I. Vorobjev1,2*- 1Department of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, Kazakhstan
- 2National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan
Editorial on the Research Topic
Cytoskeletal Components as Biomarkers and Targets for Anti-Cancer Therapy: How Treatment of the Cytoskeleton can Inhibit Tumor Progression
The development of targeted chemotherapy demands detailed molecular analysis of cancers to generate precision chemotherapy programs. While standard differential cancer diagnostic is based on the morphological features of cancer tissue with the inclusion of some molecular characteristics, a more comprehensive characterization of cancer cells focused on the upregulation or downregulation of specific proteins for highly efficient therapy is needed. In response to this demand, we collected this Special Issue.
Analyzing actin cytoskeleton in the current volume, Dugina et al. discuss the differential expression of actin isoforms in cancer cells with emphasis on the correlation of expression profile with cancer cell motility. Epithelial cells express two major actin isoforms—β-actin and γ-actin with clear spatial separation between them. A growing body of evidence shows that elevated level of γ-actin directly correlates with enhanced cell motility and cancer progression. Of special interest might be the down-regulation of β-actin in tumor tissues. This raises a question about possible alternative regulation of actin isoforms during cancer development. Search for the factor(s) regulating actin isoform expression is of great interest and requires further investigation.
In cancer cells, some signaling pathways may be altered or completely lost. This feature often results in the uncontrolled growth of cancer cells. Interestingly, alteration or loss of normal regulatory pathways could be used for anti-cancer therapy. One of the examples is the loss of substrate rigidity sensing discussed by Tijore et al. in the current volume. Several proteins, including tropomyosin 2.1, filamin A, and myosin IIA involved in rigidity sensing, are downregulated during cancer development. This makes cells sensitive to mechanoptosis—a physical treatment that selectively kills cancer cells while normal counterparts easily resist it.
The ability of cancer cells to switch from mesenchymal to amoeboid types of motility allows them to obtain the enhanced capacity to form metastases after several standard anti-cancer therapy treatments like radiotherapy, the use of angiogenesis inhibitors, etc. It also happens as a side effect of well-known microtubule targeting agents (taxanes, Vinca alkaloids). Alexandrova and Lomakina, in the current volume, focus on the migratory plasticity of cancer cells and discuss the usefulness of specific proteins associated with the actin cytoskeleton, namely filamin A, fascin 1, podoplanin (PDPN), and alpha-actinins 1 and 4 as markers predicting migratory plasticity of cancer cells and possible outcome of the standard anti-cancer treatments.
Among different proteins associated with the actin cytoskeleton, Arp2/3 complex is of special interest. It is one of the master complexes driving actin polymerization at the leading edge and stimulating the formation of the cell’s lamellipodia. Since components of Arp2/3 complex are overexpressed in a variety of cancers (colorectal, liver, lung, and breast cancers) and their overexpression always correlates with poor prognosis, inhibitors of Arp2/3 components might be beneficial for targeted treatments of advanced cancers. Fokin et al. in the current volume describe the synthesis of novel potent Arp2/3 inhibitors. Remarkably, these new low molecular weight analogs of Arp 2/3 inhibitor CK-666 show a higher effect in vivo (on cultured cells and in sea urchin embryo development) while having slightly lower chemical affinity to the target molecule compared to the CK-666 itself.
Microtubules are another cytoskeletal target for anti-cancer programs. Wattanathamsan and Pongrakhananon considered the role of microtubule associated proteins (MAPs) in the microtubule dynamic and its relation to cancer prognosis. Increased dynamics of microtubules result from the up-regulation of different proteins interacting with microtubules, i.e., CAMSAP2, katanin, stathmin, and KIF14. Importantly these upregulations of the MAPs that are ubiquitously expressed in different tissues are often associated with cancer metastasis (Wang et al., 2020; Zhang et al., 2020). Authors conclude that elevated microtubule dynamics in cancer cells usually (if not always) correlate with poor prognosis. This review highlights MAPs as a novel group of prognostic markers and potential objects for targeted chemotherapy.
Combinatorial treatment using microtubule targeting agents (MTA) together with other antineoplastic agents has become a popular approach (Liang et al., 2022). However, the question of how to create the most effective combination of these drugs remains open. One opportunity is the combination of MTAs with inhibitors of anti-apoptotic proteins (Whitaker and Placzek, 2019). Suleimenov et al. in the current volume demonstrate that during mitotic arrest, cells become primed to apoptosis, and nanomolar doses of the inhibitors of anti-apoptotic protein bcl-xL, but not of other bcl-2 proteins induce death in mitosis of cells resistant against mitotic arrest. This effect is observed when the duration of mitotic arrest exceeds 4 h and cells undergoing mitotic slippage within several hours become insensitive to the bcl-xL inhibitors.
Another approach to combinatorial treatment is described in the current volume by Lafanechère et al., who discusses the use of combinations of drugs with different binding sites to the tubulin molecule. Recent data shows a synergistic effect when using novel microtubule destabilizing carbazole, Carba 1, together with microtubule stabilizer paclitaxel (PTX) (Peronne et al., 2020). This synergy allows the reduction of PTX dose, thus minimizing its side effects, and whether carbazole will be well tolerated in vivo might become a highly prospective therapy program.
Summing up, I expect that this research topic will give useful insights into our understanding of the cytoskeleton as an object for precise differential diagnostics of tumors and will be helpful for the development of novel targeted therapy programs.
Author contributions
IV wrote the entire manuscript and approved it for publication.
Acknowledgments
I appreciate the contributions of all the authors, reviewers, and editorial board members to this Research Topic.
Conflict of Interest
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
Liang, T., Lu, L., Song, X., Qi, J., and Wang, J. (2022). Combination of microtubule targeting agents with other antineoplastics for cancer treatment. Biochim. Biophys. Acta. Rev. Cancer 1877, 188777. doi:10.1016/j.bbcan.2022.188777
Peronne, L., Denarier, E., Rai, A., Prudent, R., Vernet, A., Suzanne, P., et al. (2020). Two antagonistic microtubule targeting drugs act synergistically to kill cancer cells. Cancers 12, 2196. doi:10.3390/cancers12082196
Wang, W., Zhang, R., Wang, X., Wang, N., Zhao, J., Wei, Z., et al. (2020). Suppression of KIF3A inhibits triple negative breast cancer growth and metastasis by repressing Rb-E2F signaling and epithelial-mesenchymal transition. Cancer Sci. 111, 1422–1434. doi:10.1111/cas.14324
Whitaker, R. H., and Placzek, W. J. (2019). Regulating the BCL2 family to improve sensitivity to microtubule targeting agents. Cells 8, 346. doi:10.3390/cells8040346
Zhang, L., Liu, X., Song, L., Zhai, H., and Chang, C. (2020). MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy. Cancer Cell Int. 20, 17–18. doi:10.1186/s12935-020-1095-4
Keywords: cytoskeleton, cancer markers, anti-cancer therapy, microtubules (MT), actin
Citation: Vorobjev I (2022) Editorial: Cytoskeleton in the focus of anti-cancer therapy: In a search of novel biomarkers and combinatorial therapy approaches. Front. Pharmacol. 13:1069821. doi: 10.3389/fphar.2022.1069821
Received: 14 October 2022; Accepted: 02 November 2022;
Published: 24 November 2022.
Edited and reviewed by:
Olivier Feron, Université catholique de Louvain, BelgiumCopyright © 2022 Vorobjev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: I. Vorobjev, aXZvcm9iamV2QG1haWwucnU=