This article is a correction to:
Case Report: Prenatal Diagnosis for a Rett Syndrome Family Caused by a Novel MECP2 Deletion With Heteroduplexes of PCR Product
Honghong Zhang
Yixi Sun
Yuxia Zhu1- 1Department of Pediatrics, Hangzhou Children's Hospital, Hangzhou, China
- 2Department of Reproductive Genetics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China
- 3Key Laboratory of Reproductive Genetics, Ministry of Education, Zhejiang University, Hangzhou, China
A Corrigendum on
Case Report: Prenatal Diagnosis for a Rett Syndrome Family Caused by a Novel MECP2 Deletion With Heteroduplexes of PCR Product
by Zhang, H., Sun, Y., Zhu, Y., Hong, J., and Zheng, M. (2021). Front. Pediatr. 9:748641. doi: 10.3389/fped.2021.748641
There was an omission in the text of the original article, in Case Presentation section, paragraph six. We did not provide the transcript for the deletion of MECP2, our selected transcript was MECP2 (NM_004992.4), and we have identified that the variation in our article was novel by searching in ClinVar, GnomAD, and the RettBase. A correction has been made from “In summary, we found a heterozygous deletion in c.441_1153del713 of MECP2 in the proband.” to “In summary, we found a novel heterozygous deletion in c.441_1153del713 of MECP2 (NM_004992.4) in the proband.”
In the original article, there was a mistake in Figure 1D. The base position number “1153” should be changed to “1154”. Additionally, there was also a mistake in Figure 1E. The images of Control group (PCR products untreated) were incorrect. We mistakenly replicated Heating and Reannealing group as Control group. The corrected Figure 1 appears below.
FIGURE 1
Figure 1. (A) Detection of MECP2 exon alterations via multiplex ligation-dependent probe amplification (MLPA). The arrows illustrate the presumed deletions, as the area of the peaks from the female proband DNA is approximately half that of the female controls. (B) The MECP2 amplicon with MECP2-real time quantitative PCR-2 primers to narrow down the deletion breakpoints and determine the results of MLPA. MECP2 relative amount of female proband, and male fetus were half that of female control. (C) The products of long-range PCR were analyzed with 1.5% agarose gel electrophoresis. The PCR product from the proband showed three DNA bands, whereas PCR product bands of the proband's parents and the fetus, were single. (D) Three DNA bands of long-range PCR from the proband were separately cut, T-A cloned, and finally sequenced after plasmid extraction. The sequence of top DNA band was wild type; both, bottom and middle DNA bands show 713-base pairs deletion in exon 4 (c.441_1153del713), and the single band of proband's parents and the fetus were of the wild type. (E) There was no difference between the PCR products untreated (left) and treated with heating and reannealing (right) in electrophoresis analysis. The treated PCR products were denatured at 95°C for 3 min, followed by gradual reannealing for 30 min with a temperature ramp of −1°C/min to optimize for the formation of heteroduplexes and homoduplexes. (F) Schematic diagram of three DNA bands after PCR. The top DNA band was homoduplex of wild type, the bottom DNA band was homoduplex of mutant type, the middle DNA band was heteroduplex of wild type and mutant type. Part B (red): 713-base pairs deletion in exon 4 (c.441_1153del713) of MECP2.
The authors apologize for these errors and state that these do not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: Rett syndrome, MECP2, prenatal diagnosis, mosaicism, heteroduplexes
Citation: Zhang H, Sun Y, Zhu Y, Hong J and Zheng M (2022) Corrigendum: Case Report: Prenatal Diagnosis for a Rett Syndrome Family Caused by a Novel MECP2 Deletion With Heteroduplexes of PCR Product. Front. Pediatr. 10:823860. doi: 10.3389/fped.2022.823860
Received: 28 November 2021; Accepted: 25 March 2022;
Published: 14 April 2022.
Edited and reviewed by:
Aglaia Vignoli, University of Milan, ItalyCopyright © 2022 Zhang, Sun, Zhu, Hong and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Honghong Zhang, honghong8282@126.com