hydroxyacetophenone (Ket1), 3-ethylacetophenone (Ket3), 4-ethylacetophenone (Ket4), dihydro-α-ionone (Ket6), 4,4-dimethyl-2-allylcyclohexanone (Ket7), (Z)-3-hexenyl acetate (Est8), tetrahydroactinidiolide (Ltn2), 4-phenyl-2-azetidinone (Ltm1), and stigmasterol (Str1) (Silva and Sammes, 1973; Urzúa et al., 2000; 2010; Urzúa, 2004; Villagra et al., 2021).
The terpenoid fraction of H. foliosus has been thoroughly studied and more than 70 compounds have been reported. Among them, there are the monoterpenoids cis-α-ocimene (Mon1), β-ocimene (Mon2), β-myrcene (Mon3), limonene (Mon8), α-terpinene (Mon9), γ-terpinene (Mon10), terpinen-4-ol (Mon11), terpinolene (Mon16), isoterpinolene (Mon17), α-terpineol (Mon18), p-menth-2-en-4-ol (Mon19), trans-p-menth-2-en-1-ol (Mon21), cis-p-menth-2-en-1-ol (Mon22), α-phellandrene (Mon25), m-cymene (Mon27), p-cymene (Mon28), p-cymen-8-ol (Mon29), o-cumenol (Mon30), 3-carene (Mon31), thujane (Mon32), α-thujene (Mon33), cis-(+/−)-4-thujanol (Mon34), 4-thujanol (Mon35), α-thujone (Mon36), α-pinene (Mon37), β-pinene (Mon38), pinocarveol (Mon39), borneol (Mon42), bornyl acetate (Mon43), camphor (Mon44), camphene (Mon45), fenchol (Mon46), 1,5-dimethyl-6-methylenespiro[2.4]heptane (Mon48), sabinene (Mon49), 5-(acetyloxy)-4,6,6-trimethyl-endobiciclo[2.2.1]heptan-2-one (Mon50), ascaridole (Mon52), and tricyclene (Mon54) (Urzúa et al., 2000; 2010; Urzúa, 2004; Villagra et al., 2021). The equally diverse sesquiterpenoid fraction includes germacrene D (Sqt7), (1α,7β,10β)-11-hydroxy-4-guaien-3-one (Sqt9), (1β,7β,10β)-1,11-dihydroxy-4-guaien-3-one (Sqt10), (1α,6α,7β,10β)-6,11-dihydroxy-4-guaien-3-one (Sqt11), α-selinene (Sqt13), γ-selinene (Sqt14), 5-eudesmen-11-ol (Sqt15), γ-eudesmol (Sqt16), cadalene (Sqt17), α-cadinene (Sqt18), β-cadinene (Sqt19), γ-cadinene (Sqt20), δ-cadinene (Sqt21), guaiol (Sqt22), 1(10),11-eremophiladiene (Sqt23), α-cadinol (Sqt24), ionene (Sqt25), 6-(1,1-dimethylethyl)-2,3-dihydro-1,1-dimethyl-3-methylene-1H-indene (Sqt26), δ-ambrinol (Sqt27), decahydro-3a,8-dimethyl-5-(1-methylethenyl)azulene (Sqt28), 1,2,3,4,5,6,7,8-octahydro-1,4-dimethyl-7-(1-methylethylidene)azulene (Sqt29), β-guaiene (Sqt36), (−)-caryophyllene (Sqt38), epi-bicyclosesquiphellandrene (Sqt39), α-muurolene (Sqt40), γ-muurolene (Sqt41), agarospirol (Sqt42), aromadendrene (Sqt47), α-cubebene (Sqt48), β-cubebene (Sqt49), spathulenol (Sqt52), β-bourbonene (Sqt55), α-copaene (Sqt58), β-copaene (Sqt59), and β-ylangene (Sqt60) (Labbé et al., 1998; Urzúa et al., 2000; 2010; Urzúa, 2004; Villagra et al., 2021). Much less diverse are the reported di- and triterpenoid profiles of the species, which include 2α-hydroxy-cis-clero-3,13(Z),8(17)-trien-15-oic acid (Dit87), 2α-acetoxy-cis-clero-3,13(Z),8(17)-trien-15-oic acid (Dit88), haplopappic acid (Dit96), friedelin (Tri1), and epi-friedelinol (Tri3) (Silva and Sammes, 1973; Urzúa et al., 2003).
The flavonoid profile of H. foliosus has also been thoroughly investigated and reported to include quercetin 3-methyl ether (Flv2), rhamnazin (Flv4), isoquercitrin (Flv10), hyperoside (Flv11), beturetol (Flv15), eupatolin (Flv16), isorhamnetin (Flv18), isorhamnetin 3-β-D-glucoside (Flv19), kaempferol (Flv21), astragalin (Flv22), isokaempferide (Flv23), kaempferol 3-methyl ether 7-β-D-glucoside (Flv24), ermanin (Flv26), kumatakenin (Flv28), haplopappin (Flv31), and haplopappin A (Flv32) (Ulubelen et al., 1982; Tschesche et al., 1985; Urzúa, 2004).
Furthermore, the following coumarins were detected in H. foliosus: esculetin (Cum1), prenyletin (Cum3), scopoletin (Cum9), and scoparone (Cum12) (Ulubelen et al., 1982; Urzúa, 2004), along with the benzoic and cinnamic acid derivatives methyl salicylate (Ben3), trans-cinnamic acid (Cin1), cis-cinnamic acid (Cin2), isobutyl-(E)-cinnamate (Cin3), pentyl-(E)-cinnamate (Cin4), benzyl-(E)-cinnamate (Cin5), and 2-phenylethyl-(E)-cinnamate (Cin6) (Urzúa et al., 2000; Urzúa, 2004; Villagra et al., 2021).
4.12 H. glutinosus Cass
The aerial parts of H. glutinosus are reported to contain 4-hydroxyacetophenone (Ket2), β-farnesene (Sqt2), germacrene D (Sqt7), 6,18-dihydroxy-ent-labd-7,13E-dien-15-oic acid (Dit41), 4-hydroxybenzoic acid (Ben1), syringic acid (Ben2), trans-cinnamic acid (Cin1), caffeic acid (Cin9), and chlorogenic acid (Cin10) (Jakupovic et al., 1986; Marambio and Silva, 1996). Furthermore, the flavonoid profile of the species includes isokaempferide (Flv23), ermanin (Flv26), santin (Flv41), jaceidin (Flv43), apigenin (Flv47), 3,6-dimethoxyapigenin (Flv48), luteolin 5- (Flv54) and 7- (Flv55) glucosides, hispidulin (Flv62), pectolinaringenin (Flv63), 3′,4′-dihydroxyflavone 5-glucoside (Flv65), and verbenacoside (Flv66) (Marambio and Silva, 1996; Valant-Vetschera and Wollenweber, 2007).
4.13 H. integerrimus (Hook. & Arn.) H.M. Hall
Scientific literature only contains information on the flavonoid profile of the leaves of H. integerrimus var. punctatus (Willd.) G.K.Br. & W.D.Clark, according to which the following compounds were detected: quercetin (Flv1), quercetin 3-methyl ether (Flv2), rhamnazin (Flv4), quercetin 3,3′-dimethyl ether (Flv5), quercetin 3,7-dimethyl ether (Flv6), isoquercitrin (Flv10), isorhamnetin (Flv18), myricetin 3′,4′dimethyl ether (Flv34), and myricetin 3,3′,4′-trimethyl ether (Flv35) (Ayanoglu et al., 1981).
4.14 H. litoralis Phil
The resin of H. litoralis is reported to contain the diterpenoids 18α-acetoxylabd-8(17)-en-15-oic acid (Dit3),18-hydroxylabd-8(17)-en-15-oic acid (Dit14), (+)-copalic acid (Dit16), and (−)-eperuic acid (Dit21) (Urzúa et al., 2004b). Moreover, the flavonols ayanin (Flv7) and retusin (Flv8) were identified in the resinous exudate of this species (Urzúa et al., 2012).
4.15 H. multifolius Reiche
The terpenoids 2,9-epoxy-p-menth-6-en-8-ol (Mon51), 9-cis-p-coumaroyloxy-α-terpineol (Sqt12), 18-hydroxylabda-7,13(E)-dien-15-oic acid (Dit39), and 18-hydroxylabda-7,13(Z)-dien-15-oic acid (Dit42) are present in the aerial parts of H. multifolius (Maatooq et al., 2002). However, the phenolic composition of this species has been more thoroughly investigated and the following compounds have been identified: quercetin (Flv1), quercetin 3-methyl ether (Flv2), isorhamnetin (Flv18), persicogenin (Flv69), sternbin (Flv70), 3-O-acetylpadmatin (Flv79), blumeatin B (Flv80), esculetin (Cum1), esculin (Cum2), prenyletin (Cum3), haplopinol (Cum4), 6-deoxyhaplopinol (Cum5), 6-hydroxy-7-(5′-hydroxy-3′,7′-dimethylocta-2′,6′-dien)-oxycoumarin (Cum6), 6-hydroxy-7-(7′-hydroxy-3′,7′-dimethylocta-2′,5′-dien)-oxycoumarin (Cum7), 6-hydroxy-7-[(E,E)-3′,7′-dimethyl-2′,4′,7′-octatrienyloxy] coumarin (Cum8), hernianin (Cum13), umbelliferone (Cum14), O-prenylumbelliferone (Cum15), and 3,5-dicaffeoylquinic acid (Cin12) (Chiang et al., 1982; Nuñez-Alarcón and Quiñones, 1995; Urzúa et al., 1995b; Maatooq et al., 2002; Torres et al., 2004; 2006; 2013; Schmeda-Hirschmann et al., 2015).
4.16 H. parvifolius (DC.) Gay
The group of compounds identified in the aerial parts of H. parvifolius includes mainly diterpenoids, as well as the sesquiterpenoids 2,8-dimethyl-2′-vinyl-5-[4-methyl-pent-3-enyl]-chromane (Sqt43) and aphanamol I (Sqt46) (Zdero et al., 1991b). The diterpenoids detected in this species are 13-hydroxylabda-6,8,14-triene (Dit27), 13-hydroxylabda-6,8(17),14-triene (Dit28), 9α,13-epoxy-labda-6,8(17),14-triene (Dit29), 6β-acetoxy-13-hydroxylabda-8,14-dien-7-one (Dit30), 6β-acetoxy-7β,13-dihydroxylabda-8,14-diene (Dit31), 6β-acetoxy-13-hydroxylabda-7,14-diene (Dit47), 13-hydroxy-6α-butyryloxylabda-7,14-diene (Dit48), 13-hydroxylabda-7,14-diene-6-one (Dit49), 9α,13-dihydroxylabda-7,14-dien-6-one (Dit50), 6α,13-dihydroxylabda-7,14-dien-17-al (Dit51), isomanool (Dit52), 6α-hydroxy-9α,13-epoxy-labda-7,14-diene (Dit53), 6α-acetoxy-9α,13-epoxy-labda-7,14-diene (Dit54), 6α-butyryloxy-9α,13-epoxy-labda-7,14-diene (Dit55), 5α-hydroxy-9α,13-epoxy-labda-7,14-diene-6-one (Dit56), 6α-acetoxy-9α,13-epoxy-labda-7,14-dien-17-al (Dit57), 6-oxo-14,15-nor-labda-7-ene (Dit58), 8α,13-dihydroxylabda-6,14-diene (Dit66), 8α,13-dihydroxylabda-5,14-dien-7-one (Dit67), epi-manoyl oxide (Dit68), 6,7-dehydro-13-epi-manoyl oxide (Dit69), 6,7-dehydro-8,13-bis-epi-manoyl oxide (Dit70), 13,17-epoxy-labda-5,7,14-triene (Dit71), 9α,13-epoxy-5α,8α-dihydroxylabda-6,14-diene (Dit72), 5α-hydroxy-7,8-epoxy-7,8-seco-6,7-dehydro-13-epi-manoyl oxide (Dit73), haploparvone (Dit103), 5α-hydroxyhaploparvone (Dit104), haploparviolide (Dit105), 1,1,5,6-tetramethyl-4-[3-hydroxy-3-methyl-pent-(4)-enyl]-tetralin (Dit106), and 1,1,5-trimethyl-6-(3-hydroxy-3-methyl-pent-4-enyl)-tetralin (Dit107) (Zdero et al., 1991b).
4.17 H. poeppigianus (Hook. & Arn.) A. Gray
The aerial parts of H. poeppigianus (syn. H. canescens (Phil.) Reiche (Klingenberg, 2007)) contain the flavonoid compounds centaureidin (Flv14), myricetin (Flv33), chrysoeriol (Flv56), diosmetin (Flv58), hispidulin (Flv62), and scutellarein 6-β-D-glucoside (Flv64) (Oksuz et al., 1981).
4.18 H. paucidentatus Phil
The aerial parts of H. paucidentatus contain 4-hydroxyacetophenone (Ket2) and the terpenoids germacrene D (Sqt7), caryophyllene oxide (Sqt34), 8-oxo-β-cyperone (Sqt45), 18-hydroxy-friedolabd-5-en-15-oic acid (Dit78), 18-hydroxy-cis-cleroda-3-en-15-oic acid (10βH, 16ξ, 19β, 17β, 20α form) (Dit83), 19-hydroxy-cis-cleroda-3-en-15-oic acid (10βH, 16ξ, 19β, 17β, 20α form) (Dit85), 18-hydroxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit93), and 18-acetoxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit99) (Jakupovic et al., 1986).
4.19 H. pulchellus DC
Regarding the compounds identified in the aerial parts of H. pulchellus, those include the diterpenoids 7α-hydroxylabd-8(17)-en-15,18-dioic acid (Dit4), labd-7-en-15,18-dioic acid (Dit36), 18-acetoxy-friedolabd-5-en-15-oic acid (Dit76), 18-acetoxy-friedolabd-5-en-7-one-15-oic acid (Dit77), 18-hydroxy-friedolabd-5-en-15-oic acid (Dit78), 18-hydroxy-7-oxo-friedolabd-5-en-15-oic acid (Dit79), friedolabd-5-en-15,18-dioic acid (Dit80), and 15-hydroxy-friedolabd-5-en-18-oic acid (Dit81) (Zdero et al., 1991a).
4.20 H. remyanus Wedd
The esters benzenepropanoic acid, 2-methyl-6-methylene-2,7-octadienyl ester (Est3), (±)-1-acetoxy-2-(p-tolyl)-2-propanol (Est4), 2-hydroxy-2-(4-methylphenyl)propyl benzenepropanoate (Est5), 2-hydroxy-2-(4-methyl-3-cyclohexen-1-yl)propyl benzenepropanoate (Est6), and 2-hydroxy-2-(4-methyl-3-cyclohexen-1-yl)propyl 3-phenyl-2-propenoate (Est7) have been detected in the aerial parts of H. remyanus (Zdero et al., 1991a). Regarding its terpenoid profile, the species contains uroterpenol (Mon12), 9-benzoyloxy-(1-formyl)-α-terpineol (Mon13), 9-benzoyloxy-α-terpineol (Mon14), 7-hydroxy-9-benzoyloxy-α-terpineol (Mon15), 8-hydroxy-9-acetoxy-β-phellandrene (Mon26), 18-hydroxylabda-7,13(E)-dien-15-oic acid (Dit39), 18-acetoxy-labda-7,13(E)-dien-15-oic acid (Dit40), and 18-dihydrocinnamoyloxy-labda-7,13E-dien-l5-oic acid (Dit46) (Zdero et al., 1991a; Faini et al., 2011). Morever, the following flavonoid compounds are present in H. remyanus: quercetin (Flv1), 3-O-acetyl-7-methylquercetin (Flv9), kaempferol 7,4′-dimethyl ether (Flv27), kaempferol 3,7,4′-trimethyl ether (Flv29), 3-O-acetyl-7,4′-dimethylkaempferol (Flv30), sakuranetin 4′-methyl ether (Flv68), eriodictyol (Flv71), pinostrobin (Flv74), 7,4′-dimethylaromadendrin (Flv75) and alpinone 3-acetate (Flv83) (Zdero et al., 1991a; Faini et al., 2011).
4.21 H. rengifoanus Remy
The aerial parts and/or leaves of H. rengifoanus are reported to contain the sesquiterpenoid liguloxide (Sqt57) and the flavonoids quercetagetin 3-methyl ether (Flv12), quercetagetin 3,7-dimethyl ether (Flv13), isorhamnetin (Flv18), isorhamnetin 3-β-D-glucoside (Flv19), isorhamnetin 3-β-D-galactoside (Flv20), apigenin (Flv47), luteolin (Flv53), and scutellarein 6-β-D-glucoside (Flv64) (Ulubelen et al., 1981; Zdero et al., 1991a).
4.22 H. rigidus Phil
The diterpenoids rigiduside (Dit6), 18-acetoxy-cis-clerode 3,13(Z)-dien-15 oic acid (Dit82), rigidusol (Dit100), and deacetylrigidusol (Dit101) are present in the aerial parts of H. rigidus (Morales et al., 2000a; 2000b; 2003). Furthermore, the flavonoids quercetin 3-methyl ether (Flv2), beturetol (Flv15), kaempferol (Flv21), isokaempferide (Flv23), sakuranetin (Flv67) and sternbin (Flv70) were detected in the aerial parts (Morales et al., 2000a; 2003; 2009; Schmeda-Hirschmann et al., 2015), along with 3,5-dicaffeoylquinic acid (Cin12) (Schmeda-Hirschmann et al., 2015).
4.23 H. schumannii (Kuntze) G.K. Br. & W.D. Clark
The alkanes C23H48 – C31H64 (Ala14 – Ala22) and C33H68 (Ala14) have been identified in the aerial parts of H. schumannii, along with 1-octadecyne (Aly1), dihydro-α-ionone (Ket6), and the lactone tetrahydroactinidiolide (Ltn2) (Urzúa et al., 2004a). The terpenoid profile of this species includes the sesquiterpenoids β-cadinene (Sqt19), β-bourbonene (Sqt55), and globulol (Sqt56), as well as the diterpenoids manool (Dit7), (−)-eperuic acid (Dit21), epi-manool (Dit25), 8α-hydroxylabdan-15-oic acid (Dit59), and 2-oxoclerod-3-en-15-oic acid (Dit86) (Urzúa et al., 1997; 2004a). Moreover, the flavonoids quercetin (Flv1), isoquercitrin (Flv10), vicenin-2 (Flv49), vitexin (Flv50), and isovitexin (Flv51) are present in the leaves of H. schumannii (Ates et al., 1982).
4.24 H. scrobiculatus (Nees) DC
The presence of the terpenoids α-farnesene (Sqt1), 18-hydroxymanool (Dit15), and 2-oxokolavenic acid (Dit94) has been reported in the case of the aerial parts and resinous exudates of H. scrobicultus (Rossomando et al., 1995; Urzúa et al., 2004b). However, the largest group of compounds in this species is that of phenolics, namely, quercetin (Flv1), isoquercitrin (Flv10), isorhamnetin (Flv18), isorhamnetin 3-β-D-glucoside (Flv19), rhamnocitrin (Flv25), santin (Flv41), eupatorin (Flv42), penduletin (Flv45), vicenin-2 (Flv49), vitexin (Flv50), isovitexin (Flv51), isoschaftoside (Flv52), eupafolin (Flv59), 6-methoxyluteolin 4′-methyl ether (Flv60), cirsiliol (Flv61), and esculetin (Cum1) (Ates et al., 1982; Rossomando et al., 1995; Urzúa et al., 2012).
4.25 H. taeda Reiche
The terpenoid profile of H. taeda includes taedol (Mon41), 18-hydroxylabda-7,13(E)-dien-15-oic acid (Dit39), 7,13-labdadien-15,18-dioic acid (Dit43), cleroda-3,13 (E)-dien-15,18-diol (Dit95), and 18-acetoxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit99) (Marambio and Silva, 1989; Torres et al., 2005; Faini et al., 2007; 2008). However, scientific literature provides more information on the phenolic composition of this species, with the following compounds being reported: quercetin (Flv1), quercetin 3-methyl ether (Flv2), quercetin 3,7-dimethyl ether (Flv6), kaempferol (Flv21), sakuranetin (Flv67), sternbin (Flv70), eriodictyol 7,3′-dimethyl ether (Flv72), eriodictyol 7,3′,4′-trimethyl ether (Flv73), 3-O-acetyl-7-O-aromadendrin (Flv77), padmatin (Flv78), 3-O-acetylpadmatin (Flv79), 9-trans-p-coumaroyloxy-α-terpineol (Cin7), 7-trans-p-coumaroyloxy-taedol (Cin8), chlorogenic acid (Cin10), 3,4-dicaffeoylquinic acid (Cin11), and 3,5-dicaffeoylquinic acid (Cin12) (Marambio and Silva, 1989; Faini et al., 2007; 2008; Schmeda-Hirschmann et al., 2015).
4.26 H. uncinatus Phil
The alkanes C23H48 – C31H64 (Ala14 – Ala22) and C33H68 (Ala14) have been identified in the resinous exudates and/or aerial parts of H. uncinatus (Urzúa et al., 2000; 2004a; 2006), along with 2,7-dimethyl-5-(1-methylethenyl)-1,8-nonadiene (Ale3) and 3,5-dihydroxy-3′,4′,6,7-tetramethoxyflavone (Flv40) (Urzúa et al., 2004a; 2006). Regarding its terpenoid profile, the species is reported to synthesize 3,3,7,7-tetramethyl-5-(2-methyl-1-propenyl)-tricyclo[4.1.0.0(2,4)]heptane (Mon53), the sesquiterpenoids cadalene (Sqt17), aromadendrene (Sqt47), α-cubebene (Sqt48), β-cubebene (Sqt49), spathulenol (Sqt52), cedryl acetate (Sqt53), β-bourbonene (Sqt55), globulol (Sqt56), α-copaene (Sqt58), as well as the clerodane diterpenoid 18-acetoxy-cis-cleroda-3-en-15-oic acid (10βH, 16ξ, 19β, 17β, 20α form) (Dit84) (Urzúa et al., 2000; 2004a; 2006).
4.27 H. velutinus Remy; H. velutinus Remy subsp. illinitus (Phil.) Klingenb
Several compounds are reported to be present in both H. velutinus and the subspecies H. velutinus subsp. illinitus. These are the alkanes C23H48 – C31H64 (Ala14 – Ala22) and C33H68 (Ala14), 5,5-dimethyl-2(5H)-furanone (Fur1), β-myrcene (Mon3), limonene (Mon8), α-pinene (Mon37), β-pinene (Mon38), labd-7-en-15,18-dioic acid-18α-methylester (Dit37), β-sitosterol (Str2), and quercetin (Flv1) (Latorre et al., 1990; Marambio and Silva, 1996; Faini et al., 2002; Urzúa et al., 2004a; Echeverría et al., 2019).
In contrast, compounds solely identified in H. velutinus include 3-ethyl-1,4-hexadiene (Ale2), 2-nonyn-1-ol (Alc3), 2-pentadecen-1-ol (Al4), n-dodecenyl-1-ol (Alc5), vanillin (Ald8), picein (Ket5), lavender lactone (Ltn1), linalyl anthranilate (Mon5), davanone (Mon6), davana ether (Mon7), 1,2:8,9-diepoxy-p-menthane (Mon19), cis-p-menth-2-en-1-ol (Mon22), trans-pulegone oxide (Mon23), α-campholenal (Mon24), m-cymene (Mon27), α-thujene (Mon33), pinocarveol (Mon39), trans-2-pinanol (Mon40), cis-verbenol (Mon47), α-sinensal (Sqt3), humulene epoxide II (Sqt6), caryophyllene oxide (Sqt34), α-guaiene (Sqt35), (−)-oplopanone (Sqt37), spathulenol (Sqt52), patchouli alcohol (Sqt54), dehydropinipholic acid 19-methyl ester (Dit11), 4α-hydroxy-18-norlabd-8(17)-en-15-oic acid (Dit12), 4β-hydroxy-19-norlabd-8(17)-en-15-oic acid (Dit13), 18-hydroxylabd-8(17)-en-15-oic acid (Dit14), 7,13-(E)-labdadien-15,18-dioic-acid-18-methyl ester (Dit45), friedelin (Tri1), epi-friedelinol (Tri3), taraxerol (Tri4), erythrodiol (Tri5), stigmasterol (Str1), isoquercitrin (Flv10), isokaempferide (Flv23), kumatakenin (Flv28), luteolin (Flv53), and scopoletin (Cum9) (Urzúa and Mendoza, 1989; Urzúa et al., 1991; Urzúa et al., 1995a; 2004a; Urzua and Mendoza, 1993; Marambio and Silva, 1996; Echeverría et al., 2019).
The group of compounds identified solely in the subspecies H. velutinus subsp. illinitus consists of 3,3,5,5-tetramethylcyclopentene (Ale4), methyl octanoate (Est1), 5-methyl-octanoic acid methyl ester (Est2), β-cadinene (Sqt19), procerin (Mer1), as well as the diterpenoids 7α-hydroxylabd-8(17)-en-15,18-dioic acid-15-methylester (Dit5), pinifolic acid 15-methyl ester (Dit22), pinifolic acid 18-methyl ester (Dit23), pinifolic acid dimethyl ester (Dit24), labd-7-en-15,18-dioic acid (Dit36), labd-7-en-15,18-dioic acid-15-methylester (Dit38), and 7-oxo-labd-8(9)-en-15,18-dioic acid-15-methylester (Dit10), (Faini et al., 2002; Urzúa et al., 2004a).
5 Traditional uses and evidence-based pharmacological activities related to human health
5.1 Traditional uses
The plants of the genus Haplopappus are of high medicinal value and form essential part of the traditional medicines of the Andean region (Chile, Argentina), where the genus presents high endemicity. Haplopappus species and their preparations have traditionally been associated with numerous health benefits, associated with multiple aspects of the human health and also with veterinary applications (Table 5).
Table 5. Traditional uses of Haplopappus species.
The main health benefits traditionally attributed to different preparations of Haplopappus plants are associated with pathologies of the human alimentary tract and metabolism. Various species and preparations have widespread use as digestives, antidiarrheic, remedies against dyspepsia, dysentery and gastrointestinal ailments, in general.
Moreover, there are reported several traditional uses associated with the human genitourinary system, with Haplopappus preparations being considered as aphrodisiacs, emmenagogues, diuretic and as remedies against urinary and renal disorders and colics or even against male and female hormonal disorders.
Other traditional uses are associated with health benefits for the human respiratory (antitussives, expectorants, cold remedies) and nervous (stimulant, antispasmodic) system, as well as with their role as disinfectants.
Finally, it is well-documented in traditional Andean medicines the use of Haplopappus preparations as cicatrizants with veterinary applications, especially to treat horses’ wounds.
It has to be mentioned that H. baylahuen Remy is recognized by the Chilean health authorities as a traditional herbal medicine against liver diseases, abdominal colics, chronic dyspepsia, kidney stones, flus and colds, as well as an aphrodisiac and wound disinfectant (Ministerio de Salud, 2010). Meanwhile, pharmaceutical products that include bailahuén, e.g., the formulations ‘Ulcenat’ and ‘Ubenat’ (Grüne Leben) and ‘Bailahuen extracto fluido’ (Knop Laboratorios S.A.) are commercialized in Chile as treatments against digestive disorders. However, there are no internationally or nationally established norms and/or protocols regarding quality, standardization, safety, and adulteration control of bailahuén preparations and commercial products.
5.2 Evidence-based pharmacological activity related to the human health
Scientific literature provides evidence related to various human health-promoting effects of extracts and isolated compounds of Haplopappus species (Table 6), with their inhibitory effect against human pathogens of bacterial origin being the most thoroughly investigated.
Table 6. Biological activity attributed to the species of the genus Haplopappus.
5.2.1 H. anthylloides meyen & walp
Although the bioactivity of the species H. anthylloides has not been extensively studied, it is reported that dichloromethane extracts of its resinous exudates present antibacterial effects, inhibiting the in vitro growth of several human pathogenic bacteria (Urzúa et al., 1995a).
5.2.2 H. baylahuen remy
Haplopappus baylahuen is the species with the highest number of bioactivity studies. Extracts and decoctions of its aerial parts are reported to have antibacterial and bactericide effects against Staphylococcus aureus, Bacillus subtilis, Acremonium falciforme (Lazo, 1990) and Salmonella enteritidis (Elgueta et al., 2021). Moreover, emulsions of its resin have been successfully used to treat the symptoms of dysentery in affected individuals (Fingland, 1903), while extracts of the aerial parts of H. baylahuen have shown anti-inflammatory (Adzet and Gene, 1991), diuretic (Méttola et al., 2018) and hepatoprotective (Nuñez-Alarcon et al., 1993) effects in rat models and hepatoprotective activity in dog models (Martin et al., 1988). The hepatoprotective effect in rats under CCl4-induced liver injury has also been confirmed in the case of 7-O-methylaromadenrin (Flv76) isolated from the aerial parts of the plant (Nuñez-Alarcon et al., 1993). Moreover, rhamnetin (Flv17) and isorhamnetin (Flv18) isolated from the leaves of H. baylahuen have been found to inhibit in a dose-dependent manner the glucose transporter GLUT1 in human cell lines and in vivo in hamsters (Vera et al., 2001). Finally, extracts of the aerial parts of this species have demonstrated significant antioxidant capacity as measured by various in vitro assays (Vogel et al., 2005a; Speisky et al., 2006; Alarcón et al., 2008; Schmeda-Hirschmann et al., 2015; Méttola et al., 2018; Elgueta et al., 2021), while also inhibiting lipid peroxidation in vitro and in erythrocyte membranes (Vogel et al., 2005a; Méttola et al., 2018).
5.2.3 H. chrysanthemifolius (Less.) DC
In the case of H. chrysanthemifolius, scientific evidence supports the antibacterial effect of the methanolic extracts of its resinous exudates, as this has been demonstrated through the in vitro growth inhibition of several Gram-positive human pathogenic bacterial strains (Urzúa et al., 2004b; 2012).
5.2.4 H. deserticola Phil
The diterpene 18-acetoxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit99) isolated from the resin of H. deserticola presented a bactericidal effect against Streptococcus mutans (Urzúa Moll et al., 1997), while the in vitro antioxidant capacity of the infusion and methanolic extract of the plant’s aerial parts has also been documented (Schmeda-Hirschmann et al., 2015).
5.2.5 H. diplopappus Remy subsp. diplopappus
The resin of H. diplopappus subsp. diplopappus, as well as the isolated diterpenoid 13-O-β-xylopyranosyl-ent-manool (Dit8) present antibacterial effect against various Gram-positive and Gram-negative human pathogenic bacteria (Urzúa et al., 1995a).
5.2.6 H. foliosus (Hook. & Arn.) Hook. & Arn
Scientific evidence supports the antibacterial effect of the resinous exudate of H. foliosus against several Gram-positive and Gram-negative human pathogenic bacteria (Urzúa et al., 1995a; 2003; Urzúa and Mendoza, 2001). Similar bioactivity has been attributed to the diterpenes 2α-hydroxy-cis-clero-3,13(Z),8(17)-trien-15-oic acid (Dit87) and 2α-acetoxy-cis-clero-3,13(Z),8(17)-trien-15-oic acid (Dit88) which were isolated from the resin of H. foliosus (Urzúa et al., 2003).
5.2.7 H. litoralis Phil
In the case of H. litoralis, it has been reported that its resinous exudate inhibits the in vitro growth of Bacillus cereus, B. subtilis, Enterococcus faecalis, Listeria monocytogens, Micrococcus luteus, S. aureus (Urzúa et al., 2004b; 2012).
5.2.8 H. multifolius reiche
Scientific literature provides evidence that support the antibacterial effect of H. multifolius resin and aerial parts extracts against a wide spectrum of Gram-positive and Gram-negative human pathogenic bacteria (Urzúa et al., 1995a; Padilla et al., 2021). Moreover, similar antibacterial activity has been documented for the coumarins esculetin (Cum1), prenyletin (Cum3) and haplopinol (Cum4) isolated from the aerial parts of this species (Chiang et al., 1982). Regarding the in vitro antioxidant capacity of H. multifolius, this has been demonstrated in the case of extracts, aerial parts infusions and resin (Vogel et al., 2005a; Schmeda-Hirschmann et al., 2015), as well as for the isolated compounds quercetin (Flv1), isorhamnetin (Flv18), prenyletin (Cum3), haplopinol (Cum4), 6-hydroxy-7-(5′-hydroxy-3′,7′-dimethylocta-2′,6′-dien)-oxycoumarin (Cum6), 6-hydroxy-7-(7′-hydroxy-3′,7′-dimethylocta-2′,5′-dien)-oxycoumarin (Cum7) and 6-hydroxy-7-[(E,E)-3′,7′-dimethyl-2′,4′,7′-octatrienyloxy] coumarin (Cum8) (Torres et al., 2006). Furthermore, the isolated compounds esculetin (Cum1), esculin (Cum2), prenyletin (Cum3), 6-hydroxy-7-(5′-hydroxy-3′,7′-dimethylocta-2′,6′-dien)-oxycoumarin (Cum6), 6-hydroxy-7-(7′-hydroxy-3′,7′-dimethylocta-2′,5′-dien)-oxycoumarin (Cum7), umbelliferone (Cum14) and O-prenylumbelliferone (Cum15) have demonstrated an anti-inflammatory effect associated to the in vitro inhibition of soybean 15-lipoxygenase (Torres et al., 2013). Finally, methanolic extracts and infusions of H. multifolius leaves inhibited the lipid peroxidation in erythrocyte membranes (Vogel et al., 2005a).
5.2.9 H. remyanus wedd
Infusions, methanolic extracts and resin from the leaves of H. remyanus demonstrated a significant antioxidant capacity in vitro, while also inhibiting lipid peroxidation in erythrocyte membranes (Vogel et al., 2005a). Furthermore, the resinous exudates of the plant exhibited an anti-inflammatory effect in mice (Faini et al., 2011) and a cytotoxic effect against T-lymphoblastic leukemia cell line (CCRF-CEM) (Faini et al., 2011).
5.2.10 H. rigidus Phil
Extracts of the aerial parts of H. rigidus have effectively inhibited the in vitro growth of several Gram-positive bacterial strains (Morales et al., 2003; Ortiz et al., 2019), presented a significant in vitro antioxidant capacity (Schmeda-Hirschmann et al., 2015) and also acted as muscle relaxants in Guinea pig models (Hnatyszyn et al., 2003). The isolated flavanone sternbin (Flv70) presented high in vitro antioxidant capacity, lipid peroxidation inhibitory effects in rat cells and also antitumoral effect against the human breast adenocarcinoma (MCF-7), human lung carcinoma (A-549) and human colon adenocarcinoma (HT–29) cell lines (Morales et al., 2009). The isolated diterpene rigidusol (Dit100) also had a cytotoxic effect on human breast adenocarcinoma cells line (MCF-7) (Morales et al., 2000b).
5.2.11 H. schumannii (Kuntze) G.K. Br. & W.D. Clark
The resinous exudates of H. schumannii inhibited the in vitro growth of several Gram-positive bacterial human pathogens (Urzúa et al., 1995a).
5.2.12 H. scrobiculatus (Nees) DC
Similarly, the only known bioactivity regarding the resin of H. scrobiculatus is that of the in vitro antibacterial effect against several Gram-positive bacteria (Urzúa et al., 1995a; 2004b; 2012).
5.2.13 H. taeda reiche
Ethanolic extracts and infusions of aerial parts of H. taeda successfully inhibited the in vitro growth of several Bacillus and Staphylococcus bacterial strains (Padilla et al., 2021). Regarding the in vitro antioxidant capacity of the species, this has been shown to be significant in the case of aerial parts infusions, extracts and resinous exudates (Vogel et al., 2005a; Schmeda-Hirschmann et al., 2015), as well as for the isolated compounds 9-trans-p-coumaroyloxy-α-terpineol (Cin7) and 7-trans-p-coumaroyloxy-taedol (Cin8) (Faini et al., 2007). Moreover, leaf infusions and methanolic extracts of H. taeda inhibited lipid peroxidation in erythrocyte membranes (Vogel et al., 2005a). Ethanolic extracts, as well as the isolated compounds taedol (Mon41), 18-acetoxy-cis-cleroda-3,13(E)-dien-15-oic acid (Dit99), and sakuranetin (Flv67) exhibited an anti-inflammatory effect against arachidonic acid-induced ear edema in mice (Faini et al., 2008).
5.2.14 H. uncinatus Phil
Extracts of the aerial parts and resinous exudates of H. uncinatus, as well as the isolated diterpenoid 18-acetoxy-cis-cleroda-3-en-15-oic acid (10βH, 16ξ, 19β, 17β, 20α form) (Dit84) have been reported to inhibit in vitro the growth of various Gram-positive human pathogenic bacteria (Urzúa et al., 1995a; 2006; Urzúa and Mendoza, 2001).
5.2.15 H. velutinus remy, H. velutinus Remy subsp. illinitus (Phil.) Klingenb
Dichloromethane extracts of the resinous exudates of H. velutinus and its subspecies H. velutinus subsp. illinitus inhibited in vitro the growth of various Gram-positive and Gram-negative human pathogenic bacteria (Urzúa et al., 1995a).
6 Non-human health related bioactivity and toxicity
Among the pharmacological activities attributed to Haplopappus species and not related to the human health, the most studied is the antimicrobial effect against plant pathogens. The essential oil of the leaves of H. baylahuen inhibited the in vitro growth of the fungi Aspergillus nigra and Fusarium oxysporum (Becerra et al., 2010). Moreover, the diterpenoid 7,13-(E)-labdadien-15,18-dioic acid 18-methyl ester (Dit45) was isolated from the resinous exudate of Haplopappus velutinus and inhibiting in vitro the mycelial growth of Botrytis cinerea (Echeverría et al., 2019). In the case of the phytopathogenic bacterium Clavibacter michiganensis subsp. michiganensis, its in vitro growth was inhibited by the resin (Urzúa and Mendoza, 2001) and the isolated diterpene 18-acetoxy-cis-cleroda-3-en-15-oic acid (10βH, 16ξ, 19β, 17β, 20α form) (Dit84) (Urzúa et al., 2006) from H. uncinatus, as well as by the methanolic extract of the resin of H. foliosus (Urzúa and Mendoza, 2001).
The essential oil of H. foliosus also exhibited insecticide effects against house flies (Musca domestica) (Urzúa et al., 2010), while hydroethanolic and chloroform extracts of H. rigidus presented biotoxic activity against Artemia salina (Morales et al., 2003).
7 Concluding remarks and future perspectives
The available scientific literature on the genus Haplopappus can be said to support, although partially, its widespread and longstanding use as a medicinal plant. However, the results of the present review highlight several limitations that need to be addressed.
Firstly, phytochemical and bioactivity research of the genus Haplopappus is largely concentrated in the 1990s and 2000s, with almost 80% of the investigation having been performed before 2010. Therefore, a revival of scientific interest and the application of modern, more advanced and diverse analytical and biological techniques can further elucidate the composition and bioactivity of Haplopappus plant species, thus broadening the existing knowledge and promoting its potential uses.
Furthermore, phytochemical and pharmacological evidence is available only for the 40% and 23%, respectively, of the botanical taxa of the genus Haplopappus, while for many of the studied taxa, the available information is rather limited. Similarly, terpenoids and phenolics correspond to approximately 70% of the compounds reported in Haplopappus spp., suggesting that scientific investigation up to date has possibly understudied other chemical groups. It is, therefore, suggested to extend the focus of scientific research to more, if not all, Haplopappus species and groups of chemical compounds, thus permitting to fully explore its promising chemical and biological prospects.
Based on the available bioactivity and pharmacological evidence, Haplopappus species can be considered as a valuable plant resource for health-promoting applications. However, the majority of the investigation provides evidence associated to the in vitro antibacterial and antioxidant activity of the genus Haplopappus. In contrast, there is a lack of scientific evidence to support or refute various traditional uses, while, at the same time, the limited number of in vivo studies and/or clinical trials hinders its wider human health-promoting application and secure use.
In this context, the information presented in the present review supports the ethnopharmacological, phytochemical and bioactive potential of the genus Haplopappus, while addressing the aforementioned limitations could further promote and broaden both scientific research and future applications and uses.
Author contributions
CM: Writing–review and editing, Writing–original draft, Methodology, Investigation, Formal Analysis, Data curation, Conceptualization. JE: Writing–review and editing, Writing–original draft, Supervision, Project administration, Methodology, Investigation, Formal Analysis, Data curation, Conceptualization.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. We gratefully acknowledge support from Comisión Nacional de Investigación Científica y Tecnológica—CONICYT PAI/ACADEMIA No. 79160109 and from Dirección de Investigación Científica y Tecnológica—DICYT USACH 022341EM_Ayudante.
Acknowledgments
CM was supported by the Scholarship Program of the Agencia Nacional de Investigación y Desarrollo de Chile (ANID Doctorado Nacional 2022/21220376).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
Publisher’s note
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Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphar.2024.1490243/full#supplementary-material
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Keywords: Haplopappus genus, ethnobotany, traditional uses, phytochemistry, pharmacology
Citation: Mitsi C and Echeverría J (2024) The genus Haplopappus: botany, phytochemistry, traditional uses, and pharmacological properties. Front. Pharmacol. 15:1490243. doi: 10.3389/fphar.2024.1490243
Received: 02 September 2024; Accepted: 07 October 2024;
Published: 21 October 2024.
Edited by:
Diego Rivera, University of Murcia, SpainReviewed by:
Katarzyna Jakimiuk, Medical University of Bialystok, PolandSerhat Sezai Cicek, Hamburg University of Applied Sciences, Germany
Copyright © 2024 Mitsi and Echeverría. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Javier Echeverría, amF2aWVyLmVjaGV2ZXJyaWFtQHVzYWNoLmNs